A/C magnetic hyperthermia of melanoma mediated by iron(0)/iron oxide core/shell magnetic nanoparticles: a mouse study

Balivada, Sivasai; Rachakatla, Raja Shekar; Wang, Hongwang; Samarakoon, Thilani; Dani, Raj Kumar; Pyle, Marla; Kroh, Franklin O.; Walker, Brandon; Leaym, Xiaoxuan; Koper, Olga B.; Tamura, Masaaki; Chikán, Viktor; Bossmann, Stefan H.; Troyer, Deryl L.

doi:10.1186/1471-2407-10-119

Cited by 199 publications

(126 citation statements)

References 23 publications

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“…22,23 To evaluate the efficacy of hyperthermia mediated by Dex-LSMO nanoparticles, we selected murine melanoma (B16F1) cells as a cancer model, since this cancer is reported to be resistant to conventional treatment such as chemotherapy. 24 Before treatment with hyperthermia, it was important to confirm that the Dex-LSMO nanoparticles themselves do not damage cells. We therefore evaluated the cytotoxicity of Dex-LSMO nanoparticles alone in various cell lines.…”

Section: Introductionmentioning

confidence: 99%

Temperature-dependent and time-dependent effects of hyperthermia mediated by dextran-coated La0.7Sr0.3MnO3: in vitro studies

Haghniaz¹,

Umrani²,

Paknikar³

2015

IJN

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Background The purpose of this study was to investigate the therapeutic efficacy of dextran-coated (Dex) La 0.7 Sr 0.3 MnO 3 (LSMO) nanoparticles-mediated hyperthermia at different temperatures (43°C, 45°C, and 47°C) based on cell killing potential and induction of heat shock proteins in a murine melanoma cell (B16F1) line. Methods LSMO nanoparticles were synthesized by a citrate-gel method and coated with dextran. B16F1 cells were exposed to the Dex-LSMO nanoparticles and heated using a radiofrequency generator. After heating, the morphology and topology of the cells were investigated by optical microscopy and atomic force microscopy. At 0 hours and 24 hours post heating, cells were harvested and viability was analyzed by the Trypan blue dye exclusion method. Apoptosis and DNA fragmentation were assessed by terminal deoxynucleotidyl transferase-dUTP nick end labeling (TUNEL) assay and agarose gel electrophoresis, respectively. An enzyme-linked immunosorbent assay was used to quantify heat shock protein levels. Results Our data indicate that cell death and induction of heat shock proteins in melanoma cells increased in a time-dependent and temperature-dependent manner, particularly at temperatures higher than 43°C. The mode of cell death was found to be apoptotic, as evident by DNA fragmentation and TUNEL signal. A minimum temperature of 45°C was required to irreversibly alter cell morphology, significantly reduce cell viability, and result in 98% apoptosis. Repeated cycles of hyperthermia could induce higher levels of heat shock proteins (more favorable for antitumor activity) when compared with a single cycle. Conclusion Our findings indicate a potential use for Dex-LSMO-mediated hyperthermia in the treatment of melanoma and other types of cancer.

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Section: Introductionmentioning

confidence: 99%

Temperature-dependent and time-dependent effects of hyperthermia mediated by dextran-coated La0.7Sr0.3MnO3: in vitro studies

Haghniaz¹,

Umrani²,

Paknikar³

2015

IJN

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“…Previous attempts to implement IV MNPs followed by AMF heating showed some efficacy but were not able to fully ablate tumors, as the required concentration was not reached in the tumors. [57][58][59] From calculations, test tube experiments, and in vitro cell hyperthermia, it appears that ∼0.1%-0.4% iron by weight is required for adequate heating in a tumor. 60,61 A barrier to this approach has been the toxicity of the MNPs at a level that achieves the required tumor loading after IV injection.…”

Section: Introductionmentioning

confidence: 99%

Intravenous magnetic nanoparticle cancer hyperthermia

Huang¹,

Hainfeld²

2013

IJN

104

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Magnetic nanoparticles heated by an alternating magnetic field could be used to treat cancers, either alone or in combination with radiotherapy or chemotherapy. However, direct intratumoral injections suffer from tumor incongruence and invasiveness, typically leaving undertreated regions, which lead to cancer regrowth. Intravenous injection more faithfully loads tumors, but, so far, it has been difficult achieving the necessary concentration in tumors before systemic toxicity occurs. Here, we describe use of a magnetic nanoparticle that, with a well-tolerated intravenous dose, achieved a tumor concentration of 1.9 mg Fe/g tumor in a subcutaneous squamous cell carcinoma mouse model, with a tumor to non-tumor ratio . 16. With an applied field of 38 kA/m at 980 kHz, tumors could be heated to 60°C in 2 minutes, durably ablating them with millimeter (mm) precision, leaving surrounding tissue intact.

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“…These results demonstrate that the antitumor effectiveness of magnetohyperthermia treatment can be associated with cell death in the target tissue and is consistent with the tumor regression observed in hamsters treated with this magnetic fluid composition and AMF. The treatment of tumors via magnetic heating has already demonstrated promising results in other tumors [16,[46][47][48][49].…”

Section: Discussionmentioning

confidence: 99%

High Efficacy in Hyperthermia-associated with Polyphosphate Magnetic Nanoparticles for Oral Cancer Treatment

Taboga¹

2014

J Nanomed Nanotechnol

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Nanotherapy applied to cancer treatment is constantly evolving, and new approaches to current techniques, such as magnetohyperthermia, are being implemented to solve and minimize the limitations of conventional therapeutic strategies. The purpose of this study was to investigate the action of polyphosphate-coated maghemite nanoparticles (MNPs) on oral squamous cell carcinoma. Human oral cancer cells (UM-SCC14A) were incubated with MNPs at various concentrations and subjected to cell proliferation tests (MTT), apoptosis assays and transmission electron image analysis. Viability and apoptotic events were time and dose dependent. These in vitro tests showed that at the intermediate concentration tested there is no significant toxicity, as confirmed by transmission electron microscopy. For this reason this MNPs concentration was chosen for the subsequent in vivo tests. Oral tumor induction was performed by applying the carcinogen DMBA to Syrian hamsters. Animals were then treated by magnetohyperthermia using MNPs. No signs of general clinical symptoms of toxicity or abnormal behavioral reactions were observed. However, animals treated with MNPs and exposed to the alternating magnetic field in the hyperthermia procedure exhibited a significant and time dependent cancer regression, as confirmed by histopathological analyses and immunohistochemistry. Actually, in quantitative terms of the magnetotherapy efficacy involving these polyphosphate-coated MNPs, 100% recovery (12/12) was observed in the oral cancer tumor bearing Syrian hamsters seven days after the treatment with the magnetohyperthermia procedure. Data supports the suggestion that the MNPs-mediated hyperthermia represents a promising strategy for the treatment of oral cancer.in patients with malignancies in the mouth region. Unlike many other oncology subspecialties, however, the rates of recurrence and overall survival have improved only modestly over the past decades, despite the continuous search for new therapeutic techniques [6,7]. Thus, it is expected that the identification and use of specific agents will lead to individualized treatments, which in turn will lead to significantly better results as manifested by more cures and better quality of life by decreasing toxicity [8].Over the last years, maghemite nanoparticles (MNPs) have appeared as a well-established technology and an important field of research in superparamagnetism of colloidal materials, characteristic which allows them to be guided with an external magnetic field [9]. Recently, MNPs have been investigated for therapeutic purposes such as hyperthermic treatment [10,11]. Magnetohyperthermia (MHT) represents a novel and promising therapy for cancer treatment [11,12], being capable of promoting the specific lysis of tumor cells, thereby improving patient outcomes while minimizing the subsequent toxicity effects [13]. The Citation: Candido NM, Calmon MF, Taboga SR, Bonilha JL, dos Santos MC, et al. (2014) High Efficacy in Hyperthermia-associated with Polyphosphate

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A/C magnetic hyperthermia of melanoma mediated by iron(0)/iron oxide core/shell magnetic nanoparticles: a mouse study

Cited by 199 publications

References 23 publications

Temperature-dependent and time-dependent effects of hyperthermia mediated by dextran-coated La0.7Sr0.3MnO3: in vitro studies

Temperature-dependent and time-dependent effects of hyperthermia mediated by dextran-coated La0.7Sr0.3MnO3: in vitro studies

Intravenous magnetic nanoparticle cancer hyperthermia

High Efficacy in Hyperthermia-associated with Polyphosphate Magnetic Nanoparticles for Oral Cancer Treatment

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