A Bowman–Birk-Type Trypsin-Chymotrypsin Inhibitor from Broad Beans

Ye, X.Y.; Ng, T.B.; Rao, Pingfan

doi:10.1006/bbrc.2001.5965

Cited by 111 publications

(93 citation statements)

References 36 publications

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“…From 100 g of small pinto beans, about 141 mg of trypsin inhibitor could be collected using the protocol mentioned above. The yield was higher than that obtained in purification of many other trypsin inhibitors, such as broad bean, white cloud bean and Faba bean trypsin inhibitors ($11, $29 and $54 mg/100 g beans, respectively) [22][23][24] .…”

Section: Discussionmentioning

confidence: 65%

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A thermostable trypsin inhibitor with antiproliferative activity from small pinto beans

Chan

Zhang

Sze

et al. 2013

Journal of Enzyme Inhibition and Medicinal Chemistry

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Small pinto bean is a cultivar of Phaseolus vulgaris. It produces a 16-kDa trypsin inhibitor that could be purified using anion exchange and size chromatography. Q-Sepharose, Mono Q and Superdex 75 columns were employed for the isolation process. Small pinto bean trypsin inhibitor demonstrated moderate pH stability (pH 2-10) and marked heat stability, with its trypsin inhibitory activity largely retained after exposure to 100 C for half an hour. The activity was abolished in the presence of dithiothreitol, in a dose-dependent manner, implying that disulfide bonds in small pinto bean trypsin inhibitor are crucial for the activity. The trypsin inhibitor showed a blocked N-terminus. The trypsin inhibitor only slightly inhibited the viability of breast cancer MCF7 and hepatoma HepG2 cells at 125 mM.

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Section: Discussionmentioning

confidence: 65%

“…It is known that some trypsin inhibitors have antifungal activity 23,24 whereas others do not [25][26][27][28][29][30][31] .…”

Section: Discussionmentioning

confidence: 99%

A thermostable trypsin inhibitor with antiproliferative activity from small pinto beans

Chan

Zhang

Sze

et al. 2013

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…Data presented here are the cells plasmolysed by incubation with 20% (w/v) sucrose solution for 10 min immediately before the observations. inhibitors possessing anti-fungal activity from broad beans and corn (Chen et al, 1999;Ye et al, 2001). However, it is unlikely that the anti-fungal activity of RlemMLP2 is caused by the protease inhibitor activity, since RlemMLP1 also possesses protease inhibitor activity but does not show antifungal activity.…”

Section: Discussionmentioning

confidence: 99%

Characterization of cDNAs Encoding Two Distinct Miraculin-like Proteins and Stress-related Modulation of the Corresponding mRNAs in Citrus jambhiri Lush

et al. 2006

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Two distinct full-length cDNAs from rough lemon that encoded miraculin-like proteins were isolated by random amplification of cDNA ends (RACEs), based on sequence information from subtractive PCR previously described, and designated as RlemMLP1 and RlemMLP2. The transcripts of both RlemMLP1 and RlemMLP2 were not detected in leaves, or stems but accumulated in fruits. Transcripts accumulated to higher levels in leaves after wounding, inoculation with conidia of Alternaria alternata, or treatment with methyl jasmonate vapors. Treatment with methyl salicylate antagonized the signaling pathway of wounding. Treatment with methyl salicylate at 2 h after wounding significantly reduced wounding-induced gene expression of both RlemMLP1 and RlemMLP2. Protein products of these genes were obtained by using a prokaryotic expression system, and had protease inhibitor activity. RlemMLP2, but not RlemMLP1, contained a thaumatin motif, and only RlemMLP2 showed anti-fungal activity against Alternaria citri. Cellular localization analysis with RlemMLP1 or RlemMLP2 fused to a green fluorescence protein gene following transient translation using a particle bombardment in onion cells indicated that both RlemMLP1 and RlemMLP2 were localized to the cytosol. These evidences revealed that rough lemon RlemMLPs are likely to have defensive function against pathogens at least when host cells are broken by their infections.

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“…presence of proteases inhibited Mycosphaerella, Fusarium, Botrytis, and Alternaria pathogenicity (151,161,162), which implies that these proteases may indirectly modulate virulence by modulation of ambient pH (25,26,89,118,119,123).…”

Section: Ambient Ph and Pathogenicity Factorsmentioning

confidence: 99%

Virulence Regulation of Phytopathogenic Fungi by pH

Alkan

Espeso

Prusky³

2013

Antioxidants & Redox Signaling

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Significance: Postharvest pathogens can start its attack process immediately after spores land on wounded tissue, whereas other pathogens can forcibly breach the unripe fruit cuticle and then remain quiescent for months until fruit ripens and then cause major losses. Recent Advances: Postharvest fungal pathogens activate their development by secreting organic acids or ammonia that acidify or alkalinize the host ambient surroundings. Critical Issues: These fungal pH modulations of host environment regulate an arsenal of enzymes to increase fungal pathogenicity. This arsenal includes genes and processes that compromise host defenses, contribute to intracellular signaling, produce cell wall-degrading enzymes, regulate specific transporters, induce redox protectant systems, and generate factors needed by the pathogen to effectively cope with the hostile environment found within the host. Further, evidence is accumulating that the secreted molecules (organic acids and ammonia) are multifunctional and together with effect of the ambient pH, they activate virulence factors and simultaneously hijack the plant defense response and induce program cell death to further enhance their necrotrophic attack. Future Directions: Global studies of the effect of secreted molecules on fruit pathogen interaction, will determine the importance of these molecules on quiescence release and the initiation of fungal colonization leading to fruit and vegetable losses.

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A Bowman–Birk-Type Trypsin-Chymotrypsin Inhibitor from Broad Beans

Cited by 111 publications

References 36 publications

A thermostable trypsin inhibitor with antiproliferative activity from small pinto beans

A thermostable trypsin inhibitor with antiproliferative activity from small pinto beans

Characterization of cDNAs Encoding Two Distinct Miraculin-like Proteins and Stress-related Modulation of the Corresponding mRNAs in Citrus jambhiri Lush

Virulence Regulation of Phytopathogenic Fungi by pH

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