A bright and colorful future for <scp>DNA</scp> cell cycle analysis

Yuan, Constance

doi:10.1002/cyto.a.22821

Cited by 2 publications

(4 citation statements)

References 15 publications

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“…Ploidy level determination is frequently applied in the evaluation of breast, prostatic, or bladder carcinomas. Ploidy level is also an important prognostic factor of hematolymphoid malignancies (e.g., B‐lymphoblastic leukemia and plasma cell myeloma) (6).…”

Section: Dye Excitation Emission Membrane Permeability Dna Sequence Preferencementioning

confidence: 99%

Can Flow Cytometric DNA Content Analysis Become a Routine Procedure in Aquaculture?

Krasznai

Goda

2020

Cytometry Pt A

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Section: Dye Excitation Emission Membrane Permeability Dna Sequence Preferencementioning

confidence: 99%

Can Flow Cytometric DNA Content Analysis Become a Routine Procedure in Aquaculture?

Krasznai

Goda

2020

Cytometry Pt A

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“…Risk stratification and prognostication in haemato-lymphoid neoplasms is commonly performed using karyotyping, fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR). In some countries, single nucleotide polymorphism (SNP) arrays and array comparative genomic hybridization (aCGH) are utilized however these are not cost-effective in limited resources settings 1 - 5 .…”

Section: Introductionmentioning

confidence: 99%

“…Hence, only DNA analysis is not sufficient on its own in specifically delineating the tumor population and requires concurrent surface immunophenotyping 5 , 10 - 12 . Over the last decade, clinical flow cytometers have undergone metamorphosis, with availability of tandem dyes and multiple lasers, permitting up to 8-10 colour flowcytometry along with development of new reagents like FxCycle TM violet (FCV) 1 , 5 , 13 .…”

Section: Introductionmentioning

confidence: 99%

“…FCV (4’,6-diamidino-2-phenylindole, dihydrochloride) from Invitrogen, is a cell-impermeant DNA selective dye that is excited with 405/407 nm laser (violet laser) with emission collected in the 450/50 band pass filter or equivalent. This spares the spectrum of colours excited with blue as well as red laser for simultaneous 6-7 colour immunophenotyping, and requires only a single step modification of the routinely used stain —› lyse —› wash protocol 1 , 5 , 13 .…”

Section: Introductionmentioning

confidence: 99%

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Flow Cytometric DNA Ploidy Analysis in Haemato-Lymphoid Neoplasms: An Analysis of 132 Cases

Gupta¹,

Mittal²,

Dadu³

et al. 2022

IJHOSCR

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Background: FxCycleTM Violet (FCV) based flow cytometric (FCM) DNA ploidy analysis is a rapid and simple tool that can substantiate in characterizing the biological behaviour across the spectrum of haematological malignancies and correlates with cytogenetic studies. Materials and Methods: In this prospective study, we performed simultaneous immunophenotyping with FCV based on ploidy analysis in n=132 consecutive new samples, comprising n=110 samples of haemato-lymphoid neoplasms, including acute leukemias (n=67, 60.9%), CML with myeloid blast crisis (n=1, 0.9%), MDS with excess blasts (n=2, 1.8%), mature B cell/ T cell neoplasms (n=37, 33.7%), multiple myeloma (n=3, 2.7%) along with n=22 normal samples. The FCM DNA data was compared with corresponding conventional karyotyping results, wherever available. Results: In FCM ploidy analysis (n=110), the overall DNA index (DI) ranged from 0.81 to 2.17 and S-Phase fraction (SPF) from 0.1-31.6%. Diploidy was seen in n = 90 (81.8%), low-hyperdiploidy in n = 10 (9.1%), high-hyperdiploidy in n = 7 (6.4%) with one case each (0.9% each) having near-tetraploidy, high-hypodiploidy and low-hypodiploidy. The DI of all viable cell populations in normal samples ranged from 0.96-1.05. Conventional karyotyping was performed in n=76/110 cases (70%) with n= 11/76 (15%) culture failures. The modal chromosome number ranged from 45 to 63. A concordance of 95.4% (n=62/65) was noted with corresponding FCM DI. Conclusion: FCV-based ploidy is a sensitive technique that provides complementary information and ascertains a strong correlation with conventional cytogenetics across all haemato-lymphoid neoplasms. It can detect aneuploidy in all B-ALL and myeloma cases, even in hemodiluted samples with cytogenetic culture failure; supplement the diagnoses of erythroleukemia, and provide a useful screen for a higher grade lymph node disease in lymphoma cases with SPF > 3%.

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A bright and colorful future for DNA cell cycle analysis

Cited by 2 publications

References 15 publications

Can Flow Cytometric DNA Content Analysis Become a Routine Procedure in Aquaculture?

Can Flow Cytometric DNA Content Analysis Become a Routine Procedure in Aquaculture?

Flow Cytometric DNA Ploidy Analysis in Haemato-Lymphoid Neoplasms: An Analysis of 132 Cases

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