A Broad Application of CRISPR Cas9 in Infectious Diseases of Central Nervous System

Bellizzi, Anna; Ahye, Nicholas; Jalagadugula, Gauthami; Wollebo, Hassen S.

doi:10.1007/s11481-019-09878-7

Cited by 5 publications

(4 citation statements)

References 202 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…При этом стоит отметить ответную приспособительную способность вирусов к применяемым методам лечения. Несмотря на усовершенствование инвазивных свойств препаратов для преодоления биологических барьеров, развитие заболевания имеет длительное течение, нередко приводящее к гибели реципиента, что показательно для семейств Retroviridae, Herpesviridae и Polyomaviridae [2]. Несмотря на то, что различные виды вирусов имеют разные пути доступа к центральной нервной системе (ЦНС), вирусные инфекции отличаются от других микроорганизмов тем обстоятельством, что в начальной стадии они персистируют на периферии и таким образом получают доступ к структурам ЦНС.…”

Section: раздел 1 введениеunclassified

“…Сложно передать, насколько CRISPR революционна, но она имеет огромный потенциал, способный навсегда изменить диагностику и терапию многочисленных заболеваний человечества. Эммануэль Шарпантье и Дженнифер Дудна (2020) получили Нобелевскую премию в области химии за развитие метода редактирования генома -технологию CRISPR-Cas9 [2,3].…”

Section: раздел 1 введениеunclassified

See 1 more Smart Citation

Prospects for using CRISPR-Cas9 system in the treatment of human viral diseases

Зиганшин

Mulyukov

Omarov

et al. 2023

Acta biomedica scientifica

View full text Add to dashboard Cite

The aim. To analyze the possibility of using the genetic mechanisms of CRISPR-Cas9 technology in the prevention and treatment of certain viral diseases.Materials and methods. The search for publications was carried out in Russian and foreign literature using the following search engines: RSCI, Cyberleninka, eLibrary, PubMed, Cochrane Library, etc. A review of domestic and international scientific papers on the research topic was carried out using search keywords: CRISPR, genetic engineering, genome editing, Cas9, sgRNA.Results. A review of using CRISPR-Cas9 method (“genetic scissors”) as a gene therapy for some viral diseases was carried out, and its main advantages and disadvantages were revealed. An analysis of the data of scientific studies on genetic research methods over the past decade discovers the main aspects of CRISPR-Cas9 technology, modern classification and prospects for using this technology in clinical practice for the treatment and prevention of human viral diseases. The possibilities of creating a more versatile and stable version of the CRISPR-Cas9 technology are considered. Particular attention is paid to the technological difficulties and obstacles that scientists face when implementing this system for targeted use in clinical medicine.Conclusion. One of the rapidly developing areas in science giving promising prospects for modern healthcare is genetic engineering, especially in cases where scientific developments are applied in clinical practice. The discovery of “genetic scissors” technology has revolutionized all medicine. Wide opportunities for developing new treatment methods for many viral diseases and creating conditions for their early prevention opened up for the medical community. In the future, with the introduction of this technology into clinical practice, it will become possible to treat diseases that have not previously responded to ongoing therapy and were considered incurable.

show abstract

Section: раздел 1 введениеunclassified

Prospects for using CRISPR-Cas9 system in the treatment of human viral diseases

Зиганшин

Mulyukov

Omarov

et al. 2023

Acta biomedica scientifica

View full text Add to dashboard Cite

show abstract

“…A more recent development is the emerging technology associated with CRISPR-based gene editing strategies to identify causal mechanisms of viral replication and virus-mediated pathology. Targeted editing of viruses via CRISPR/Cas9 may provide a new therapeutic approach to eliminate viruses in various states of latency within the CNS ( 179 ). This approach can also be used to identify cellular components within the host that permit viral infections ( 180 , 181 ).…”

Section: Two-dimensional Culturesmentioning

confidence: 99%

Using 2D and 3D pluripotent stem cell models to study neurotropic viruses

2022

View full text Add to dashboard Cite

Understanding the impact of viral pathogens on the human central nervous system (CNS) has been challenging due to the lack of viable human CNS models for controlled experiments to determine the causal factors underlying pathogenesis. Human embryonic stem cells (ESCs) and, more recently, cellular reprogramming of adult somatic cells to generate human induced pluripotent stem cells (iPSCs) provide opportunities for directed differentiation to neural cells that can be used to evaluate the impact of known and emerging viruses on neural cell types. Pluripotent stem cells (PSCs) can be induced to neural lineages in either two- (2D) or three-dimensional (3D) cultures, each bearing distinct advantages and limitations for modeling viral pathogenesis and evaluating effective therapeutics. Here we review the current state of technology in stem cell-based modeling of the CNS and how these models can be used to determine viral tropism and identify cellular phenotypes to investigate virus-host interactions and facilitate drug screening. We focus on several viruses (e.g., human immunodeficiency virus (HIV), herpes simplex virus (HSV), Zika virus (ZIKV), human cytomegalovirus (HCMV), SARS-CoV-2, West Nile virus (WNV)) to illustrate key advantages, as well as challenges, of PSC-based models. We also discuss how human PSC-based models can be used to evaluate the safety and efficacy of therapeutic drugs by generating data that are complementary to existing preclinical models. Ultimately, these efforts could facilitate the movement towards personalized medicine and provide patients and physicians with an additional source of information to consider when evaluating available treatment strategies.

show abstract

“…The CRISPR/Cas9 off-target concerns pose challenges for research advancements and therapeutic utilizations. Therefore, researchers have developed methods such as advanced versions or Cas9 nickases to minimize off-target activities (Bellizzi et al, 2019) and cytotoxicity (Wang et al, 2016). The two parts of CRISPR/Cas9 system, the optimizations of Cas9 proteins or the upgradations of gRNA, have equally contributed to the reductions in CRISPR/Cas9 off-target effects.…”

Section: Crispr/cas9 Off-target Effectsmentioning

confidence: 99%

The Use of CRISPR/Cas9 as a Tool to Study Human Infectious Viruses

Lin

Peng

et al. 2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Clustered regularly interspaced short palindromic repeats (CRISPR) systems are a set of versatile gene-editing toolkit that perform diverse revolutionary functions in various fields of application such as agricultural practices, food industry, biotechnology, biomedicine, and clinical research. Specially, as a novel antiviral method of choice, CRISPR/Cas9 system has been extensively and effectively exploited to fight against human infectious viruses. Infectious diseases including human immunodeficiency virus (HIV), hepatitis B virus (HBV), human papillomavirus (HPV), and other viruses are still global threats with persistent potential to probably cause pandemics. To facilitate virus removals, the CRISPR/Cas9 system has already been customized to confer new antiviral capabilities into host animals either by modifying host genome or by directly targeting viral inherent factors in the form of DNA. Although several limitations and difficulties still need to be conquered, this technology holds great promises in the treatment of human viral infectious diseases. In this review, we will first present a brief biological feature of CRISPR/Cas9 systems, which includes a description of CRISPR/Cas9 structure and composition; thereafter, we will focus on the investigations and applications that employ CRISPR/Cas9 system to combat several human infectious viruses and discuss challenges and future perspectives of using this new platform in the preclinical and clinical settings as an antiviral strategy.

show abstract

A Broad Application of CRISPR Cas9 in Infectious Diseases of Central Nervous System

Cited by 5 publications

References 202 publications

Prospects for using CRISPR-Cas9 system in the treatment of human viral diseases

Prospects for using CRISPR-Cas9 system in the treatment of human viral diseases

Using 2D and 3D pluripotent stem cell models to study neurotropic viruses

The Use of CRISPR/Cas9 as a Tool to Study Human Infectious Viruses

Contact Info

Product

Resources

About