A cardioactive peptide from the southern armyworm, Spodoptera eridania

Furuya, Kenji; Hackett, Murray; Cirelli, Marc A.; Schegg, Kathleen M.; Wang, Houle; Shabanowitz, Jeffrey; Hunt, Donald F.; Schooley, David A.

doi:10.1016/s0196-9781(98)00148-x

Cited by 26 publications

(15 citation statements)

References 32 publications

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“…1A). Fourteen other ENF peptides have also been identified (6,(21)(22)(23)(24)(25)(26). Comparing the primary sequences of these peptides to PSP indicated that the most variable positions were positions 4, 5, 8, 9, and 12 ( Fig.…”

Section: Sequence Comparison Of Enf Peptides and Synthesis Of Pspmentioning

confidence: 97%

Alanine-scanning Mutagenesis of Plasmatocyte Spreading Peptide Identifies Critical Residues for Biological Activity

Clark

Volkman

Thoetkiattikul

et al. 2001

Journal of Biological Chemistry

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diminished activity to a lesser degree. The point mutants Y11A, T14A, T22A, and F23A had activity identical or only slightly reduced to that of wild-type PSP. The mutant PSP-(7-23) lacked the entire unstructured domain of PSP and was found to have no plasmatocyte spreading activity. Surprisingly, E1A and N2A had higher activity than wild-type PSP, but F3A had almost no activity. We thus concluded that the lack of activity for PSP-(7-23) was largely due to the critical importance of Phe 3 . To determine whether reductions in activity correlated with alterations in tertiary structure, we compared the C7.19A, R13A, R18A, and F3A mutants to wildtype PSP by NMR spectroscopy. As expected, the simultaneous replacement of Cys 7 and Cys 19 profoundly affected tertiary structure, but the R13A, R18A, and F3A mutants did not differ from wild-type PSP. Collectively, these results indicate that residues in both the unstructured and structured domains of PSP are required for plasmatocyte-spreading activity.

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Section: Sequence Comparison Of Enf Peptides and Synthesis Of Pspmentioning

confidence: 97%

Alanine-scanning Mutagenesis of Plasmatocyte Spreading Peptide Identifies Critical Residues for Biological Activity

Clark

Volkman

Thoetkiattikul

et al. 2001

Journal of Biological Chemistry

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“…This peptide induces plasmatocytes to spread on foreign surfaces in vitro. Three years later, another homologous peptide was isolated as a cardioactive peptide (Furuya et al, 1999). We conducted parallel experiments using GBP and PSP to determine whether these peptides have distinct or multiple biological activities.…”

Section: Discovery Of Growth-blocking Pep-tide (Gbp)mentioning

confidence: 99%

“…Since GBP was found in P. separata larvae in 1990, more than 10 homologous peptides have been reported as the factors responsible for different functions, such as paralysis induction (Skinner et al, 1991(Skinner et al, , 1993, hemocyte stimulation (Clark et al, 1997;Yu et al, 1999), cell proliferation , and cardioacceleration (Furuya et al, 1999). All these peptides contain 23-25 amino acid residues, and sequence homology between GBP and other homologs is approximately 80% (Strand et al, 2000;Aizawa et al, 2001;Clark et al, 2001).…”

Section: Gbp and Gbp Homologs In Insectsmentioning

confidence: 99%

Insect cytokine growth-blocking peptide (GBP) regulates insect development

Hayakawa

2006

Appl. Entomol. Zool.

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Growth-blocking peptide (GBP) was first identified in the hemolymph of the host armyworm, Pseudaletia separata, whose growth is halted in the last instar larval stage by parasitization with the parasitoid wasp, Cotesia kariyai. Studies on the mechanism of growth retardation by GBP revealed that GBP titers in hemolymph fluctuate synchronously with dopamine levels: GBP and dopamine peaks coincide with each larval molt period, during which larvae temporarily cease moving and feeding. The fact that GBP induced the elevation of dopamine concentration in the hemolymph was demonstrated in armyworm larvae by GBP injection. Dopamine elevation was also observed in insects exposed to various stress conditions such as parasitization and chilling. These results, together with the fact that dopamine itself inhibits larval growth, indicate that GBP induces growth retardation via the elevation of dopamine levels. Further, we demonstrated that the diapause-inducing influence of short day length also elevates dopamine concentrations in hemolymphs and the brain-central nervous system (Br-CNS) of the cabbage armyworm, Mamestra brassicae. The elevation of dopamine levels contributes to the onset of pupal diapause. We therefore proposed that a GBP-dopamine system contributes to the control of growth rates in insects. Recent studies by ourselves and other laboratories have found more than 10 homologous peptides in various insect species. These peptides have diverse functions: larval growth retardation, paralysis induction, cell proliferation, immune cell stimulation and cardioacceleration. As demonstrated, GBP itself exerts most of these functions, so it is reasonable to conclude that GBP and GBP-like peptides widely present in insects should be regarded as insect cytokines with a variety of functions.

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“…ENF peptides have such biological functions as paralytic activity (Skinner et al, 1991(Skinner et al, , 1993Seino et al, 1998;Ha et al, 1999), mitogenic activity Sasagawa et al, 2001), increased ventricular rate (Furuya et al, 1999), suppression of larval growth (Hayakawa, 1990;Strand et al, 2000;Miura et al, 2002), and xenogeneic egg diapose induction (Song et al, 2001), in addition to stimulating hemocyte morphological change (Clark et al, 1997;Strand et al, 2000;Wang et al, 1999;Miura et al, 2002). Wang et al (1999) suggested that Manduca sexta paralytic peptide (MsPP) plays an important role in hemostasis.…”

Section: Archives Of Insect Biochemistry and Physiologymentioning

confidence: 99%

Effects of silkworm paralytic peptide on in vitro hematopoiesis and plasmatocyte spreading

Nakahara

Kanamori

Kiuchi

et al. 2003

Arch Insect Biochem Physiol

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Bombyx mori paralytic peptide (BmPP), a multifunctional cytokine-like molecule, is expressed in the hematopoietic organ-wing imaginal disc complex, suggesting that BmPP is involved in both immune response and the hematopoietic process. We studied the effects of BmPP on plasmatocytes and hematopoietic organs of the silkworm. BmPP (1 microM) stimulated spreading of circulating plasmatocytes, but the percentage of spread plasmatocytes was only 20%. Over 10 nM of BmPP, however, elicited prominent spreading in 70% of young plasmatocytes discharged from cultured hematopoietic organs. Cells in hematopoietic organs that were enzymatically dispersed did not spread even after adding 100 nM of BmPP, indicating that plasmatocytes acquired BmPP-sensitivity immediately after discharge. When cultured in a medium containing larval plasma, hematopoietic organs grew markedly and discharged a large number of hemocytes, over 95% of which were morphologically plasmatocytes. The hemocyte discharge was blocked in the medium containing BmPP dose-dependently, although hematopoietic organ growth was not suppressed. These results suggest that BmPP plays important roles both in hematopoietic regulation and in the hemocyte immune reaction of the silkworm.

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A cardioactive peptide from the southern armyworm, Spodoptera eridania

Cited by 26 publications

References 32 publications

Alanine-scanning Mutagenesis of Plasmatocyte Spreading Peptide Identifies Critical Residues for Biological Activity

Alanine-scanning Mutagenesis of Plasmatocyte Spreading Peptide Identifies Critical Residues for Biological Activity

Insect cytokine growth-blocking peptide (GBP) regulates insect development

Effects of silkworm paralytic peptide on in vitro hematopoiesis and plasmatocyte spreading

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