A calcium requirement for electric field-induced cell shape changes and preferential orientation

Onuma, Edward K.; Hui, Sek Wen

doi:10.1016/0143-4160(85)90012-0

Cited by 45 publications

(45 citation statements)

References 29 publications

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“…However, the iplA-independent EF-induced [Ca 2+ ] i elevation is essential because removal of Ca 2+ from the extracellular medium completely prevents the electrotactic response. This may explain why most reports suggest a role for Ca 2+ signalling in electrotaxis, (Cooper and Schliwa, 1985;Onuma and Hui, 1985;Nuccitelli and Smart, 1989;Nuccitelli and Smart, 1991;Fang et al, 1998;Trollinger et al, 2002;Mycielska and Djamgoz, 2004), whereas one report reports no [Ca 2+ ] i change (Brown and Loew, 1994). This discrepancy may be accounted for by the requirement for different levels of [Ca 2+ ] i rise that are required for the electrotactic response in different cell types.…”

Section: Discussioncontrasting

confidence: 40%

“…However this suggestion has been controversial. For example, Ca 2+ dependence of electrotaxis has been observed in neural crest cells, embryo mouse fibroblasts, and fish and human keratocytes (Cooper and Schliwa, 1985;Onuma and Hui, 1985;Onuma and Hui, 1988;Nuccitelli and Smart, 1989;Nuccitelli and Smart, 1991;Fang et al, 1998;Trollinger et al, 2002). By contrast, two strains of mouse fibroblasts exhibit electrotaxis independent of Ca 2+ signalling (Brown and Loew, 1994).…”

Section: Introductionmentioning

confidence: 99%

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Influx of extracellular Ca2+ is necessary for electrotaxis inDictyostelium

Shanley

Walczysko

Bain

et al. 2006

Journal of Cell Science

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Intracellular free Ca2+ ([Ca2+](i)) is a pivotal signalling element in cell migration and is thought to be required for chemotaxis of Dictyostelium. Ca2+ signalling may also be important for electrotaxis. However this suggestion has been controversial. We show that electric fields direct Dictyostelium cells to migrate cathodally and increase [Ca2+] i in Dictyostelium cells, as determined by Fluo-3 AM imaging and Ca-45(2+) uptake. Omission of extracellular Ca2+([Ca2+](e)) and incubation with EGTA abolished the electric-field-stimulated [Ca2+](i) rise and directional cell migration. This suggests a requirement for [Ca2+](e) in the electrotactic response. Deletion of iplA, a gene responsible for chemoattractant-induced [ Ca2+](i) increase, had only a minor effect on the electric-field-induced [Ca2+](i) rise. Moreover, iplA-null Dictyostelium cells showed the same electrotactic response as wild-type cells. Therefore, iplA-independent Ca2+ influx is necessary for electrotactic cell migration. These results suggest that the [ Ca2+](i) regulatory mechanisms induced by electric fields are different from those induced by cAMP and folic acid in Dictyostelium cells. Different roles of the iplA gene in chemoattractant-induced and electrically induced Ca2+ signalling, and different effects of [ Ca2+](i) elevation on chemotaxis and electrotaxis indicate that the chemoattractant and electric cues activate distinctive initial signalling elements

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Section: Discussioncontrasting

confidence: 40%

Section: Introductionmentioning

confidence: 99%

Influx of extracellular Ca2+ is necessary for electrotaxis inDictyostelium

Shanley

Walczysko

Bain

et al. 2006

Journal of Cell Science

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“…An external EF might induce membrane depolarization and the consequent opening of plasma membrane-located, voltage-gated channels (Tester and McRobbie, 1990;Tsien et a/., 1988). Studies by Onuma and Hui (1985) and McCaig (1 989) have shown that Ca2+-channel blockers inhibit EF-induced reorientation in nerve cells and embryonic mouse fibroblasts. The origin of the Ca2' which gives rise to increases in [Ca2+], during iontophoresis is as yet unclear and requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

Role of cytosolic free calcium in the reorientation of pollen tube growth

et al. 1994

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Summary Growing pollen tubes of Agapanthus umbellatus exhibited a tip‐to‐base gradient in cytosolic free calcium ([Ca2+]c). Although this gradient is believed to be involved in pollen tube growth, its role in specifying reorientation is unknown. The direction of pollen tube growth could be modified by iontophoretic micro‐injection, electrical fields (EFs) or photolysis of caged Ca2+. Iontophoretic injection resulted in a temporary cessation of growth, an increase in [Ca2+]c and, upon recovery, reoriented growth. Weak EFs also elevated [Ca2+]c, reduced growth rates and resulted in the reorientation of pollen tubes towards the cathode. Treatment with very low concentrations of the Ca2+‐channel blocker lanthanum chloride, prior to exposure to an EF, inhibited both the increase in [Ca2+]c and reorientation whilst only slightly affecting growth rates. The responses of growth inhibition and reorientation were mimicked when [Ca2+]c was artificially elevated by photoactivating caged Ca2+ (Nitr‐5). Our data suggest that [Ca2+]c is part of a transduction mechanism which enables growing pollen tubes to successfully reorient to directional signals in the style and thus accomplish eventual fertilization of the egg.

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“…In most cases, cells move towards the cathode; for example, bovine corneal epithelial cells (Zhao et al, 1996), bovine aortic vascular endothelial cells (Li and Kolega, 2002), human retinal pigment epithelial cells (Sulik et al, 1992), human keratinocytes (Sheridan et al, 1996), amphibian neural crest cells (Cooper and Keller, 1984), C3H/10T1/2 mouse embryo fibroblasts (Onuma and Hui, 1985), fish epidermal cells (Cooper and Shliwa, 1986) and metastatic rat prostate cancer cells . However, some cell types move towards the anode; for example, human granulocytes (Rapp et al, 1988), rabbit corneal endothelial cells (Chang et al, 1996), human vascular endothelial cells (HUVECs) (Zhao et al, 2004) and metastatic human breast cancer cells (Fraser et al, 2002).…”

mentioning

confidence: 99%

Cellular mechanisms of direct-current electric field effects: galvanotaxis and metastatic disease

Mycielska

Djamgoz

2004

Journal of Cell Science

329

300

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Endogenous direct-current electric fields (dcEFs) occur in vivo in the form of epithelial transcellular potentials or neuronal field potentials, and a variety of cells respond to dcEFs in vitro by directional movement. This is termed galvanotaxis. The passive influx of Ca2+ on the anodal side should increase the local intracellular Ca2+ concentration, whereas passive efflux and/or intracellular redistribution decrease the local intracellular Ca2+ concentration on the cathodal side. These changes could give rise to `push-pull' effects, causing net movement of cells towards the cathode. However, such effects would be complicated in cells that possess voltage-gated Ca2+ channels and/or intracellular Ca2+ stores. Moreover, voltage-gated Na+ channels, protein kinases, growth factors, surface charge and electrophoresis of proteins have been found to be involved in galvanotaxis. Galvanotactic mechanisms might operate in both the short term (seconds to minutes) and the long term (minutes to hours), and recent work has shown that they might be involved in metastatic disease. The galvanotactic responses of strongly metastatic prostate and breast cancer cells are much more prominent, and the cells move in the opposite direction compared with corresponding weakly metastatic cells. This could have important implications for the metastatic process and has clinical implications. Galvanotaxis could thus play a significant role in both cellular physiology and pathophysiology.

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A calcium requirement for electric field-induced cell shape changes and preferential orientation

Cited by 45 publications

References 29 publications

Influx of extracellular Ca2+ is necessary for electrotaxis inDictyostelium

Influx of extracellular Ca2+ is necessary for electrotaxis inDictyostelium

Role of cytosolic free calcium in the reorientation of pollen tube growth

Cellular mechanisms of direct-current electric field effects: galvanotaxis and metastatic disease

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