A Brief Review of Molecular Techniques to Assess Plant Diversity

Arif, Ibrahim A.; Bakir, Mohammad Abdul; Khan, Haseeb A.; Farhan, Ahmad H. Al; Homaidan, Ali A. Al; Bahkali, Ali H.; Sadoon, Mohammad Al; Shobrak, Mohammad

doi:10.3390/ijms11052079

Cited by 144 publications

(83 citation statements)

References 86 publications

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“…The impact of this erosion in plant biodiversity has created environmental and socioeconomic problems which subsequently triggered the need of conservation of plant resources (Arif et al, 2010). According to Tourenq (2008) drought is the main threat facing biodiversity along with coastal development and urbanization and overexploitation of natural resources (grazing, fishing, hunting, and water extraction).…”

Section: Threats To Uae Biodiversitymentioning

confidence: 99%

Landscaping with native plants in the UAE: A review

et al. 2017

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The United Arab Emirates (UAE) has a unique experience in greening the desert. Huge efforts have been done to create green spaces across the country including afforestation and urban landscaping. Most of the landscaping designs were inspired from western landscapes comprises mostly of exotic species, which do not reconcile with the environmental conditions of the UAE. The intensive use of exotic species in artificial vegetation, high water requirements for the UAE greenery, coupled with water shortages and salinity, are causing major environmental and ecological challenges. Fortunately, the UAE hosts a unique flora and fauna that show remarkable adjustment to harsh weather conditions. Here we emphasize the use of native plants due to their potential to develop landscapes in saline and water shortage conditions, leading to reduced water usage for landscaping. The preservation of native biodiversity of the UAE will be an added benefit. In this article the main aspects of the UAE landscaping efforts, with the associated water resources using native plants in landscaping, problems in promoting native plants in landscaping and possible solutions are discussed.

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Section: Threats To Uae Biodiversitymentioning

confidence: 99%

Landscaping with native plants in the UAE: A review

et al. 2017

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“…The RAPD markers have been independently developed by Willians et al (1990) and Welsh e McClelland (1990). The use of the marker in plant diversity has been reviewed by Arif et al (2010).…”

Section: Random Amplified Dna Reveals Polymorphismmentioning

confidence: 99%

Biodiversity Measures in Agriculture Using DNA

Hoffmann¹,

Borba²,

Allem³

et al. 2011

Biodiversity

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“…These primers detect polymorphisms in the absence of specific DNA sequences, and the polymorphisms function as genetic markers that can be used to construct genetic maps (Arif et al, 2010). The RAPD technique requires low concentrations of template DNA without any hazardous contamination, and is simple and inexpensive.…”

Section: Introductionmentioning

confidence: 99%

An improved DNA marker technique for genetic characterization using RAMP-PCR with high-GC primers

et al. 2016

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ABSTRACT. Random amplified polymorphic DNA (RAPD) is a widely used molecular marker technique. As traditional RAPD has poor reproducibility and productivity, we previously developed an improved RAPD method (termed RAMP-PCR), which increased the reproducibility, number of bands, and efficiency of studies on polymorphism. To further develop the efficiency of this method, we used high-GC content primers for improved RAMP-PCR with DNA samples from Lonicera japonica. Comparison of amplification profiles obtained by standard RAPD primers with those obtained by regular PCR and RAMP-PCR, and high-GC primers with regular PCR and RAMP-PCR showed that the average number of bands and polymorphisms per primer gradually and significantly increased (from 6.4 to 15.0 and from 4.6 to 10.2, respectively). Cluster dendrograms showed similar results, indicating that this new method is consistent and reproducible. A total of 22 samples from different species, including plants, animals, and humans, were used for RAMP-PCR with high-GC primers. Multiple bands were successfully amplified from all samples, demonstrating that this method is a reliable technique with consistent results and may be of general interest in studies on different genera and species. We developed highly effective DNA markers, which can provide a more effective and potentially valuable approach than traditional RAPD for the genetic identification of various organisms, particularly of medicinal plants.

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A Brief Review of Molecular Techniques to Assess Plant Diversity

Cited by 144 publications

References 86 publications

Landscaping with native plants in the UAE: A review

Landscaping with native plants in the UAE: A review

Biodiversity Measures in Agriculture Using DNA

An improved DNA marker technique for genetic characterization using RAMP-PCR with high-GC primers

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