A Calcium-Ion-Stabilized Lipase from Pseudomonas stutzeri ZS04 and its Application in Resolution of Chiral Aryl Alcohols

Abstract

An extracellular organic solvent-tolerant lipase-producing bacterium was isolated from oil-contaminated soil samples and was identified taxonomically as Pseudomonas stutzeri, from which the lipase was purified and exhibited maximal activity at temperature of 50 °C and pH of 9.0. Meanwhile, the lipase was stable below or at 30 °C and over an alkaline pH range (7.5-11.0). Ca could significantly improve the lipase thermal stability which prompts a promising application in biocatalysis through convenient medium engineering. The lipase demonstrated striking features such as distinct stability to the most tested hydrophilic and hydrophobic solvents (25 %, v/v), and DMSO could activate the lipase dramatically. In the enzyme-catalyzed resolution, lipase ZS04 manifested excellent enantioselective esterification toward the (R)-1-(4-methoxyphenyl)-ethanol (MOPE), a crucial chiral intermediate in pharmaceuticals as well as in other analogs with strict substrate specificity and theoretical highest conversion yield. This strong advantage over other related schemes made lipase ZS04 a promising biocatalyst in organic synthesis and pharmaceutical applications.

Characterization of a New Thermostable and Organic Solution-Tolerant Lipase from Pseudomonas fluorescens and Its Application in the Enrichment of Polyunsaturated Fatty Acids

“…Especially Ca 2+ and Mg 2+ increased the activity of the recombinant LipB, reaching 201.53% and 182.47%, respectively, compared to the control. The results were consistent with previous studies that found Ca 2+ and Mg 2+ to be positive factors for lipases from Pseudomonas stutzeri ZS04 [44] and Serratia sp. W3 [45].…”

“…Former studies also reported similar results. For example, lipases from P. stutzeri ZS04 [44] and B. ubonensis SL-4 [7] were also stimulated by Tween-20 and Triton X-100. However, the activity of recombinant LipB was significantly decreased after treatment with EDTA, PMSF, DTT, and CTAB, and the effect became more obvious with the increase in concentration.…”

Enzymatic Production of Biodiesel: Strategies to Overcome Methanol Inactivation

“…Under the same conditions, a lipase from Pseudomonas stutzeri retained 90% of activity. Unfortunately in this case performances in alcoholysis were not assessed [81]. Like many other lipases, the lipase from Proteus sp.…”

Identification and characterization of a novel esterase from Thauera sp.

“…Microbial organisms serve as a huge repertoire for enzyme excavation and most industrially relevant enzymes are of microbial origin. Lipolytic enzymes are important biological compounds and become increasingly popular in the food, detergent, cosmetic, pharmaceutical, and pulp industries .…”