A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol

Liu, Gang; Chen, Haode; Peng, Hongzhen; Song, Shiping; Gao, Jimin; Lu, Jinren; Ding, Min; Li, Lanying; Ren, Shuzhen; Zou, Ziying; Fan, Chunhai

doi:10.1016/j.bios.2011.07.037

Cited by 96 publications

(35 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Many studies report random binding of Abs, preventing many of the Fab fragments in the receptor platform from binding to the antigen. To solve this problem, several authors reported complex chemical systems driving the Abs connected to the solid support in a speci c orientation, [24][25][26][27][28] but in this case the many reactions involved turn this a very complex process. Also, the effectiveness of each stage of chemical modi cation conditioning such an orientation is unknown, for which this process may not be as effective as expected in the theoretical approach.…”

Section: -13mentioning

confidence: 99%

“…The use of monoclonal antibodies (mAb) may turn out to be a successful approach to obtain such a speci c response towards a selected target antigen. [28][29][30][31] This is particularly relevant when the analyte is not a single structure but a mixture of several/complex target compounds. This is the case for oxLDL, obtained by random oxidation of native LDL.…”

Section: -13mentioning

confidence: 99%

See 1 more Smart Citation

Specific label-free and real-time detection of oxidized low density lipoprotein (oxLDL) using an immunosensor with three monoclonal antibodies

et al. 2014

View full text Add to dashboard Cite

Increased levels of plasma oxLDL, which is the oxidized fraction of Low Density Lipoprotein (LDL), are associated with athero sclerosis, an inflammatory disease, and the subsequent development of severe cardiovascular diseases that are today a major cause of death in modern countries. It is therefore important to find a reliable and fast assay to determine oxLDL in serum. A new immunosensor employing three monoclonal antibodies (mAbs) against oxLDL is proposed in this work as a quick and e ffective way to monitor oxLDL.The oxLDL was first employed to produce anti-oxLDL monoclonal antibodies by hybridoma cells that were previously obtained. The immunosensor was set-up by self-assembling cysteamine (Cyst) on a gold (Au) layer (4 mm diameter) of a disposable screen-printed electrode. Three mAbs were allowed to react with N-hydroxysuccinimide (NHS) and ethyl(dimethylaminopropyl)carbodiimide (EDAC), and subsequently incubated in the Au/Cys. Albumin from bovine serum (BSA) was immobilized further to ensure that other m olecules apart from oxLDL could not bind to the electrode surface. All steps were followed by various characterization techniques such as electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The analytical operation of the immunosensor was obtained by incubating the sensing layer of the device in oxLDL for 15 minutes, prior to EIS and SWV. This was done by using standard oxLDL solutions prepared in foetal calf serum, in order to simulate patient's plasma with circulating oxLDL. A sensitive response was observed from 0.5 to 18.0 mg mL -1 . The device was successfully applied to determine the oxLDL fraction in real serum, without prior dilution or necessary chemical treatment. The use of multiple monoclonal antibodies on a biosensing platform seemed to be a s uccessful approach to produce a specific response towards a complex multi-analyte target, correlating well with the level of oxLDL within atherosclerosis disease, in a simple, fast and cheap way.

show abstract

Section: -13mentioning

confidence: 99%

Section: -13mentioning

confidence: 99%

Specific label-free and real-time detection of oxidized low density lipoprotein (oxLDL) using an immunosensor with three monoclonal antibodies

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Therefore, nanoprobes could efficiently amplify detection signals and easily achieve ultrahigh detection limit. As we know, carbon nanotubes (CNTs) possess an extremely large surface area, which could easily form biomolecular conjugation to amplify detection signals [26][27][28][29]. In this paper, we extended the application of MWNT-based colorimetric probe for ApoL1 detection.…”

Section: Introductionmentioning

confidence: 96%

Colorimetric detection and efficient monitoring of a potential biomarker of lumbar disc herniation using carbon nanotube-based probe

Huang

Liu²,

Zou

et al. 2015

Sci. China Chem.

View full text Add to dashboard Cite

Herein, a multiwalled carbon nanotubes (MWNT)-based colorimetric probe was designed to discover and monitor the level of apolipoprotein-L1 (ApoL1) in lumbar disc herniation (LDH) patients. ApoL1 could be easily found in human serum of the LDH group, but not obviously expressed in the normal control group (Ctrl), spine spondylolisthesis (SSP) group, spinal fracture (SFR) group, and spine scoliosis (SSC) group. Furthermore, the as-prepared MWNT-based probe was also used to track the recovery of LDH patients who have successful surgery operation. The P value of early diagnosis and recovery monitoring was <0.001 and >0.05 for the proposed method and a conventional enzyme-linked immunosorbent assay (ELISA), respectively, suggesting this detection strategy had significant differences compared with the traditional ELISA. All experimental results showed that ApoL1 might be a potential biomarker for early diagnosis of LDH. This proposed detection strategy has a potential application in discovering new biomarkers of diseases. colorimetric probe, apolipoprotein-L1, early diagnosis, recovery monitoring Citation: LH. Colorimetric detection and efficient monitoring of a potential biomarker of lumbar disc herniation using carbon nanotube-based probe.

show abstract

“…Several methods including liquid chromatography tandem mass spectrometry with the help of extraction process, immunosensors with desired antigen-antibody reaction and chemically modified glassy carbon electrodes with proper function of MWCNT-Nafion, graphene oxide, or Nafion-Au nanocomposite have been employed in the detection of clenbuterol [5][6][7][8][9][10][11][12][13][14]. We report here a simplified method based on a disposable screen-printed carbon electrode (SPCE) for selective clenbuterol detection without adding biorecognition element.…”

Section: Introductionmentioning

confidence: 99%

Biomolecule-free, selective detection of clenbuterol based on disposable screen-printed carbon electrode

Wong

Chen

Chang

et al. 2015

Electrochemistry Communications

View full text Add to dashboard Cite

A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol

Cited by 96 publications

References 46 publications

Specific label-free and real-time detection of oxidized low density lipoprotein (oxLDL) using an immunosensor with three monoclonal antibodies

Specific label-free and real-time detection of oxidized low density lipoprotein (oxLDL) using an immunosensor with three monoclonal antibodies

Colorimetric detection and efficient monitoring of a potential biomarker of lumbar disc herniation using carbon nanotube-based probe

Biomolecule-free, selective detection of clenbuterol based on disposable screen-printed carbon electrode

Contact Info

Product

Resources

About