A BTB/POZ domain-containing protein negatively regulates plant immunity in Nicotiana benthamiana

Zhao, Ming; Yu, Gui; Xu, Zhangyan; Ouyang, Xue; Jia, Yuling; Liu, Jiangtao; Zhang, Meixiang; An, Yuyan

doi:10.1016/j.bbrc.2022.02.050

Cited by 12 publications

(10 citation statements)

References 36 publications

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“…Bacterial physiological activities, such as virulence and pathogenesis, play pivotal roles in R. solanacearum infection. , Previous studies have reported a strong correlation between the expression of pathogenicity-related genes and colonization. , Especially, the type III secretion system with its delivered type III effectors is the main virulence determinants of R. solanacearum, and more than 100 different type III effectors have been identified .…”

Section: Discussionmentioning

confidence: 99%

Efficacy of 2,4-Di-tert-butylphenol in Reducing Ralstonia solanacearum Virulence: Insights into the Underlying Mechanisms

Yang,

Wang,

Lin

et al. 2024

ACS Omega

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Ralstonia solanacearum can induce severe wilt disease in vital crops. Therefore, there is an urgent need to develop novel antifungal solutions. The natural compound 2,4-di-tert-butylphenol (2,4-DTBP) exhibits diverse physiological activities and affects soil function. However, its specific impact on the R. solanacearum remains unclear. Here, we investigated the antimicrobial potential of 2,4-DTBP. The results demonstrated that 2,4-DTBP effectively inhibited its growth and altered morphology. In addition, it substantially impeded biofilm formation, motility, and exopolysaccharide secretion. Transcriptomic analysis revealed that 2,4-DTBP inhibited energy production and membrane transport. Additionally, 2,4-DTBP hindered the growth by interfering with the membrane permeability, reactive oxygen species (ROS) production, and electrolyte leakage. Concomitantly, this led to a significant reduction in pathogenicity, as evidenced by the biomass of R. solanacearum in the invaded roots. Overall, our data strongly support the potential utility of 2,4-DTBP as a potent antibacterial agent capable of effectively preventing the onset of bacterial wilt caused by R. solanacearum.

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Section: Discussionmentioning

confidence: 99%

Efficacy of 2,4-Di-tert-butylphenol in Reducing Ralstonia solanacearum Virulence: Insights into the Underlying Mechanisms

Yang,

Wang,

Lin

et al. 2024

ACS Omega

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“…Interestingly, they can stimulate but also repress the plant immune system (Orosa et al, 2017). For instance, it has been shown in Nicotiana benthamiana that the BTB/POZ complex NbBTB negatively regulates plant resistance against Phytophthora parasitica and it is easily triggered by pathogen effectors (Zhao et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Genetic mapping of the root-mycobiota in rice and its role in drought tolerance

Andreo-Jiménez

Beest

Kruijer

et al. 2023

Preprint

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Background Rice is the second most produced crop worldwide, but it is highly susceptible to drought-induced yield loss. Micro-organisms can potentially alleviate the negative effects of drought but how they are associated with their host is unclear. The aim of the present study was to unravel the genetic factors involved in this process, and whether it plays a role in rice drought tolerance. Results To achieve this, we characterized the composition of the root-mycobiota in 296 rice accessions (Oryza sativaL. subsp. indica), grown in the field under control and drought conditions. A Genome Wide Association Study (GWAS) was used to identify marker-trait associations between rice Single Nucleotide Polymorphism (SNP) markers and root mycobiota. This resulted in the identification of ten significant (LOD > 4) SNPs associated with six root associated fungi and four significant SNPs associated with fungi-mediated drought tolerance. The analysis of the genes underlying the loci of the significant marker-trait associations identified a priori candidates including genes known to be involved in pathogen defense, abiotic stress responses and cell-wall remodeling processes. Conclusion Our study shows that the rice genetics affects the recruitment of fungi, some of which affect yield under drought, and provides candidate gene targets for breeding to improve rice-fungal interactions and drought tolerance.

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“…Interestingly, BTB/POZ can stimulate but also repress the plant immune system (Orosa et al 2017 ). For instance, in Nicotiana benthamiana, the BTB/POZ complex NbBTB has been shown to negatively regulate plant resistance against Phytophthora parasitica and is easily triggered by pathogen effectors (Zhao et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic Mapping of the Root Mycobiota in Rice and its Role in Drought Tolerance

Andreo-Jiménez

Beest

Kruijer

et al. 2023

Rice

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Background Rice is the second most produced crop worldwide, but is highly susceptible to drought. Micro-organisms can potentially alleviate the effects of drought. The aim of the present study was to unravel the genetic factors involved in the rice-microbe interaction, and whether genetics play a role in rice drought tolerance. For this purpose, the composition of the root mycobiota was characterized in 296 rice accessions (Oryza sativa L. subsp. indica) under control and drought conditions. Genome wide association mapping (GWAS) resulted in the identification of ten significant (LOD > 4) single nucleotide polymorphisms (SNPs) associated with six root-associated fungi: Ceratosphaeria spp., Cladosporium spp., Boudiera spp., Chaetomium spp., and with a few fungi from the Rhizophydiales order. Four SNPs associated with fungi-mediated drought tolerance were also found. Genes located around those SNPs, such as a DEFENSIN-LIKE (DEFL) protein, EXOCYST TETHERING COMPLEX (EXO70), RAPID ALKALINIZATION FACTOR-LIKE (RALFL) protein, peroxidase and xylosyltransferase, have been shown to be involved in pathogen defense, abiotic stress responses and cell wall remodeling processes. Our study shows that rice genetics affects the recruitment of fungi, and that some fungi affect yield under drought. We identified candidate target genes for breeding to improve rice-fungal interactions and hence drought tolerance.

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A BTB/POZ domain-containing protein negatively regulates plant immunity in Nicotiana benthamiana

Cited by 12 publications

References 36 publications

Efficacy of 2,4-Di-tert-butylphenol in Reducing Ralstonia solanacearum Virulence: Insights into the Underlying Mechanisms

Efficacy of 2,4-Di-tert-butylphenol in Reducing Ralstonia solanacearum Virulence: Insights into the Underlying Mechanisms

Genetic mapping of the root-mycobiota in rice and its role in drought tolerance

Genetic Mapping of the Root Mycobiota in Rice and its Role in Drought Tolerance

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