A bridging SF/Alg composite scaffold loaded NGF for spinal cord injury repair

Jiao, Genlong; Pan, Yongqin; Wang, Cunchuang; Li, ZhaoXia; Li, Zhizhong; Guo, Rui

doi:10.1016/j.msec.2017.02.102

Cited by 31 publications

(20 citation statements)

References 36 publications

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“…The bioresorbable alginate scaffold has been used for drug or cell delivery to provide spinal cord injury repair and axonal regeneration. 7 Currently a combinatorial approach integrating biomaterial scaffolds and biomolecule delivery seems to be more promising for regeneration and functional recovery. 7 In this study, we developed a sponge-like alginate scaffold that slowly releases human umbilical cord MSC (UCMSC) exosomes as a novel therapeutic approach for L5/6 spinal nerve ligation (SNL)-induced pain, a classical neuropathic pain model.…”

Section: Introductionmentioning

confidence: 99%

“…7 Currently a combinatorial approach integrating biomaterial scaffolds and biomolecule delivery seems to be more promising for regeneration and functional recovery. 7 In this study, we developed a sponge-like alginate scaffold that slowly releases human umbilical cord MSC (UCMSC) exosomes as a novel therapeutic approach for L5/6 spinal nerve ligation (SNL)-induced pain, a classical neuropathic pain model. 8 The primary aim of this study is to examine the effects of this exosome-scaffold (EX-SC), wrapped around ligated nerves, on SNL-induced pain, neuron/glial activation and neuro-inflammation.…”

Section: Introductionmentioning

confidence: 99%

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<p>Locally Applied Stem Cell Exosome-Scaffold Attenuates Nerve Injury-Induced Pain in Rats</p>

Hsu¹,

Shiue²,

Yang³

et al. 2020

JPR

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Nerve injury-induced pain is difficult to treat. In this study, we developed an alginate scaffold with human umbilical cord mesenchymal stem cell exosomes (EX-SC) to treat nerve injury-induced pain. Materials and Methods: The scaffold was prepared and characterized for its physical traits and biocompatibility. In vitro studies of PC12 and HEK293 cells were used to evaluate the neuroprotective and neurotrophic effects of exosomes. Right L5/6 spinal nerve ligation (SNL) was performed in Sprague-Dawley rats to induce mechanical allodynia and thermal hyperalgesia, evaluated by von Frey hair and radiant heat tests. The EX-SC was wrapped around ligated L5/6 spinal nerves for treatment. Western blotting and immunofluorescence staining were used to evaluate neuron/glial activation, cytokines and neurotrophic factor of affected dorsal root ganglion (DRG). Results: In cell culture assay, the exosomes induce neurite outgrowth of PC12 cells and protect PC12 and HEK293 cells against formaldehyde acid treatment. On post-ligation day 21, rats receiving EX-SC had significantly higher median (interquartile range) withdrawal threshold and latency [14.1 (13.7-15.5) g, 14.2 (13.7-15.3) s] than saline-SC-treated rats [2.1 (1.7-3.0) g, 2.0 (1.8-2.4) s, P=0.02 and 0.002]. The EX-SC also attenuated SNL-induced upregulation of c-Fos, GFAP, Iba1, TNF-α and IL-1β, while enhancing the level of IL-10 and GDNF, in the ipsilateral L5/6 DRG. After implantation for 21 days, the EX-SC enhanced the expression of myelin basic protein and IL-10 in injured L5/6 axons. Conclusion: We demonstrate the EX-SC possesses antinociceptive, anti-inflammation and pro-neurotrophic effects in the SNL pain model. It could be a promising therapeutic alternative for nerve injury-induced pain.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

<p>Locally Applied Stem Cell Exosome-Scaffold Attenuates Nerve Injury-Induced Pain in Rats</p>

Hsu¹,

Shiue²,

Yang³

et al. 2020

JPR

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“…SF has superior mechanical properties compared to other natural polymers (Chien-Yu et al, 2016). Jiao et al (2017) fabricated SF/Alg composite scaffolds loaded with NGF, which significantly improved the motor function of rats, via a freeze-drying method. Gan et al (2016) developed another nerve guide conduit (NGC) made of cellulose and soy protein isolate (SPI) through a chemical precipitation method that successfully bridged and repaired the nerve defect in 10 mm defect rat sciatic nerve injury models.…”

Section: Natural Polymer-based Conduitsmentioning

confidence: 99%

Polymeric Guide Conduits for Peripheral Nerve Tissue Engineering

Jiang

Qian

Fan

et al. 2020

Front. Bioeng. Biotechnol.

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Peripheral nerve injuries (PNIs) are usually caused by trauma, immune diseases, and genetic factors. Peripheral nerve injury (PNI) may lead to limb numbness, muscle atrophy, and loss of neurological function. Although an abundance of theories have been proposed, very few treatments can effectively lead to complete recovery of neurological function. Autologous nerve transplantation is currently the gold standard. Nevertheless, only 50% of all patients were successfully cured using this method. In addition, it causes inevitable damage to the donor site, and available donor sites in humans are very limited. Tissue engineering has become a research hotspot aimed at achieving a better therapeutic effect from peripheral nerve regeneration. Nerve guide conduits (NGCs) show great potential in the treatment of PNI. An increasing number of scaffold materials, including natural and synthetic polymers, have been applied to fabricate NGCs for peripheral nerve regeneration. This review focuses on recent nerve guide conduit (NGC) composite scaffold materials that are applied for nerve tissue engineering. Furthermore, the development tendency of NGCs and future areas of interest are comprehensively discussed.

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“…Spinal cord injury (SCI) is one of the main causes of disability associated with the inevitable formation of a glial scar in the posttraumatic period, which impedes the regenerative axonal growth through the site of the lesion [1][2][3]. An extensive research of potential therapeutic solutions has not provided promising results so far [4][5], though some of the recently proposed therapeutic protocols, such as epidural stimulation, seem to be more encouraging [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

A new model of spinal cord injury by cryoapplication: Morphodynamics of histological changes of the spinal cord lesion

Telegin

Minakov

Chernov

et al. 2020

Preprint

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Up to 500,000 people worldwide suffer from spinal cord injuries (SCI) annually, according to the WHO. Animal models are essential for searching novel methodological guidelines and therapeutic agents for SCI treatment. We developed an original model of posttraumatic spinal cord glial scar in rats using cryoapplication. The method is based on cryodestruction of spinal cord tissue with liquid nitrogen. Thirty six male SD linear rats of SPF category were included in this experimental study. A T13 unilateral hemilaminectomy was performed with an operating microscope, as it was extremely important not to penetrate the dura mater, and liquid nitrogen was applied into the bone defect for one minute. The animals were euthanized at various intervals ranging from 1 to 60 days after inducing cryogenic trauma, their Th12-L1 vertebrae were removed “en bloc” and the segment of the spinal cord exposed to the cryoapplicator was carefully separated for histological examination. The study results demonstrated that cryoapplication of liquid nitrogen, provoking a local temperature of approximately minus 20°C, produced a highly standardized transmural defect which extended throughout the dorsoventral arrangement of the spinal cord and had an “hour-glass” shape. During the entire study period (1-60 post-injury days), the glial scarring process and the spinal cord defect were located within the surgically approached vertebral space (Th13). Unlike other available experimental models of SCI (compression, contusion, chemical, etc.), the present option is characterized by a minimal invasiveness (the hemilaminectomy is less than 1 mm wide), high precision and consistency. Also, there was a low interanimal variability in histological lesions and dimensions of the produced defect. The original design of cryoapplicator used in the study played a major role in achieving these results. The original technique of high-precision cryoapplication for inducing consistent morphodynamic glial scarring could facilitate a better understanding of the self-recovery processes of injured spinal cord and would be helpful for proposing new platforms for the development of therapeutic strategies.

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A bridging SF/Alg composite scaffold loaded NGF for spinal cord injury repair

Cited by 31 publications

References 36 publications

<p>Locally Applied Stem Cell Exosome-Scaffold Attenuates Nerve Injury-Induced Pain in Rats</p>

<p>Locally Applied Stem Cell Exosome-Scaffold Attenuates Nerve Injury-Induced Pain in Rats</p>

Polymeric Guide Conduits for Peripheral Nerve Tissue Engineering

A new model of spinal cord injury by cryoapplication: Morphodynamics of histological changes of the spinal cord lesion

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