A C‐type CpG ODN accelerates wound healing via regulating fibroblasts and immune response

Li, Lina; Xu, Zhenyu; Zuo, Jian; Ding, Jin

doi:10.1002/jcb.28061

Cited by 9 publications

(7 citation statements)

References 24 publications

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“…CpG-ODN received group presented uniformly healing pattern over 21 days without sepsis and formation of unhealthy granulation tissue formation. This data get in accordance with [33] who found that CpG ODN increased collagen I production, encouraged cell proliferation in human skin fibroblasts cells and activate immune cells .…”

Section: Discussionsupporting

confidence: 92%

Modulation of immune response in canine chronic wound stimulated with Class C CpG oligonucleotide

Khalifa

M.Alakraa

Elkasapy

2022

Benha Journal of Applied Sciences

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Chronic wound healing is a severe problem in veterinary practice, therefore, a new agent for wound healing is recommended. Synthetic oligodeoxynucleotides containing one or more CpG motifs (CpG-ODN) are used to stimulate the immune system and improve skin wound healing. Ten clinically healthy animals were used to estimate and compare the rate of wound healing. The animals were arranged into two groups (five animals each). The estimation of wound healing was carried out by clinical observation of the signs of healing in 21 days, the observation was based on the presence of sepsis and unhealthy granulation tissue formation. Histopathological findings depend on the rate of new vascularization, amount of collagen bundles, epithelial thickness and cellular component. Molecular assessment based on the expression of IL10 & TGFβ. This study aimed to evaluate the role of Class C CpG oligonucleotide as new modality to overcome the delay of chronic wound healing in canine.

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Section: Discussionsupporting

confidence: 92%

Modulation of immune response in canine chronic wound stimulated with Class C CpG oligonucleotide

Khalifa

M.Alakraa

Elkasapy

2022

Benha Journal of Applied Sciences

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“…In addition, the signaling triggered via TLR9 contributes to the accelerated skin wound-healing. Specifically, mice triggered by CpG ODN, a TLR9 ligand, exhibited accelerated skin wound-healing together with increased production of TNF-α, IL-6, VEGF, and TGF-β in the wounded tissues [ 45 , 46 ], similar to the heat-killed KH2-treated mice in the current study. Collectively, these findings suggest that TLR9-expressing cells may recognize heat-killed KH2 for the induction of inflammatory cytokine and growth factor production and the promotion of skin wound-healing responses.…”

Section: Discussionmentioning

confidence: 74%

Topical Administration of Heat-Killed Enterococcus faecalis Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing

et al. 2021

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Lactic acid bacteria (LAB) are known to have beneficial effects on immune responses when they are orally administered as bacterial products. Although the beneficial effects of LAB have been reported for the genera Lactobacillus and Lactococcus, little has been uncovered on the effects of the genus Enterococcus on skin wound-healing. In this study, we aimed to clarify the effect of heat-killed Enterococcus faecalis KH2 (heat-killed KH2) strain on the wound-healing process and to evaluate the therapeutic potential in chronic skin wounds. We analyzed percent wound closure, re-epithelialization, and granulation area, and cytokine and growth factor production. We found that heat-killed KH2 contributed to the acceleration of re-epithelialization and the formation of granulation tissue by inducing tumor necrosis factor-α, interleukin-6, basic fibroblast growth factor, transforming growth factor (TGF)-β1, and vascular endothelial growth factor production. In addition, heat-killed KH2 also improved wound closure, which was accompanied by the increased production of TGF-β1 in diabetic mice. Topical administration of heat-killed KH2 might have therapeutic potential for the treatment of chronic skin wounds in diabetes mellitus. In the present study, we concluded that heat-killed KH2 promoted skin wound-healing through the formation of granulation tissues and the production of inflammatory cytokines and growth factors.

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“…Auf volatile Anästhetika wurde in dieser Arbeit nur bei der Instrumentierung zurückgegriffen, sodass präkonditionierende Einflussfaktoren im Rahmen der Ischämie und Reperfusion ausgeschlossen werden konnten. Dass CpG-ODN einen günstigen Einfluss auf Fibrose und Kollagenablagerung hat, wurde bereits in Studien anderer Organsysteme nachgewiesen (Jain et al, 2002;Li et al, 2018;Zhang et al, 2018), die vorliegende Arbeit zeigt erstmals den Einfluss auf die kardiale Infarktnarbe nach Ischämie und Reperfusion. Eindämmung der Infarktexpansion zum Ziel hat (Frangogiannis et al, 2005;Sezaki et al, 2005).…”

Section: Tissue Inhibitor Of Metalloproteinaseunclassified

CpG postconditioning after reperfused myocardial infarction is associated with modulated inflammation, less apoptosis, and better left ventricular function

Duerr¹,

Wu²,

Schneider³

et al. 2020

American Journal of Physiology-Heart and Circulatory Physiology

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Objectives: Postconditioning attenuates inflammation and fibrosis in myocardial infarction (MI). Aim of this study was to investigate whether postconditioning with the CpG-containing TLR9-ligand 1668-thioate (CpG) can modulate inflammation and remodeling in reperfused murine MI. Methods: Thirty min. LAD-occlusion was conducted in 12 weeks old C57BL/6 mice. Mice were treated with CpG i.p. 5 min. before reperfusion. Control group received PBS; sham group did not undergo ischemia. M-mode echocardiography (3, 7 and 28 days) and Millar® left ventricular (LV) catheter were performed (7 and 28 days) before hearts where excised and harvested for immunohistochemical (6, 24 hrs, 3, 7, 28 days), gene expression (6, 24 hrs, 3 days; Taqman® RT-qPCR), protein and FACS analysis (24 hrs, 3 days). Results: Mice treated with CpG showed significantly better LV function after 7- and 28-days reperfusion. Protein- and mRNA-expression of pro- and anti-inflammatory cytokines were significantly induced after CpG-treatment. Histology revealed fewer macrophages in CpG-mice after 24 hrs, confirmed by FACS-analysis with decrease in both, classically M1- and alternative M2a-monocytes. CpG-treatment reduced apoptosis and cardiomyocyte-loss and was associated with induction of adaptive mechanisms, e.g. of heme-oxigenase-1 and b-/a-MHC ratio. Pro-fibrotic markers col-Ia and -III induction was abrogated in CpG-mice, accompanied by fewer myofibroblasts. This lead to formation of a smaller scar. Differential MMP/TIMP-expression contributed to attenuated remodeling in CpG, resulting in preserved cardiac function in a TLR1 and -9 dependant manner. Conclusion: Our study suggests a cardioprotective mechanism of CpG-postconditioning, involving TLR-driven modulation of inflammation. This is followed by attenuated remodeling and preserved LV-function.

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A C‐type CpG ODN accelerates wound healing via regulating fibroblasts and immune response

Cited by 9 publications

References 24 publications

Modulation of immune response in canine chronic wound stimulated with Class C CpG oligonucleotide

Modulation of immune response in canine chronic wound stimulated with Class C CpG oligonucleotide

Topical Administration of Heat-Killed Enterococcus faecalis Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing

CpG postconditioning after reperfused myocardial infarction is associated with modulated inflammation, less apoptosis, and better left ventricular function

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