A candidate gastric stem/progenitor cell marker revealed by genome‐wide analysis

Zhu, Helen He; Zhuang, Guanglei; Gao, Wei‐Qiang

doi:10.1002/path.4601

Cited by 6 publications

(7 citation statements)

References 20 publications

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“…So we named this group as the ASPM + urothelial cells. Genome‐wide analysis has suggested ASPM as a possible gastric stem/progenitor cell marker 26,27 . In the UTI model we established, we found that ASPM + urothelial cells were significantly increased after acute injury, suggesting that ASPM + urothelial cells are involved in the bladder urothelial regeneration.…”

Section: Discussionmentioning

confidence: 74%

“…So we named this group as the ASPM + urothelial cells. Genome‐wide analysis has suggested ASPM as a possible stem/progenitor cell marker 26,27 . Immunofluorescence results showed that few ASPM + cell was found in healthy mouse urothelium (Figure 5B).…”

Section: Resultsmentioning

confidence: 99%

“…Genome-wide analysis has suggested ASPM as a possible stem/progenitor cell marker. 26,27 Immunofluorescence results showed that few ASPM + cell was found in healthy mouse urothelium (Figure 5B). However, the numbers of ASPM + basal cells were significantly increases in basal layer after UPEC injury (Figure 5C).…”

Section: Identification Of a Novel Plxna4 + Bladder Urothelial Cell Typementioning

confidence: 99%

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Single‐cell transcriptomes of mouse bladder urothelium uncover novel cell type markers and urothelial differentiation characteristics

Liu

Gao

et al. 2021

Cell Proliferation

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Objectives Much of the information to date in terms of subtypes and function of bladder urothelial cells were derived from anatomical location or by the expression of a small number of marker genes. To have a comprehensive map of the cellular anatomy of bladder urothelial cells, we performed single‐cell RNA sequencing to thoroughly characterize mouse bladder urothelium. Materials and methods A total of 18,917 single cells from mouse bladder urothelium were analysed by unbiased single‐cell RNA sequencing. The expression of the novel cell marker was confirmed by immunofluorescence using urinary tract infection models. Results Unsupervised clustering analysis identified 8 transcriptionally distinct cell subpopulations from mouse bladder urothelial cells. We discovered a novel type of bladder urothelial cells marked by Plxna4 that may be involved with host response and wound healing. We also found a group of basal‐like cells labelled by ASPM that could be the progenitor cells of adult bladder urothelium. ASPM+ urothelial cells are significantly increased after injury by UPEC. In addition, specific transcription factors were found to be associated with urothelial cell differentiation. At the last, a number of interstitial cystitis/bladder pain syndrome–regulating genes were found differentially expressed among different urothelial cell subpopulations. Conclusions Our study provides a comprehensive characterization of bladder urothelial cells, which is fundamental to understanding the biology of bladder urothelium and associated bladder disease.

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Section: Discussionmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Identification Of a Novel Plxna4 + Bladder Urothelial Cell Typementioning

confidence: 99%

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Single‐cell transcriptomes of mouse bladder urothelium uncover novel cell type markers and urothelial differentiation characteristics

Liu

Gao

et al. 2021

Cell Proliferation

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“…BUB1 co-operates with BUB1B in phosphorylation of mitotic checkpoint complex members and activating the spindle checkpoint [15], while ASPM regulates spindle organization and rotation [16]. BUB1 and ASPM functions appear to be essential for asymmetrically dividing CSC and thus are appealing as targets for selective CSC elimination [16–18]. Our analysis performed on hundreds of public cancer datasets (https://www.oncomine.org, http://biit.cs.ut.ee/mem) demonstrated that BUB1 and ASPM are overexpressed in a great variety of solid cancers within a highly conserved and substantial gene signature (>500 genes, p < E–70) (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Chromosomal abnormalities and molecular landscape of metastasizing mucinous salivary adenocarcinoma

Panaccione

Zhang

et al. 2017

Oral Oncology

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Background Mucinous adenocarcinoma of the salivary gland (MAC) is a lethal cancer with unknown molecular etiology and a high propensity to lymph node metastasis. Mostly due to its orphan status, MAC remains one of the least explored cancers that lacks cell lines and mouse models that could help translational and pre-clinical studies. Surgery with or without radiation remains the only treatment modality but poor overall survival (10-year, 44%) underscores the urgent need for mechanism-based therapies. Methods We developed the first patient-derived xenograft (PDX) model for pre-clinical MAC studies and a cell line that produces aggressively growing tumors after subcutaneous injection into nude mice. We performed cytogenetic, exome, and proteomic profiling of MAC to identify driving mutations, therapeutic targets, and pathways involved in aggressive cancers based on TCGA database mining and GEO analysis. Results: We identified in MAC KRAS (G13D) and TP53 (R213X) mutations that have been previously reported as drivers in a variety of highly aggressive cancers. Somatic mutations were also found in KDM6A, KMT2D, and other genes frequently mutated in colorectal and other cancers: FAT1, NBEA, RELN, RLP1B, and ZFHX3. Proteomic analysis of MAC implied epigenetic up-regulation of a genetic program involved in proliferation and cancer stem cell maintenance. Conclusion Genomic and proteomic analyses provided the first insight into potential molecular drivers of MAC metastases pointing at common mechanisms of CSC propagation in aggressive cancers. The in vitro/in vivo models that we created should aid in the development and validation of new treatment strategies against MAC.

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“…We read with great interest the article by Vange et al [1] in the December 2015 issue of the Journal of Pathology, as well as the subsequent commentary by Zhu et al in the January 2016 issue [2]. Vange et al identified a novel possible oxyntic stem/progenitor cell marker ASPM [Asp (abnormal spindle) homologue, microcephaly-associated (Drosophila)] by genome-wide expression analysis of the microdissected isthmus zone, where the majority of stem/progenitor cells are believed to reside.…”

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confidence: 96%

Controversial role of the possible oxyntic stem cell marker ASPM in gastric cancer

Wang

Liu

et al. 2017

The Journal of Pathology

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A candidate gastric stem/progenitor cell marker revealed by genome‐wide analysis

Cited by 6 publications

References 20 publications

Single‐cell transcriptomes of mouse bladder urothelium uncover novel cell type markers and urothelial differentiation characteristics

Single‐cell transcriptomes of mouse bladder urothelium uncover novel cell type markers and urothelial differentiation characteristics

Chromosomal abnormalities and molecular landscape of metastasizing mucinous salivary adenocarcinoma

Controversial role of the possible oxyntic stem cell marker ASPM in gastric cancer

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