A carbon dot–hemoglobin complex-based biosensor for cholesterol detection

Bui, Trang Thi; Park, Soo‐Young

doi:10.1039/c6gc00507a

Cited by 116 publications

(36 citation statements)

References 35 publications

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“…Each metallic cation would generate a distinct complex and, consequently, the three CQDs – M n+ ensembles would have a different surface. Once the literature reports the interaction between CQDs and heme‐based proteins through heme groups, each receptor may provide a differential binding event for each protein investigated. This is also true for BSA since a distinct interaction would be expected if compared to the Hb, Mb and CTC.…”

Section: Resultsmentioning

confidence: 99%

Fluorescence Based Platform to Discriminate Protein Using Carbon Quantum Dots

et al. 2019

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There is an urgent demand to develop a cheap, fast and robust methodology to sense proteins, since these biomolecules are often used as biomarker responsible for diagnosing of some diseases, such as cancer. In this regard, we report a theoretical and experimental study, as well as a cheap and effective ‘chemical‐nose’ strategy based on carbon quantum dots (CQDs) and metallic cations (M) to discriminate proteins at concentration as low as 50 nM. Thus, the CQDs were firstly synthesized through citric acid thermolysis and their characteristics were fully investigated by UV‐Vis absorption, fluorescence, infrared (FTIR), XPS and Raman spectroscopies and atomic force microscopy (AFM). These results pointed out for quasi‐spherical CQDs with diameters in the range of 1.2‐7 nm, presence of stacked graphitic layers and oxygenated functional groups, as well as disordered carbon. Based on the structural and morphological features, computational simulations were carried out to obtain a better understanding of the atomic structure. Our results evidenced a carbon‐based nanoparticle formed by stacked graphene nanoflakes containing defects due to the presence of functional groups within the graphene layers. Afterwards, a ‘tongue’‐based approach was developed by using three distinct CQDs – M (M=Fe3+, Cu2+ or Ni2+) ensembles, which allowed us to acquire different and reproducible fluorescence patterns for four proteins (bovine serum albumin, hemoglobin, myoglobin and cytochrome C) at 50 nM. Subsequently, the pattern recognition was performed using linear discriminant analysis and 36 samples were correctly identified affording 100% of accuracy.

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Section: Resultsmentioning

confidence: 99%

Fluorescence Based Platform to Discriminate Protein Using Carbon Quantum Dots

et al. 2019

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“…45 In the case of trypsin, the quenching of the fluorescence signal of the C-dots may be ascribed to π-π interactions between the aromatic ring system of the C-dots and the hydrophobic units of trypsin. 46,47 Notably, the 'free' C-dots prepared in solution exhibited an inferior degree of fluorescence quenching when exposed to trypsin (Supplementary Figure S9, Supporting Information) in comparison with the confined C-dots, underscoring the significance of the PSiO 2 matrix in promoting the adsorption of the target analytes and their subsequent effect on the embedded C-dots.…”

Section: Bimodal Detection Of Trypsin and Atpmentioning

confidence: 99%

Synthesis and characterization of a nanostructured porous silicon/carbon dot-hybrid for orthogonal molecular detection

et al. 2018

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A new hybrid guest-host material consisting of a Fabry-Pérot porous silicon (PSi) thin film, a nanostructured high surface-area matrix, and encapsulated fluorescent carbon quantum dots (C-dots) is described. The hybrid is synthesized by a facile in situ pyrolysis treatment of the carbonaceous precursor incorporated within the nanoscale pores of the inorganic host. The effects of nanoconfinement on the integrity of the C-dots and their optical properties are characterized. We show that the resulting hybrid allows for label-free optical detection of target molecules using two orthogonal modalities, that is, the white-light reflectivity of the PSi matrix and the fluorescence of the confined C-dots, and these two signals can be observed and collected simultaneously. The resulting hybrid system exhibits superior sensing performance in comparison with that of the individual components. Notably, we demonstrate that the confined C-dots exhibit greater sensitivity toward various analytes as well as an improved linear response, thus providing evidence of the impact of the host nanoscale porous scaffold on the optical properties of the C-dots. Moreover, we show that this orthogonal detection scheme increases the dynamic range of the sensor and minimizes falsenegative results.

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“…Because of their non-toxicity, environmentally friendly nature, biocompatibility and fast response times, carbon dots (CDs) has been widely used in biorelated studies, and also in designing catalytic biosensors. [10][11][12][13] Based on uorescence property, CDs have been used to sensitive detection of a diverse array of salt ions specially Fe 3+ . [14][15][16][17] It should be pointed out that in these studies, detection of metal ions is generally based on quenching CD uorescence by metal ions including Fe 3+ .…”

Section: Introductionmentioning

confidence: 99%