A carbon dot and molecular beacon based fluorometric sensor for the cancer marker microRNA-21

Mahani, Mohamad; Mousapour, Zhahra; Divsar, Faten; Nomani, Alireza; Ju, Huangxian

doi:10.1007/s00604-019-3233-z

Cited by 50 publications

(23 citation statements)

References 36 publications

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“…The detection of genes using MB is a highly sensitive and specific method, and can detect the difference of one base. This technology facilitates the detection of intracellular genetic mutations and changes in the expression of intracellular genes (Zhang et al 2019a, b;Mahani et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Detection of miR-155-5p and imaging lung cancer for early diagnosis: in vitro and in vivo study

Zhu

Fang

Guo³

et al. 2020

J Cancer Res Clin Oncol

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Purpose Currently, the routine screening program has insufficient capacity for the early diagnosis of lung cancer. Therefore, a type of chitosan-molecular beacon (CS-MB) probe was developed to recognize the miR-155-5p and image the lung cancer cells for the early diagnosis. Methods Based on the molecular beacon (MB) technology and nanotechnology, the CS-MB probe was synthesized selfassembly. There are four types of cells-three kinds of animal models and one type of histopathological sections of human lung cancer were utilized as models, including A549, SPC-A1, H446 lung cancer cells, tumor-initiating cells (TICs), subcutaneous and lung xenografts mice, and lox-stop-lox(LSL) K-ras G12D transgenic mice. The transgenic mice dynamically displayed the process from normal lung tissues to atypical hyperplasia, adenoma, carcinoma in situ, and adenocarcinoma. The different miR-155-5p expression levels in these cells and models were measured by quantitative real-time polymerase chain reaction (qRT-PCR). The CS-MB probe was used to recognize the miR-155-5p and image the lung cancer cells by confocal microscopy in vitro and by living imaging system in vivo. Results The CS-MB probe could be used to recognize the miR-155-5p and image the lung cancer cells significantly in these cells and models. The fluorescence intensity trends detected by the CS-MB probe were similar to the expression levels trends of miR-155 tested by qRT-PCR. Moreover, the fluorescence intensity showed an increasing trend with the tumor progression in the transgenic mice model, and the occurrence and development of lung cancer were dynamically monitored by the differen fluorescence intensity. In addition, the miR-155-5p in human lung cancer tissues could be detected by the miR-155-5p MB. Conclusion Both in vivo and in vitro experiments demonstrated that the CS-MB probe could be utilized to recognize the miR-155-5p and image the lung cancer cells. It provided a novel experimental and theoretical basis for the early diagnosis of the disease. Also, the histopathological sections of human lung cancer research laid the foundation for subsequent preclinical studies. In addition, different MBs could be designed to detect other miRNAs for the early diagnosis of other tumors.

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Section: Discussionmentioning

confidence: 99%

Detection of miR-155-5p and imaging lung cancer for early diagnosis: in vitro and in vivo study

Zhu

Fang

Guo³

et al. 2020

J Cancer Res Clin Oncol

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“…These results show that both of these sensors are suitable for a sensitive detection of DNA in an amplification-free format without any added complexity. These detection limits are either superior or comparable to the molecular beacon approach for nucleic acids detection without pre-amplification of targets [32,33,34,35,36].…”

Section: Resultsmentioning

confidence: 99%

“…Such a low detection limit is difficult to achieve in bulk fluorescence methods without coupling them with a target amplification or a signal amplification step. In addition, our sensors demonstrate a detection limit either superior [32,33] or comparable [34,35,36] to molecular-beacon (MB) sensors.…”

Section: Introductionmentioning

confidence: 99%

Single-Step FRET-Based Detection of Femtomoles DNA

Sapkota

Kaur

Megalathan

et al. 2019

Sensors

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Sensitive detection of nucleic acids and identification of single nucleotide polymorphism (SNP) is crucial in diagnosis of genetic diseases. Many strategies have been developed for detection and analysis of DNA, including fluorescence, electrical, optical, and mechanical methods. Recent advances in fluorescence resonance energy transfer (FRET)-based sensing have provided a new avenue for sensitive and quantitative detection of various types of biomolecules in simple, rapid, and recyclable platforms. Here, we report single-step FRET-based DNA sensors designed to work via a toehold-mediated strand displacement (TMSD) process, leading to a distinct change in the FRET efficiency upon target binding. Using single-molecule FRET (smFRET), we show that these sensors can be regenerated in situ, and they allow detection of femtomoles DNA without the need for target amplification while still using a dramatically small sample size (fewer than three orders of magnitude compared to the typical sample size of bulk fluorescence). In addition, these single-molecule sensors exhibit a dynamic range of approximately two orders of magnitude. Using one of the sensors, we demonstrate that the single-base mismatch sequence can be discriminated from a fully matched DNA target, showing a high specificity of the method. These sensors with simple and recyclable design, sensitive detection of DNA, and the ability to discriminate single-base mismatch sequences may find applications in quantitative analysis of nucleic acid biomarkers.

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“…Mahani et al developed a biosensor for the detection of ovarian cancer marker miRNA-21 based on a FRET system [ 25 ]. The sensor was constructed by a molecular beacon with a carbon QD at one end and a Black Hole Quencher 1 (BHQ1) at the other end.…”

Section: Quantum Dots-based Optical Biosensor For Circulating Cancmentioning

confidence: 99%

Recent Advancements in Nanoparticle-Based Optical Biosensors for Circulating Cancer Biomarkers

2021

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The effectiveness of cancer treatment strongly depends on the early detection of the disease. Currently, the most common diagnostic method, tissue biopsy, takes time and can be damaging to the patient. Circulating cancer biomarkers such as circulating tumor DNA, micro-RNA (miRNA), tumor proteins, exosomes, and circulating tumor cells have repeatedly demonstrated their viability as targets for minimally invasive cancer detection through liquid biopsies. However, among other things, achieving a great sensitivity of detection is still challenging due to the very low concentration of biomarkers in fluid samples. This review will discuss how the recent advances in nanoparticle-based biosensors are overcoming these practical difficulties. This report will be focusing mainly on optical transduction mechanisms of metal nanoparticles (M-NPs), quantum dots (QDs), and upconversion nanoparticles (UCNPs).

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A carbon dot and molecular beacon based fluorometric sensor for the cancer marker microRNA-21

Cited by 50 publications

References 36 publications

Detection of miR-155-5p and imaging lung cancer for early diagnosis: in vitro and in vivo study

Detection of miR-155-5p and imaging lung cancer for early diagnosis: in vitro and in vivo study

Single-Step FRET-Based Detection of Femtomoles DNA

Recent Advancements in Nanoparticle-Based Optical Biosensors for Circulating Cancer Biomarkers

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