A Caenorhabditis elegans TGF-beta, DBL-1, controls the expression of LON-1, a PR-related protein, that regulates polyploidization and body length

Morita, Kiyokazu

doi:10.1093/emboj/21.5.1063

Cited by 95 publications

(119 citation statements)

References 58 publications

(109 reference statements)

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Section: Single-domain Proteinsmentioning

confidence: 99%

“…In C. elegans, the protein encoded by lon-1 is a target of TGF-beta signalling and is expressed in hypodermal tissues; the lon-1 gene is epistatic to dbl-1 and negatively regulated by Sma/Mab pathway signalling (Maduzia et al, 2002;Morita et al, 2002;Tuck et al, 2014). The disruption of lon-1 function in C. elegans by dsRNAi results in long worms, indicating that it is a negative regulator of body length (Morita et al, 2002). Ce-lon-1 encodes a 312 amino acid protein with a sequence motif (GHYVQVVW) that is conserved across many metazoan organisms including H. contortus (e.g., Kasahara et al, 1989;Lu et al, 1993;Murphy et al, 1995;Kjeldsen et al, 1996;Morita et al, 2002;Delannoy-Normand et al, 2010).…”

Section: Single-domain Proteinsmentioning

confidence: 99%

“…The disruption of lon-1 function in C. elegans by dsRNAi results in long worms, indicating that it is a negative regulator of body length (Morita et al, 2002). Ce-lon-1 encodes a 312 amino acid protein with a sequence motif (GHYVQVVW) that is conserved across many metazoan organisms including H. contortus (e.g., Kasahara et al, 1989;Lu et al, 1993;Murphy et al, 1995;Kjeldsen et al, 1996;Morita et al, 2002;Delannoy-Normand et al, 2010). Expression studies have also shown that Ce-LON-1 is repressed by Ce-DBL-1, suggesting that Ce-LON-1 is a downstream component of the TGF-beta growth regulation pathway (Morita et al, 2002).…”

Section: Single-domain Proteinsmentioning

confidence: 99%

“…Ce-lon-1 encodes a 312 amino acid protein with a sequence motif (GHYVQVVW) that is conserved across many metazoan organisms including H. contortus (e.g., Kasahara et al, 1989;Lu et al, 1993;Murphy et al, 1995;Kjeldsen et al, 1996;Morita et al, 2002;Delannoy-Normand et al, 2010). Expression studies have also shown that Ce-LON-1 is repressed by Ce-DBL-1, suggesting that Ce-LON-1 is a downstream component of the TGF-beta growth regulation pathway (Morita et al, 2002). LON-1 is expressed mainly in adult and larval stages (as is the case in H. contortus) and has dosedependent effects on body length linked to hypodermal ploidy (cf.…”

Section: Single-domain Proteinsmentioning

confidence: 99%

“…LON-1 is expressed mainly in adult and larval stages (as is the case in H. contortus) and has dosedependent effects on body length linked to hypodermal ploidy (cf. Morita et al, 2002).…”

Section: Single-domain Proteinsmentioning

confidence: 99%

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The barber's pole worm CAP protein superfamily — A basis for fundamental discovery and biotechnology advances

Mohandas

Young

Jabbar

et al. 2015

Biotechnology Advances

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Parasitic worm proteins that belong to the cysteine-rich secretory proteins, antigen 5 and pathogenesis-related 1 (CAP) superfamily are proposed to play key roles in the infection process and the modulation of immune responses in host animals. However, there is limited information on these proteins for most socio-economically important worms. Here, we review the CAP protein superfamily of Haemonchus contortus (barber's pole worm), a highly significant parasitic roundworm (order Strongylida) of small ruminants. To do this, we mined genome and transcriptomic datasets, predicted and curated full-length amino acid sequences (n = 45), undertook systematic phylogenetic analyses of these data and investigated transcription throughout the life cycle of H. contortus. We inferred functions for selected C. elegans orthologs (including vap-1, vap-2, scl-5 and lon-1) based on genetic networking and by integrating data and published information, and were able to infer that a subset of orthologs and their interaction partners play pivotal roles in growth and development via the insulin-like and/or the TGF-beta signaling pathways. The identification of the important and conserved growth regulator LON-1 led us to appraise the threedimensional structure of this CAP protein by comparative modelling. This model revealed the presence of different topological moieties on the canonical fold of the CAP domain, which coincide with an overall charge separation as indicated by the electrostatic surface potential map. These observations suggest the existence of separate sites for effector binding and receptor interactions, and thus support the proposal that these worm molecules act in similar ways as venoms act as ligands for chemokine receptors or G protein-coupled receptor effectors. In conclusion, this review should guide future molecular studies of these molecules, and could support the development of novel interventions against haemonchosis.

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Section: Single-domain Proteinsmentioning

confidence: 99%

Section: Single-domain Proteinsmentioning

confidence: 99%

Section: Single-domain Proteinsmentioning

confidence: 99%

Section: Single-domain Proteinsmentioning

confidence: 99%

“…LON-1 is expressed mainly in adult and larval stages (as is the case in H. contortus) and has dosedependent effects on body length linked to hypodermal ploidy (cf. Morita et al, 2002).…”

Section: Single-domain Proteinsmentioning

confidence: 99%

See 3 more Smart Citations

The barber's pole worm CAP protein superfamily — A basis for fundamental discovery and biotechnology advances

Mohandas

Young

Jabbar

et al. 2015

Biotechnology Advances

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A small issue addressed

Gumienny

Padgett

2003

BioEssays

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Cell size is an important determinant of body size. While the genetic mechanisms of cell size regulation have been well studied in yeast, this process has only recently been addressed in multicellular organisms. One recent report by Wang et al. (2002) shows that in the nematode C. elegans, the TGFbeta-like pathway acts in the hypodermis to regulate cell size and consequently body size.1 This finding is an exciting step in discovering the molecular mechanisms that control cell and body size.

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Dramatic evolution of body length due to postembryonic changes in cell size in a newly discovered close relative ofCaenorhabditis elegans

2018

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Understanding morphological diversity—and morphological constraint—has been a central question in evolutionary biology since its inception. Nematodes of the genus Caenorhabditis, which contains the well‐studied model organism C. elegans, display remarkable morphological consistency in the face of extensive genetic divergence. Here, we provide a description of the broad developmental patterns of a newly discovered species, C. sp. 34, which was isolated from fresh figs in Okinawa and which is among the closest known relatives of C. elegans. C. sp. 34 displays an extremely large body size; it can grow to be nearly twice as long as C. elegans and all other known members of the genus. Observations of the timing of developmental milestones reveal that C. sp. 34 develops about twice as slowly as C. elegans. Measurements of embryonic and larval size show that the size difference between C. sp. 34 and C. elegans is largely due to postembryonic events, particularly during the transition from larval to adult stages. This difference in size is not attributable to differences in germ line chromosome number or the number of somatic cells. The overall difference in body size is therefore largely attributable to changes in cell size via increased cytoplasmic volume. Because of its close relationship to C. elegans, the distinctness of C. sp. 34 provides an ideal system for the detailed analysis of evolutionary diversification. The context of over 40 years of C. elegans developmental genetics also reveals clues into how natural selection and developmental constraint act jointly to promote patterns of morphological stasis and divergence in this group.

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A Caenorhabditis elegans TGF-beta, DBL-1, controls the expression of LON-1, a PR-related protein, that regulates polyploidization and body length

Cited by 95 publications

References 58 publications

The barber's pole worm CAP protein superfamily — A basis for fundamental discovery and biotechnology advances

The barber's pole worm CAP protein superfamily — A basis for fundamental discovery and biotechnology advances

A small issue addressed

Dramatic evolution of body length due to postembryonic changes in cell size in a newly discovered close relative ofCaenorhabditis elegans

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