A CAPS marker to assist selection of tomato spotted wilt virus (TSWV) resistance in pepper

Moury, Benoît; Pflieger, Stéphanie; Blattes, A.; Lefebvre, Véronique; Palloix, Alain

doi:10.1139/g99-098

Cited by 61 publications

(19 citation statements)

References 22 publications

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“…The Tsw gene has been mapped in the distal portion of chromosome 10 [136]. A CAPS marker (SCAC 568) tightly linked 0.9 cM away to the Tsw locus has been identified in a segregant F 2 population C. chinense (PI152225) × C. frutescens (PI195301) [137]. The resistance conferred by the Tsw gene is overcome by high temperatures (28-33 • C) and early plant virus inoculations (two-to four-true-leaf stages) [138].…”

Section: Thrips-transmitted Virusesmentioning

confidence: 99%

Overview of Biotic Stresses in Pepper (Capsicum spp.): Sources of Genetic Resistance, Molecular Breeding and Genomics

Parisi¹,

Alioto

Tripodi³

2020

IJMS

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Pepper (Capsicum spp.) is one of the major vegetable crops grown worldwide largely appreciated for its economic importance and nutritional value. This crop belongs to the large Solanaceae family, which, among more than 90 genera and 2500 species of flowering plants, includes commercially important vegetables such as tomato and eggplant. The genus includes over 30 species, five of which (C. annuum, C. frutescens, C. chinense, C. baccatum, and C. pubescens) are domesticated and mainly grown for consumption as food and for non-food purposes (e.g., cosmetics). The main challenges for vegetable crop improvement are linked to the sustainable development of agriculture, food security, the growing consumers’ demand for food. Furthermore, demographic trends and changes to climate require more efficient use of plant genetic resources in breeding programs. Increases in pepper consumption have been observed in the past 20 years, and for maintaining this trend, the development of new resistant and high yielding varieties is demanded. The range of pathogens afflicting peppers is very broad and includes fungi, viruses, bacteria, and insects. In this context, the large number of accessions of domesticated and wild species stored in the world seed banks represents a valuable resource for breeding in order to transfer traits related to resistance mechanisms to various biotic stresses. In the present review, we report comprehensive information on sources of resistance to a broad range of pathogens in pepper, revisiting the classical genetic studies and showing the contribution of genomics for the understanding of the molecular basis of resistance.

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Section: Thrips-transmitted Virusesmentioning

confidence: 99%

Overview of Biotic Stresses in Pepper (Capsicum spp.): Sources of Genetic Resistance, Molecular Breeding and Genomics

Parisi¹,

Alioto

Tripodi³

2020

IJMS

View full text Add to dashboard Cite

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“…In the Capsicum species, several linkage maps have been reported (Prince et al 1993, Lefebvre et al 1995, Livingstone et al 1999, Kang et al 2001. Thus, DNA markers linked to various agronomic characters were developed (Caranta et al 1999, Moury et al 2000, Blum et al 2002, Matsunaga et al 2003, Sugita et al 2004. These DNA markers are now available for MAS for some traits.…”

Section: Introductionmentioning

confidence: 99%

QTL Analysis for Resistance to Phytophthora Blight (Phytophthora capsici Leon.) Using an Intraspecific Doubled-Haploid Population of Capsicum annuum

et al. 2006

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A doubled-haploid (DH) population (n = 176) obtained by anther culture of an F 1 hybrid between a line susceptible to Phytophthora capsici 'K9-11' (Capsicum annuum L.) and a line resistant to P. capsici 'AC2258' (C. annuum L.) was inoculated with P. capsici. QTL analysis of the resistance was performed using a linkage map consisting of 16 linkage groups (LGs), covering a total distance of 1100.5 cM. Three QTLs were detected on LG1, LG6 and LG7. The QTL with the highest LOD score, detected on LG7, explained 82.7% of the phenotypic variance with a LOD score of 67.02. This QTL was designated as Phyt-1. The nearest marker was an AFLP marker, M10E3-6. The second QTL, designated as Phyt-2, was found on LG1. It explained 6.4% of the phenotypic variance with a LOD score of 2.54. The nearest RAPD marker was RP13-1. The other QTL, designated as Phyt-3, which was found on LG6, explained 5.6% of the phenotypic variance with a LOD score of 2.20. The nearest AFLP marker was M9E3-11. It was confirmed that the lines with a high resistance could be efficiently selected by using two markers, M10E3-6 and RP13-1, simultaneously. The presence of both Phyt-1 and Phyt-2 under homozygous conditions may enable to breed resistant cultivars of sweet pepper. The molecular markers identified in the present study could be useful for marker-assisted selection (MAS) in order to breed sweet pepper cultivars with a high resistance to P. capsici using 'AC2258' as a source of resistance genes.

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“…Furthermore, DNA markers linked to various useful characteristics have been identified. These markers include disease resistance to pepper mild mottle virus (PMMoV; L 4 ) (Matsunaga et al 2003), cucumber mosaic virus (Ben et al 2001a), tomato spotted wilt virus (Moury et al 2000) and potato virus Y (Caranta et al 1999), fruit shape (Ben et al 2001b), pungency (Blum et al 2002) and mature fruit color (Popovsky andParan 2000, Huh et al 2001). Moreover, we have developed SCAR markers linked to PMMoV resistance (L 3 ) (Sugita et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Rapid Construction of a Linkage Map using High-efficiency Genome Scanning/AFLP and RAPD, Based on an Intraspecific, Doubled-haploid Population of Capsicum annuum

et al. 2005

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A genetic linkage map of the sweet pepper (Capsicum annuum L.) using an intraspecific doubled-haploid (DH) population was primarily constructed by amplified fragment-length polymorphism (AFLP) using the high efficiency genome scanning (HEGS) system and random amplified polymorphic DNA (RAPD). Linkage analysis was done using a total of 518 molecular markers that consisted of 382 AFLP, 122 RAPD, 3 RFLP, 7 SCAR and 4 CAPS markers. The linkage groups consisted of 11 large linkage groups (56.7 to 118.5 cM) and 5 small linkage groups (1.8 to 33.1 cM), covering a total distance of 1043.1 cM with an average distance between 224 framework markers of 4.6 cM. AFLP markers could be developed quickly using HEGS, even in an intraspecific DH population in which it is generally difficult to detect polymorphisms in comparison with interspecific crossing populations. The map was constructed essentially in two months. Linkage analysis also provided three AFLP markers and an RAPD marker linked to PMMoV resistance (L 3 ), and an AFLP marker linked to C that was required for expression of pungency. A closer marker linked to C, Plastid-lipidAssociated Protein-simple sequence repeat (PAP-SSR), a microsatellite marker linked to C, was found at a distance of 0.6 cM. We examined the usefulness of PAP-SSR with three species in Capsicum using fragment analysis and nucleotide sequences, many alleles were found at this locus. The results suggested that these markers could be effective in marker-assisted selection (MAS) programs for sweet pepper breeding purposes.

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A CAPS marker to assist selection of tomato spotted wilt virus (TSWV) resistance in pepper

Cited by 61 publications

References 22 publications

Overview of Biotic Stresses in Pepper (Capsicum spp.): Sources of Genetic Resistance, Molecular Breeding and Genomics

Overview of Biotic Stresses in Pepper (Capsicum spp.): Sources of Genetic Resistance, Molecular Breeding and Genomics

QTL Analysis for Resistance to Phytophthora Blight (Phytophthora capsici Leon.) Using an Intraspecific Doubled-Haploid Population of Capsicum annuum

Rapid Construction of a Linkage Map using High-efficiency Genome Scanning/AFLP and RAPD, Based on an Intraspecific, Doubled-haploid Population of Capsicum annuum

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