A CAPS marker TAO1902 diagnostic for the I‐2 gene conferring resistance to Fusarium oxysporum f. sp. lycopersici race 2 in tomato

Staniaszek, Mirosława; Kozik, E.; Marczewski, Waldemar

doi:10.1111/j.1439-0523.2007.01355.x

Cited by 17 publications

(8 citation statements)

References 15 publications

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“…In Table 4 is reported the molecular characterization of both 22 selected lines and eight parents. All the results obtained were in accordance with cited literature [21][22][23][24][25][26]. The wild type S. chesmaniae resulted susceptible to all diseases except for Meloidogyne, P. syringae and TSWV.…”

Section: Molecular Characterization Of the G4 Selected Plantssupporting

confidence: 89%

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Multi-Parental Advances Generation Inter-Cross Population, to Develop Organic Tomato Genotypes by Participatory Plant Breeding

Campanelli¹,

Sestili²,

Acciarri³

et al. 2019

Agronomy

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A Multi-parent Advanced Generation Intercross (MAGIC) tomato population was developed by crossing eight founder lines chosen to include a wide range of variability. The lines were previously genotyped by a genotyping by sequencing approach. The MAGIC population was used to develop genotypes with important agronomic traits and to perform the Participatory Plant Breeding (PPB). Among the 400 plants of generation 4 (G4) of the MAGIC population cultivated in an organic field experiment, 22 individuals were phenotypically selected and a molecular analysis was done for both presence of resistance genes and fruit shape (marker assisted selection) on G5 seedlings. Three selected plants showed both the pyramiding gene of resistance to the main diseases and the ovate gene for pear shape typology. The 400 G10 stable lines that obtained from single seed descent will represent an important genetic resource for the tomato scientific community. The MAGIC population G4 was also cultivated in three organic farms located in North, Central and South Italy to carry out the PPB. The plants showed significant phenotypic differences in development, productivity and fruit color. This variability was used to select families of tomato adapted to low input crop management, different environments, agricultural practices and market conditions.

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Section: Molecular Characterization Of the G4 Selected Plantssupporting

confidence: 89%

“…A 1 Kb ladder (SIGMA) was used. The PCR cycling profiles followed those reported in the literature [21][22][23][24][25][26] for each gene detected. The molecular markers used were related to resistance genes and fruit shape characters (Table 2).…”

Section: Molecular Analysis Of the Selected G4 Plantsmentioning

confidence: 99%

Multi-Parental Advances Generation Inter-Cross Population, to Develop Organic Tomato Genotypes by Participatory Plant Breeding

Campanelli¹,

Sestili²,

Acciarri³

et al. 2019

Agronomy

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“…In recent years, rapid screening of disease-resistant lines using molecular markers in breeding studies, saving time, space and reliability by choosing the desired genotypes provides hundreds of plant choices in one day (Barone et al 2005, Staniaszek et al 2007. In the studies carried out in this context, studies of resistance to tomato against Verticillium wilt (V. dahliae Kleb.)…”

Section: Resultsmentioning

confidence: 99%

Investigation of resistance to Verticillium wilt disease (Verticillium dahliae Kleb.) in eggplant genotypes

Ateş

2020

Bitki Koruma Bülteni

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This study was conducted in 2015, it was aimed to determine the resistance status of 42 eggplant genotypes for breeding studies against Verticillium wilt disease caused by Verticillium dahliae Kleb. disease, which limits eggplant production in Turkey. Disease resistance status of eggplant genotypes were determined by classical testing. The study revealed that it was determined the severity of the disease against Verticillium dahliae varied between 9.04-76.53% among the genotypes of different eggplant species. As a result of classical testing E4, E5, E7, E8, E10, E24, E33, E42 eggplant genotypes of different species; Solanum torvum, Solanum incanum, Solanum linnaeanum, Solanum aethiopicum, Solanum sisymbriifolium, Solanum americanum have been found resistant at 7.98-9.87% disease severity. It was also determined that 22 eggplant genotypes were moderate-level resistant and 13 eggplant genotypes were in susceptible groups. Eggplant genotypes, where the resistance status of Verticillium wilt determined in the study will contribute to the development of new hybrid eggplant varieties in future.

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“…A detecção de resistência a diversas doenças do tomateiro via PCR tem sido empregada em sistemas de seleção assistida ou em estudos em diferentes patossistemas do tomateiro (El Mohtar et al, 2007;Arens et al, 2010;Dianese et al, 2010). Marcadores específicos têm sido usados com sucesso, para detecção de resistência à murcha do tomateiro causada por Fusarium (Lim et al, 2006;El Mohtar et al, 2007;Staniaszek et al, 2007;Amini, 2009;Parmar et al, 2009). No entanto, protocolos de PCR multiplex ainda não foram desenvolvidos, o que permite apenas o monitoramento de um fator de resistência por reação.…”

Section: Resultsunclassified

Detecção simultânea de fatores de resistência à murcha de fusário do tomateiro por meio de PCR multiplex

Filho

Dias

Oliveira

et al. 2016

Pesq. agropec. bras.

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Resumo: O objetivo deste trabalho foi elaborar e validar um protocolo de detecção simultânea, via reação em cadeia da polimerase multiplex (PCR multiplex), de regiões genômicas do tomateiro (Solanum lycopersicum) associadas a fatores de resistência às três raças fisiológicas de Fusarium oxysporum f. sp. lycopersici (FOL). Os pares de iniciadores empregados foram SSR-67 (específico para o gene I-1), TFusrr (específico para o gene I-2) e SSRD (específico para o gene I-3). Os resultados de genotipagem com marcadores moleculares foram comparados aos resultados de fenotipagem de uma coleção de germoplasma de tomateiro, em bioensaios de inoculação de isolados das três raças de FOL em plântulas, pelo método de imersão das raízes. A resistência ou a suscetibilidade foi confirmada por PCR, por meio de visualização dos âmplicons específicos para as regiões-alvo ligadas aos fatores de resistência às distintas raças de FOL. O protocolo elaborado para o uso conjunto dos marcadores moleculares, em PCR multiplex, permite a seleção de acessos de tomateiro resistentes às raças 1, 2, e 3 de F. oxysporum f. sp. lycopersici de maneira similar à realizada com a utilização de cada um separadamente. O PCR multiplex representa uma ferramenta viável para monitorar a incorporação desses fatores de resistência em linhagens de tomateiro.

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A CAPS marker TAO1₉₀₂ diagnostic for the I‐2 gene conferring resistance to Fusarium oxysporum f. sp. lycopersici race 2 in tomato

Cited by 17 publications

References 15 publications

Multi-Parental Advances Generation Inter-Cross Population, to Develop Organic Tomato Genotypes by Participatory Plant Breeding

Multi-Parental Advances Generation Inter-Cross Population, to Develop Organic Tomato Genotypes by Participatory Plant Breeding

Investigation of resistance to Verticillium wilt disease (Verticillium dahliae Kleb.) in eggplant genotypes

Detecção simultânea de fatores de resistência à murcha de fusário do tomateiro por meio de PCR multiplex

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