2019
DOI: 10.1016/j.mib.2019.10.002
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A brief from the leaf: latest research to inform our understanding of the phyllosphere microbiome

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Cited by 112 publications
(82 citation statements)
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“…We found distinct structural differences between phyllosphere and rhizosphere fungal communities among the different plant growth stages. The potential mechanism for this is the widely accepted assumption that plant leaves phenotypically differ among their different developmental stages, for which a subset of plant leaf migrants or colonizers are favored, consequently contributing to variation in fungal community composition ( Leveau, 2019 ). In this study, phyllosphere and rhizosphere fungal community richness was both significantly higher during the tiller stage ( Figure 1 ).…”
Section: Discussionmentioning
confidence: 99%
“…We found distinct structural differences between phyllosphere and rhizosphere fungal communities among the different plant growth stages. The potential mechanism for this is the widely accepted assumption that plant leaves phenotypically differ among their different developmental stages, for which a subset of plant leaf migrants or colonizers are favored, consequently contributing to variation in fungal community composition ( Leveau, 2019 ). In this study, phyllosphere and rhizosphere fungal community richness was both significantly higher during the tiller stage ( Figure 1 ).…”
Section: Discussionmentioning
confidence: 99%
“…Sonication was used to remove bacteria from the leaf surface in order to minimise undesirable amplification of chloroplast rRNA genes and contamination by endophytic bacteria, which are typically recovered via methods that inflict damage to leaves such as maceration and coring [44,45]. After sonication, the wash buffer was decanted, syringe filtered through 90 μm nylon mesh to remove finer plant debris, and centrifuged (3,200 x g for 30 minutes). The supernatant was discarded, and the bacterial cell pellet was resuspended in 270 μl of PBS, transferred to a 1.5-2.0 mL screw-capped conical bottomed polypropylene tube containing 0.5 g each of 0.1 mm and 2.5 mm silica-zirconia beads, and frozen at -80˚C until further processing.…”
Section: Bacterial Community Collectionmentioning
confidence: 99%
“…The assembly of the phyllosphere microbiome, herein strictly defined as epiphytic bacterial communities on the leaf surface, can be shaped by the microbial communities present in the surrounding environment (i.e., stochastic colonisation) and the host plant (i.e., biotic selection) [3,9]. However, although the leaf surface is generally considered a discrete microbial habitat [10,11], there is no consensus on the dominant driver of community assembly across phyllosphere microbiomes.…”
Section: Introductionmentioning
confidence: 99%
“…Many microorganisms including lamentous fungi, yeast, archaea and algae were found to colonize leaves [5]. So far, the plant phyllosphere was found to be in uenced by a number of different parameters including the plant genotype but also environmental uctuations and macro and micro climatic conditions [6][7][8]. Moreover, the importance of the phyllosphere inhabiting microbiota in the defense of plants against pathogens was shown [8,9].…”
Section: Introductionmentioning
confidence: 99%