A bulk segregant approach to identify genetic polymorphisms associated with cold tolerance in lucerne.

Castonguay, Yves; Cloutier, Jasmin; Laberge, Serge; Bertrand, Alain; Michaud, R.; Chen, T. H. H.; Uemura, Masato; Fujikawa, Seizo

doi:10.1079/9780851990590.0088

Cited by 2 publications

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“…Although extensive research has been conducted on winter hardiness worldwide, most focused on the physiological indicators of winter hardiness for alfalfa [13][14][15]. Investigations on morphological indicators are relatively limited [16,17]. To date, a unified winter hardiness grading standard has not been established to measure alfalfa's ability to survive the winter and regenerate in spring [18] or, in other words, its performance to tolerate frost, snow accumulation, freezing, drying and other extreme winter stresses [12,19].…”

Section: Introductionmentioning

confidence: 99%

A Multi-Site Evaluation of Winter Hardiness in Indigenous Alfalfa Cultivars in Northern China

Liu

Nie

et al. 2021

Atmosphere

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Integration of perennial grass species into the current food production systems, especially in the agropastoral regions worldwide, may produce multiple benefits including, among others, a more stable productivity and a smaller eco-environmental footprint. However, one of the fundamental challenges facing the large-scale adoption of such grass species is their ability to withstand the vagaries of winter in these regions. Here, we present a comprehensive evaluation of the winter hardiness of 50 indigenous Chinese cultivars of alfalfa, a high-quality leguminous perennial grass, in comparison with six introduced U.S. cultivars in a multi-site field experiment in northern China. Our results reveal that indigenous cultivars have stronger winter hardiness than introduced cultivars. Cultivars native in the north performed better than southern cultivars, suggesting that suitability evaluation is an unavoidable step proceeding any regional implementations. Our results also show that the metric we used to assess alfalfa’s winter hardiness, the average score index (ASI), produced more consistent results than another more-widely used metric of winter survival rate (WSR). These findings offer a systematic field evidence that supports regional cropping system adjustment and production system betterment to ensure food security under climate change in the region and beyond.

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Section: Introductionmentioning

confidence: 99%

A Multi-Site Evaluation of Winter Hardiness in Indigenous Alfalfa Cultivars in Northern China

Liu

Nie

et al. 2021

Atmosphere

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“…frost or drought) is based on the assessment of shifts in marker allele frequency from unselected to selected material through a bulk segregant analysis (Skinner et al 2000;Castonguay et al 2006). Its efficiency is expected to increase if the stress application can be controlled, the phenotypic selection of individual plants is performed through procedures which minimize the experimental error (e.g.…”

Section: Which Opportunities For Marker-assisted Selection?mentioning

confidence: 99%

Questions and avenues for lucerne improvement

Annicchiarico¹,

Scotti²,

Carelli³

et al. 2010

CZECH J GENET PLANT

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Six crucial questions for lucerne breeders are set up and discussed in relation to the available information. (i) Which width of adaptation? Genotype × location interaction is region-specific and may be wide enough to justify breeding for specific adaptation. Genotype × exploitation interaction requires contrasting plant types for mowing and intensive grazing. (ii) Can we breed very drought-tolerant varieties? One drought-tolerant landrace exhibited a drought-avoidance, water-conservation strategy based on limited root development, while large root featured material adapted to favourable environments and/or frequent mowing. (iii) Which selection scheme and variety type? Many schemes were proposed for synthetic varieties, but empirical or theoretical comparisons were limited in number and inference space. Non-additive genetic variation may be exploited by free hybrids (semi-hybrids) through procedures varying in complexity, possibly assisted by marker evaluation. Previous selection of exotic germplasm for adaptation is essential. (iv) How to improve the forage quality? Selection for modified stem morphology (increased internode number, decreased internode length) proved effective. Combined selection for forage yield and leaf/stem ratio seems also feasible. (v) Which opportunities for marker-assisted selection? Linkage maps for lucerne are available but useful markers for forage yield may be site-specific. Bulk segregant analysis is promising in breeding for stress tolerance. (vi) How to exploit genomic information from M. truncatula? This model species can help in developing markers and locating genes which control metabolic pathways, such as saponin content and composition. Information from M. truncatula on marker-trait association for forage yield or tolerance to abiotic stresses may be little exploitable.

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A bulk segregant approach to identify genetic polymorphisms associated with cold tolerance in lucerne.

Cited by 2 publications

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A Multi-Site Evaluation of Winter Hardiness in Indigenous Alfalfa Cultivars in Northern China

A Multi-Site Evaluation of Winter Hardiness in Indigenous Alfalfa Cultivars in Northern China

Questions and avenues for lucerne improvement

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