A Calcium Antagonist Binding Site in Plants

Andrejauskas, Elisabeth; Hertel, Rainer; Marmé, Dieter

doi:10.1007/978-1-4613-2177-4_88

Cited by 2 publications

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“…Calmodulin, a Ca2+-dependent protein which was shown to activate the transport of Ca 2÷ by plasma membrane vesicles [2], enhanced Ca 2÷ uptake by plasma membranes but also by tonoplast vesicles. The Ca2+-channel blocker verapamil, which specifically binds to plant plasma membranes and, possibly, to tonoplasts [13], did not change the Ca 2÷ uptake by plasma membranes, but increased the accumulation by tonoplast preparations. In the presence of this substance, the rate of Ca 2÷ uptake by tonoplasts was higher from the beginning of the incubation.…”

Section: Ca3 + Uptake By Tonoplasts or Plasma Membranesmentioning

confidence: 80%

Calcium uptake by tonoplast and plasma membrane vesicles from spinach leaves

1988

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Tonoplast-and plasma membrane-enriched fractions were simultaneously prepared from the same crude membrane suspension from spinach leaves by free-flow elec~rophoresis. They were used for in vitro ATP-dependent Ca 2 +-uptake studies. The plasma membrane-enriched fraction accumulated Ca 2 + at a rather slow rate by a mechanism which was inhibited by orthovanadate, insensitive to nitrate and activated by calmodulin. The tonoplast-enriched fraction accumulated Ca 2 ÷ at a high rate for 5 rain, then the accumulated Ca 2 + progressively leaked out of the vesicles. The tonoplast transport system was partly inhibited by nitrate, but also by vanadate, completely abolished by the protonophore CCCP and activated by caimodulin and the Ca 2 + antagonist verapamil.

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Section: Ca3 + Uptake By Tonoplasts or Plasma Membranesmentioning

confidence: 80%

Calcium uptake by tonoplast and plasma membrane vesicles from spinach leaves

1988

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ROLE OF ASYMMETRIC CELL DIVISION IN PTERIDOPHYTE DIFFERENTIATION. II. EFFECT OF CA²⁺ ON ASYMMETRIC CELL DIVISION, RHIZOID ELONGATION, AND ANTHERIDIUM DIFFERENTIATION IN VITTARIA GEMMAE

Ruth

Kotenko

Miller

1988

American J of Botany

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Gametophytes of Vittaria graminifolia reproduce vegetatively by means of gemmae. Each gemma consists of a linear array of six cells: four body cells and a knob‐shaped terminal cell at each end. When gemmae are shed from the gametophyte onto Knop's mineral medium, the two terminal cells do not divide, but elongate to form primary rhizoids. The body cells undergo asymmetric cell division, and the smaller daughter cells differentiate into either secondary rhizoids or prothalli. When gibberellic acid is included in the medium, antheridia are formed as a result of asymmetric cell division instead of vegetative structures. We studied the effect of Ca2+ on asymmetric cell division, rhizoid elongation, and antheridium formation in gemmae cultured on Knop's mineral medium and variations of Knop's medium. Ca2+ inhibited the onset of cell division and rhizoid elongation, but was required for differentiation of antheridia. Treatments which lowered the Ca2+ content of gemmae (EGTA and dilute HCl extraction, culture on verapamil‐containing and Ca2+‐deficient medium) caused an early onset of cell division and rhizoid elongation. The stimulation of growth was most pronounced when gemmae were deprived of Ca2+ during the first 24 hr of culture. The proportion of cell divisions which differentiated into antheridia in response to GA was greatly reduced when the Ca2+ status of gemmae was lowered with verapamil and Ca2+‐EGTA buffers.

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A Calcium Antagonist Binding Site in Plants

Cited by 2 publications

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Calcium uptake by tonoplast and plasma membrane vesicles from spinach leaves

Calcium uptake by tonoplast and plasma membrane vesicles from spinach leaves

ROLE OF ASYMMETRIC CELL DIVISION IN PTERIDOPHYTE DIFFERENTIATION. II. EFFECT OF CA²⁺ ON ASYMMETRIC CELL DIVISION, RHIZOID ELONGATION, AND ANTHERIDIUM DIFFERENTIATION IN VITTARIA GEMMAE

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A Calcium Antagonist Binding Site in Plants

Cited by 2 publications

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Calcium uptake by tonoplast and plasma membrane vesicles from spinach leaves

Calcium uptake by tonoplast and plasma membrane vesicles from spinach leaves

ROLE OF ASYMMETRIC CELL DIVISION IN PTERIDOPHYTE DIFFERENTIATION. II. EFFECT OF CA2+ ON ASYMMETRIC CELL DIVISION, RHIZOID ELONGATION, AND ANTHERIDIUM DIFFERENTIATION IN VITTARIA GEMMAE

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ROLE OF ASYMMETRIC CELL DIVISION IN PTERIDOPHYTE DIFFERENTIATION. II. EFFECT OF CA²⁺ ON ASYMMETRIC CELL DIVISION, RHIZOID ELONGATION, AND ANTHERIDIUM DIFFERENTIATION IN VITTARIA GEMMAE