A Brassica napus mRNA encoding a protein homologous to phospholipid transfer proteins, is expressed specifically in the tapetum and developing microspores

Foster, Gary D.; Robinson, Susan W.; Blundell, Robert; Roberts, Michael R.; Hodge, Rachel; Draper, John; Scott, Rod

doi:10.1016/0168-9452(92)90133-7

Cited by 58 publications

(34 citation statements)

References 20 publications

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“…This pattern of expression correlates well with the timing of lipid accumulation in the tapetum of the B. napus cv. Westar (Polowick and Sawhney, 1990) and with the expression of a phospholipid transfer protein found in the B. napus tapetum and microspores (Foster et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of two Brassica napus genes related to oleosins which are highly expressed in the tapetum

Robert¹,

Gerster²,

Allard

et al. 1994

The Plant Journal

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SummaryTwo highly homologous Brassica napus flower cDNA clones, Sta 41-2 and Sta 41-9, were isolated and characterized. These clones were shown to correspond to genes expressed in the tapetum from the early uninucleate microspore stage to the dinucleate stage. The predicted Sta 41-2 and Sta 41-9 proteins possessed characteristics similar to oleosins such as a polar N-terminal domain, a large relatively conserved hydrophobic domain and a long C-terminal domain which consisted of four different groups of repeats. In addition, like oleosins, the Sta 41-2 and Sta 41-9 proteins have a basic pl, lack a signal peptide and are found in a tissue which accumulates Iipids in small lipid bodies.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of two Brassica napus genes related to oleosins which are highly expressed in the tapetum

Robert¹,

Gerster²,

Allard

et al. 1994

The Plant Journal

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“…The oleo-pollenin is considered the major protein component of the pollenkitt (Murphy & Ross, 1998;Murhpy, 2001). LTP genes are specifically expressed in the tapetum and developing microspores of Brassica napus, Nicotiana tabacum, Lilium henryi and Zea mays (Koltunow et al 1990;Foster et al, 1992;Crossley et al, 1995;Lauga et al, 2000). Therefore, the detection of nsLTPs in the A. latifolia pollenkitt is an expected result, because one of the many functions attributed to the pollenkitt is to protect the pollen protoplast from fungi and bacteria (Paccini & Hesse, 2005), and the localisation of nsLTPs in the pollenkitt confirms this hypothesis.…”

Section: The Role Of Nsltpsmentioning

confidence: 99%

An approach to the knowledge of pollen and allergen diversity through lipid transfer protein localisation in taxonomically distant pollen grains

Suárez‐Cervera

Vega-Maray

Castells

et al. 2008

Grana

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“…It has been demonstrated that LTPs represent the most abundant protein associated with the extracellular wax of sporopollenin (68,69). Sporopollenin is built from extracellular lipids originating from the tapetum (56,70), and LTP function has been suggested to be involved in the transfer of these lipids to the microspore (71). The deposition of the exine begins soon after the completion of meiosis II (56) at the time the rice plants become most susceptible to cold damage.…”

Section: Identification Of Cold-responsive Proteins In Rice Anthersmentioning

confidence: 99%

Low Temperature Treatment at the Young Microspore Stage Induces Protein Changes in Rice Anthers

Imin

Kerim

Weinman

et al. 2006

Molecular & Cellular Proteomics

111

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Male reproductive development in rice is very sensitive to various forms of environmental stresses including low temperature. A few days of cold treatment (<20°C) at the young microspore stage induce severe pollen sterility and thus large grain yield reductions. To investigate this phenomenon, anther proteins at the early stages of microspore development, with or without cold treatment at 12°C, were extracted, separated by two-dimensional gel electrophoresis, and compared. The cold-sensitive cultivar Doongara and the relatively cold-tolerant cultivar HSC55 were used. The abundance of 37 anther proteins was changed more than 2-fold after 1, 2, and 4 days of cold treatment in cv. Doongara. Among them, one protein was newly induced, 32 protein spots were up-regulated, and four protein spots were down-regulated. Of these 37 protein spots, we identified two anther-specific proteins (putative lipid transfer protein and Osg6B) and a calreticulin that were down-regulated and a cystine synthase, a ␤-6 subunit of the 20 S proteasome, an H protein of the glycine cleavage system, cytochrome c oxidase subunit VB, an osmotin protein homologue, a putative 6-phosphogluconolactonase, a putative adenylate kinase, a putative cysteine proteinase inhibitor, ribosomal protein S12E, a caffeoyl-CoA O-methyltransferase, and a monodehydroascorbate reductase that were up-regulated. Identification of these proteins is available upon request. Accumulation of these proteins did not vary greatly after cold treatment in panicles of cv. Doongara or in the anthers of the cv. Rice (Oryza sativa L.) evolved in tropical and subtropical areas, and as a result it has the characteristic of being vulnerable to cold weather. In particular, the combination of the low temperature sensitivity and a maximal sensitivity to these temperatures at precise stages of pollen microspore development (1) makes cool midseason temperatures the major environmental limitation (other than soil and water) for growing rice in temperate regions and at high elevations in the tropics (2). During the life cycle of rice, pollen microspore development at the booting stage is known to be the most susceptible to cool weather damage (3). Cold damage at this reproductive stage of rice causes limitation to the yields of temperate-grown rice around the world and can reduce yields to 40% (3, 4). The main cause of this damage is pollen sterility resulting from low temperature damage at the early stage of microspore development (1). The developmental stage most sensitive to the various forms of environmental stress, including cold damage, is just after meiosis. That is tetrad to early microspore phase, or the young microspore stage (5). In cold-treated rice plants, both cytological and histological abnormalities are greater in the anthers than in the pistil or other organs of the flower, and the cold damage can be rescued by artificial pollination with healthy pollen (5). In this process of cold damage, anthers become smaller, and cells in the tapetal layer in the anthers that surrounds a...

show abstract

A Brassica napus mRNA encoding a protein homologous to phospholipid transfer proteins, is expressed specifically in the tapetum and developing microspores

Cited by 58 publications

References 20 publications

Molecular characterization of two Brassica napus genes related to oleosins which are highly expressed in the tapetum

Molecular characterization of two Brassica napus genes related to oleosins which are highly expressed in the tapetum

An approach to the knowledge of pollen and allergen diversity through lipid transfer protein localisation in taxonomically distant pollen grains

Low Temperature Treatment at the Young Microspore Stage Induces Protein Changes in Rice Anthers

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