A Cartilaginous Construct with Bone Collar Exerts Bone-Regenerative Property Via Rapid Endochondral Ossification

Morimoto, Shin; Kajiya, Mikihito; Yoshii, Hiroki; Yoshino, Mai; Horikoshi, Susumu; Motoike, Souta; Iwata, Takeo; Ouhara, Kazuhisa; Ando, Toshinori; Yoshimoto, Tetsuya; Shintani, Tomoaki; Mizuno, Noriyoshi

doi:10.1007/s12015-023-10554-w

Cited by 2 publications

(3 citation statements)

References 41 publications

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“…(adapted from Ref. [ 77 ]). b. BMSC pellets treated with Dioscin increase the expression of type II and type X collagen, promoting the osteochondrogenic differentiation of these cells.…”

Section: Introductionmentioning

confidence: 99%

“…For example, cartilage-like tissue with a calcified bone layer promotes healing, as demonstrated in a severe combined immunodeficiency mouse calvarial defect model ( Fig. 4 a) [ 77 ]. Furthermore, studies have shown that supplementation of BMSCs with bioactive substances such as dioscin and mangiferin (MAG) or their integration into the extracellular matrix can significantly improve bone formation via endochondral ossification in mouse models mimicking endochondral ossification ( Fig.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Exploring calcium-free alternatives in endochondral bone repair tested on In vivo trials - A review

Cárdenas-Aguazaco,

Lara-Bertrand,

Prieto-Abello

et al. 2024

Regenerative Therapy

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“…(adapted from Ref. [ 77 ]). b. BMSC pellets treated with Dioscin increase the expression of type II and type X collagen, promoting the osteochondrogenic differentiation of these cells.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exploring calcium-free alternatives in endochondral bone repair tested on In vivo trials - A review

Cárdenas-Aguazaco,

Lara-Bertrand,

Prieto-Abello

et al. 2024

Regenerative Therapy

View full text Add to dashboard Cite

“…C-MSCs, approximately 1 mm in diameter, can be directly transplanted into bone defect sites without artificial materials, inducing effective bone regeneration [18,19]. Moreover, the bone regenerative capacities of C-MSCs can be regulated by osteogenic or chondrogenic inductions [20,21]. Similarly to MSC spheroids, C-MSCs also show increased production of cell-protective factors compared to the cells on 2D plastic culture plates [22].…”

Section: Introductionmentioning

confidence: 99%

Distinctive Biological Properties between Mesenchymal Stem Cell Spheroids and Clumps of Mesenchymal Stem Cells/Extracellular Matrix Complexes in 3D Culture Systems

Yoshino,

Kajiya,

Yoshii

et al. 2023

Applied Sciences

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Background: Cells typically function and behave within a three-dimensional (3D) environment. Mesenchymal stem cells (MSCs), known for their self-renewal, multi-lineage differentiation capabilities, and paracrine effects, have garnered significant medical interest. MSC spheroid culture is widely adopted to study the biological properties of MSCs in a 3D context. In contrast, we previously developed 3D clumps of MSC/ECM complexes termed C-MSCs. C-MSCs consisted of cells and self-produced ECM proteins, allowing grafting into tissue defects without any artificial scaffolds. This present study aimed to elucidate the fundamental biological distinctions between 3D MSC spheroids and C-MSCs. Methods: MSC spheroids and C-MSCs are generated from human bone-marrow-derived MSCs. The physical properties, histological structures, and gene expression patterns were compared in vitro. Results: Macroscopic and histological examinations revealed that, whereas MSC spheroids are dense cell clusters primarily formed through Cadherin-mediated cell–cell interactions, C-MSCs are cell aggregates anchored by the ECM component COL1, enabling them to form larger structures. Furthermore, transcriptome analysis showed that C-MSCs possess enhanced capacities to produce immunomodulatory and cytoprotective factors, a prominent biological characteristic of MSCs. Conclusion: Recognizing the distinct attributes of each cell aggregate offers insights into the potential evolution of 3D cell culture techniques and possible therapeutic implications.

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A Cartilaginous Construct with Bone Collar Exerts Bone-Regenerative Property Via Rapid Endochondral Ossification

Cited by 2 publications

References 41 publications

Exploring calcium-free alternatives in endochondral bone repair tested on In vivo trials - A review

Exploring calcium-free alternatives in endochondral bone repair tested on In vivo trials - A review

Distinctive Biological Properties between Mesenchymal Stem Cell Spheroids and Clumps of Mesenchymal Stem Cells/Extracellular Matrix Complexes in 3D Culture Systems

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