A calorimetric, volumetric and combined SANS and SAXS study of hybrid siloxane phosphocholine bilayers

Frampton, Mark B.; Yakoub, Doruntina; Katsaras, John; Zelisko, Paul M.; Marquardt, Drew

doi:10.1016/j.chemphyslip.2021.105149

Cited by 2 publications

(2 citation statements)

References 37 publications

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“…However, fluidization of the membrane for DSPC:DOPC 4:6 hybrid systems is inevitable due to the predominance of DOPC and its tendency for chain disorder due to "kinks" in the tails [32,49]. To the best of the authors' opinion, the random copolymer design was unable to significantly control the bilayer disorder or orientation variability, which is what causes the fluidization effect [38,41,50].…”

Section: The Influence Of Unsaturated Dopc In Hybrid Bilayersmentioning

confidence: 99%

Development of Hybrid DSPC:DOPC:P(OEGMA950-DIPAEMA) Nanostructures: The Random Architecture of Polymeric Guest as a Key Design Parameter

et al. 2023

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Hybrid nanoparticles have gained a lot of attention due to their advantageous properties and versatility in pharmaceutical applications. In this perspective, the formation of novel systems and the exploration of their characteristics not only from a physicochemical but also from a biophysical perspective could promote the development of new nanoplatforms with well-defined features. In the current work, lipid/copolymer bilayers were formed in different lipid to copolymer ratios and examined via differential scanning calorimetry as a preformulation study to decipher the interactions between the biomaterials, followed by nanostructure preparation by the thin-film hydration method. Physicochemical and toxicological evaluations were conducted utilizing light scattering techniques, fluorescence spectroscopy, and MTS assay. 1,2-dioctadecanoyl-sn-glycero-3-phosphocholine (DSPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) in different weight ratios were the chosen lipids, while a linear random copolymer with pH- and thermoresponsive properties comprised of oligo (ethylene glycol) methyl ether methacrylate (OEGMA) and 2-(diisopropylamino) ethyl methacrylate (DIPAEMA) in different ratios was used. According to our results, non-toxic hybrid nanosystems with stimuli-responsive properties were successfully formulated, and the main parameters influencing their overall performance were the hydrophilic/hydrophobic balance, lipid to polymer ratio, and more importantly the random copolymer topology. Hopefully, this investigation can promote a better understanding of the factors affecting the behavior of hybrid systems.

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Section: The Influence Of Unsaturated Dopc In Hybrid Bilayersmentioning

confidence: 99%

Development of Hybrid DSPC:DOPC:P(OEGMA950-DIPAEMA) Nanostructures: The Random Architecture of Polymeric Guest as a Key Design Parameter

et al. 2023

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“…Cardiolipin bilayer membranes have been studied [ 139 ] as well as those formed by sphingomyelins [ 140 ], the GM3 ganglioside [ 141 ], and lipopolysaccharides extracted from cells [ 142 , 143 ]. More novel lipids have been studied using SANS, such as fully artificial ones [ 144 ] and siloxane phosphocholines [ 145 ]. SANS also provides the important membrane thickness needed for understanding the dynamics of lipid bilayers that have been measured by NSE [ 146 , 147 , 148 , 149 , 150 , 151 , 152 , 153 , 154 ], although the information reported about the structure may not be as detailed as what is found in a study dedicated to membrane structure.…”

Section: Examplesmentioning

confidence: 99%

Small-Angle Neutron Scattering for Studying Lipid Bilayer Membranes

Heller

2022

Biomolecules

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Small-angle neutron scattering (SANS) is a powerful tool for studying biological membranes and model lipid bilayer membranes. The length scales probed by SANS, being from 1 nm to over 100 nm, are well-matched to the relevant length scales of the bilayer, particularly when it is in the form of a vesicle. However, it is the ability of SANS to differentiate between isotopes of hydrogen as well as the availability of deuterium labeled lipids that truly enable SANS to reveal details of membranes that are not accessible with the use of other techniques, such as small-angle X-ray scattering. In this work, an overview of the use of SANS for studying unilamellar lipid bilayer vesicles is presented. The technique is briefly presented, and the power of selective deuteration and contrast variation methods is discussed. Approaches to modeling SANS data from unilamellar lipid bilayer vesicles are presented. Finally, recent examples are discussed. While the emphasis is on studies of unilamellar vesicles, examples of the use of SANS to study intact cells are also presented.

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A calorimetric, volumetric and combined SANS and SAXS study of hybrid siloxane phosphocholine bilayers

Cited by 2 publications

References 37 publications

Development of Hybrid DSPC:DOPC:P(OEGMA950-DIPAEMA) Nanostructures: The Random Architecture of Polymeric Guest as a Key Design Parameter

Development of Hybrid DSPC:DOPC:P(OEGMA950-DIPAEMA) Nanostructures: The Random Architecture of Polymeric Guest as a Key Design Parameter

Small-Angle Neutron Scattering for Studying Lipid Bilayer Membranes

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