A brief from the leaf: latest research to inform our understanding of the phyllosphere microbiome

Leveau, Johan H. J.

doi:10.1016/j.mib.2019.10.002

Cited by 112 publications

(82 citation statements)

References 83 publications

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“…We found distinct structural differences between phyllosphere and rhizosphere fungal communities among the different plant growth stages. The potential mechanism for this is the widely accepted assumption that plant leaves phenotypically differ among their different developmental stages, for which a subset of plant leaf migrants or colonizers are favored, consequently contributing to variation in fungal community composition ( Leveau, 2019 ). In this study, phyllosphere and rhizosphere fungal community richness was both significantly higher during the tiller stage ( Figure 1 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of Plant and Soil Characteristics on Phyllosphere and Rhizosphere Fungal Communities During Plant Development in a Copper Tailings Dam

et al. 2020

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Interactions between plants and microbes can affect ecosystem functions, and many studies have demonstrated that plant properties influence mutualistic microorganisms. Here, high-throughput sequencing was used to investigate rhizosphere and phyllosphere fungal communities during different plant development stages. Results demonstrated that phyllosphere and rhizosphere fungal community structures were distinct during all developmental stages while they were mediated separately by plant carbon and soil sulfur. Comparatively, the effect of root properties on phyllosphere fungal diversity was greater than soil properties. Moreover, rhizosphere fungal networks of Bothriochloa ischaemum were more complex than phyllosphere fungal networks. This study demonstrated that the effect of plant and soil traits on phyllosphere and rhizosphere fungal communities could potentially be significant, depending on the applicable environmental condition and plant development stage. Although links between phyllosphere and rhizosphere communities have been established, further studies on functional fungal groups during phytoremediation processes are necessary. This study comprehensively analyzed dynamic relationships between phyllosphere and rhizosphere fungal communities during different plant development stages in a polluted environment. These fungal communities were determined to be expedient to the development and utilization of beneficial microbial communities during different development stages, which could more effectively help to stabilize and reclaim contaminated copper tailings soil.

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Section: Discussionmentioning

confidence: 99%

Effects of Plant and Soil Characteristics on Phyllosphere and Rhizosphere Fungal Communities During Plant Development in a Copper Tailings Dam

et al. 2020

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“…Sonication was used to remove bacteria from the leaf surface in order to minimise undesirable amplification of chloroplast rRNA genes and contamination by endophytic bacteria, which are typically recovered via methods that inflict damage to leaves such as maceration and coring [44,45]. After sonication, the wash buffer was decanted, syringe filtered through 90 μm nylon mesh to remove finer plant debris, and centrifuged (3,200 x g for 30 minutes). The supernatant was discarded, and the bacterial cell pellet was resuspended in 270 μl of PBS, transferred to a 1.5-2.0 mL screw-capped conical bottomed polypropylene tube containing 0.5 g each of 0.1 mm and 2.5 mm silica-zirconia beads, and frozen at -80˚C until further processing.…”

Section: Bacterial Community Collectionmentioning

confidence: 99%

“…The assembly of the phyllosphere microbiome, herein strictly defined as epiphytic bacterial communities on the leaf surface, can be shaped by the microbial communities present in the surrounding environment (i.e., stochastic colonisation) and the host plant (i.e., biotic selection) [3,9]. However, although the leaf surface is generally considered a discrete microbial habitat [10,11], there is no consensus on the dominant driver of community assembly across phyllosphere microbiomes.…”

Section: Introductionmentioning

confidence: 99%

A core phyllosphere microbiome exists across distant populations of a tree species indigenous to New Zealand

et al. 2020

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The phyllosphere microbiome is increasingly recognised as an influential component of plant physiology, yet it remains unclear whether stable host-microbe associations generally exist in the phyllosphere. Leptospermum scoparium (mānuka) is a tea tree indigenous to New Zealand, and honey derived from mānuka is widely known to possess unique antimicrobial properties. However, the host physiological traits associated with these antimicrobial properties vary widely, and the specific cause of such variation has eluded scientists despite decades of research. Notably, the mānuka phyllosphere microbiome remains uncharacterised, and its potential role in mediating host physiology has not been considered. Working within the prevailing core microbiome conceptual framework, we hypothesise that the phyllosphere microbiome of mānuka exhibits specific host association patterns congruent with those of a microbial community under host selective pressure (null hypothesis: the mānuka phyllosphere microbiome is recruited stochastically from the surrounding environment). To examine our hypothesis, we characterised the phyllosphere and associated soil microbiomes of five distinct and geographically distant mānuka populations across the North Island of New Zealand. We identified a habitat-specific and relatively abundant core microbiome in the mānuka phyllosphere, which was persistent across all samples. In contrast, non-core phyllosphere microorganisms exhibited significant variation across individual host trees and populations that was strongly driven by environmental and spatial factors. Our results demonstrate the existence of a dominant and ubiquitous core microbiome in the phyllosphere of mānuka, supporting our hypothesis that phyllosphere microorganisms of mānuka exhibit specific host association and potentially mediate physiological traits of this nationally and culturally treasured indigenous plant. In addition, our results illustrate biogeographical patterns in mānuka phyllosphere microbiomes and offer insight into factors contributing to phyllosphere microbiome assembly.

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“…Many microorganisms including lamentous fungi, yeast, archaea and algae were found to colonize leaves [5]. So far, the plant phyllosphere was found to be in uenced by a number of different parameters including the plant genotype but also environmental uctuations and macro and micro climatic conditions [6][7][8]. Moreover, the importance of the phyllosphere inhabiting microbiota in the defense of plants against pathogens was shown [8,9].…”

Section: Introductionmentioning

confidence: 99%

Trichomes form genotype-specific microbial hotspots in the phyllosphere of tomato

Kusstatscher

Wicaksono

Bergna

et al. 2020

Preprint

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Abstract Background : The plant phyllosphere is a well-studied habitat characterized by low nutrient availability and high community dynamics. In contrast, plant trichomes, known for their production of a large number of metabolites, are a yet unexplored habitat for microbes. We analyzed the phyllosphere as well as trichomes of two tomato genotypes (Solanum lycopersicum LA4024, S. habrochaites LA1777) by targeting bacterial 16S rRNA gene fragments.Results: Leaves, leaves without trichomes, and trichomes alone harbored similar abundances of bacteria (108- 109 16S rRNA gene copy numbers per gram of sample). In contrast, bacterial diversity was found significantly increased in trichome samples (Shannon index: 4.4 vs. 2.5). Moreover, the community composition was significantly different when assessed with beta diversity analysis and corresponding statistical tests. At the bacterial class level, Alphaproteobacteria (23.6%) were significantly increased, whereas Bacilli (8.6%) were decreased in trichomes. The bacterial family Sphingomonadacea (8.4%) was identified as the most prominent, trichome-specific taxa; Burkholderiaceae and Actinobacteriaceae showed similar pattern. Moreover, Sphingomonas was identified as a central element in the core microbiome of trichome samples, while distinct low-abundant bacterial families including Hymenobacteraceae and Alicyclobacillaceae were exclusively found in trichome samples. Niche preferences were statistically significant for both genotypes and genotype-specific enrichments were further observed.Conclusion : Our results provide first evidence of a highly specific trichome microbiome in tomato and show the importance of micro-niches for the structure of bacterial communities on leaves. These findings provide further clues for breeding, plant pathology and protection as well as so far unexplored natural pathogen defense strategies.

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A brief from the leaf: latest research to inform our understanding of the phyllosphere microbiome

Cited by 112 publications

References 83 publications

Effects of Plant and Soil Characteristics on Phyllosphere and Rhizosphere Fungal Communities During Plant Development in a Copper Tailings Dam

Effects of Plant and Soil Characteristics on Phyllosphere and Rhizosphere Fungal Communities During Plant Development in a Copper Tailings Dam

A core phyllosphere microbiome exists across distant populations of a tree species indigenous to New Zealand

Trichomes form genotype-specific microbial hotspots in the phyllosphere of tomato

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