A Carboxyfluorescein-Enveloping liposome as a Physicochemical Damage Model of the Biomembrane for the Study of Lipid Peroxidation.

Zhang, Daxian; Yasuda, Tatsuji; Okada, Shigeru

doi:10.3164/jcbn.14.83

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…Localization of oxidized lipids supports this possibility. In fact, an analysis of liposomes used as a cell membrane model clearly showed that the oxidation of lipids alters the characteristics of lipid membranes in terms of fluidity and lateral diffusion ( Wong-Ekkabut et al, 2007 ), and causes the permeability of the lipid membranes to release contents from liposomes ( Zhang et al, 1993 ). In spite of this common mechanism in ferroptosis and NETosis, the morphological features of the nucleus are distinct between these two types of cell death.…”

Section: Discussionmentioning

confidence: 99%

Oxidized Phospholipids and Neutrophil Elastase Coordinately Play Critical Roles in NET Formation

Tokuhiro

Ishikawa

Sato

et al. 2021

Front. Cell Dev. Biol.

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Neutrophil extracellular traps (NETs) are web-like structures consisting of decondensed chromatin DNA and contents of granules, such as myeloperoxidase (MPO) and neutrophil elastase (NE). NETs are usually released from neutrophils undergoing NETosis, a neutrophil-specific cell death mode characterized by the collapse and disappearance of cell membranes and nuclear envelopes. It is well known that production of reactive oxygen species (ROS) triggers NETosis and NET formation. However, details of intracellular signaling downstream of ROS production during NETosis and NET formation remains uncertain. Here, we demonstrated that the peroxidation of phospholipids plays a critical role in NETosis and NET formation induced by phorbol 12-myristate13-acetate (PMA) or immune complex in vitro and by lipopolysaccharide (LPS) in vivo. This phospholipid peroxidation is mediated by the enzymatic activity of MPO. On the other hand, NE, which was previously reported to be released from granules to cytosol by MPO during NET formation, is not required for either the peroxidation of phospholipids or the execution of NETosis, but contributes to chromatin decondensation and nuclear swelling independently of MPO-mediated oxidized phospholipids. Analysis of isolated nuclei clearly demonstrated that oxidized phospholipids and NE differently yet synergistically execute chromatin decondensation and nuclear swelling, and the subsequent release of nuclear contents. These findings indicate the dual roles of MPO in NETosis and NET formation, and provide new insight into the molecular mechanism of these phenomena.

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Section: Discussionmentioning

confidence: 99%

Oxidized Phospholipids and Neutrophil Elastase Coordinately Play Critical Roles in NET Formation

Tokuhiro

Ishikawa

Sato

et al. 2021

Front. Cell Dev. Biol.

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“…The level of membrane damage and membrane rupture can be evaluated by the Carboxyfluorescein (CF) assay in liposome suspension [40] [41]. Herein, we aim to report an experimental protocol that can evaluate membranes' damage by photosensitization and protection by different types of cosmetic ingredients.…”

Section: Introductionmentioning

confidence: 99%

Performance of Cosmetic Ingredients Evaluated by Their Membrane Protection Efficiency

Silva¹,

Cabello²,

Severino³

et al. 2021

JCDSA

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Plant extracts (PEs) are frequently used as ingredients in skin and hair care products to improve protection against the excess of sun exposure. PEs are usually selected based on their antioxidant capacity for reducing the damage in lipids and proteins, which are key molecules in the maintenance of skin homeostasis and hair integrity. Interestingly, biomolecules that do not have any antioxidant capacity can have a protective effect by physically adsorbing and inhibiting leakage of membranes. Our aims are: 1) to develop an experimental protocol that quantifies the level of membrane damage/protection; 2) to recommend an approach to compare the membrane protection efficiency of different PEs; 3) to briefly describe the mechanisms of membrane protection. Evaluation of membrane damage/protection is based on a phospholipid liposome system that signals the membrane rupture caused by photo-induced lipid oxidation. Carboxyfluorescein (CF) emission is monitored as a function of time (120 min) in a controlled experimental setup, in which a certain level of membrane damage is induced by Dimethyl-Methylene Blue (DMMB) photosensitized oxidation during red-light irradiation. Percentage levels of membrane damage/protection are calculated by comparing the CF fluorescence level of DMMB-liposomes in the presence of varying concentrations of membrane-protective ingredients with that of surfactant-dissolved DMMB-liposomes. Membrane protectants reduce the level of CF fluorescence during irradiation of DMMB-liposomes. Gallic acid (GA) and trehalose (Tre) were used as standard compounds to compute the levels of protection by the antioxidant and physical adsorption mechanisms, respectively. The percentage of membrane protection was shown to be linearly proportional to the concentration of the standard protectants. The performance of PEs was measured and compared in terms of GA or Tre membrane protection equivalents. All extracted also had their antioxidant capacities evaluate by the anti-radical DPPH assay. The mem-*Contributed equally to the manuscript.

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Automated Analysis of Mitochondrial Enzymes in Cultured Skin Fibroblasts

Williams

Coakley

Christodoulou

1998

Analytical Biochemistry

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A Carboxyfluorescein-Enveloping liposome as a Physicochemical Damage Model of the Biomembrane for the Study of Lipid Peroxidation.

Cited by 5 publications

References 16 publications

Oxidized Phospholipids and Neutrophil Elastase Coordinately Play Critical Roles in NET Formation

Oxidized Phospholipids and Neutrophil Elastase Coordinately Play Critical Roles in NET Formation

Performance of Cosmetic Ingredients Evaluated by Their Membrane Protection Efficiency

Automated Analysis of Mitochondrial Enzymes in Cultured Skin Fibroblasts

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