A bouquet of chromosomes

Harper, Lisa C.; Golubovskaya, Inna N.; Cande, W. Zacheus

doi:10.1242/jcs.01363

Cited by 220 publications

(207 citation statements)

References 53 publications

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“…During early zygotene, telomere bouquets form in most organisms, making homologous chromosomes meet and pair more efficiently (Scherthan, 2001;Harper et al, 2004). The absence of typical zygotene chromosome organization in zygo1 triggers our suspicion about the impacts of ZYGO1 deficiency on telomere bouquet formation.…”

Section: Loss Of Zygo1 Causes Failure Of Telomere Bouquet Formationmentioning

confidence: 99%

“…Along with chromosomal movement, telomere bouquets form in many species; these structures are widely thought to efficiently facilitate homology searching and pairing by bringing distant chromosomes into a more confined area (Trelles-Sticken et al, 2000;Scherthan, 2001;Harper et al, 2004). Factors affecting telomere bouquet formation mainly participate in two aspects of telomere behavior: telomere attachment to the INM and subsequent telomere movement along the INM.…”

Section: Introductionmentioning

confidence: 99%

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The F-Box Protein ZYGO1 Mediates Bouquet Formation to Promote Homologous Pairing, Synapsis, and Recombination in Rice Meiosis

et al. 2017

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Telomere bouquet formation, a highly conserved meiotic event, plays an important role in homologous pairing and therefore progression of meiosis; however, the underlying molecular mechanism remains largely unknown. Here, we identified ZYGOTENE1 (ZYGO1), a novel F-box protein in rice (Oryza sativa), and verified its essential role in bouquet formation during early meiosis. In zygo1 mutants, zygotene chromosome aggregation and telomere clustering failed to occur. The suppressed telomere clustering in homologous pairing aberration in rice meiosis1 (pair1) zygo1 and rice completion of meiotic recombination (Oscom1) zygo1 double mutants, together with the altered localization of OsSAD1 (a SUN protein associated with the nuclear envelope) in zygo1, showed that ZYGO1 has a significant function in bouquet formation. In addition, the interaction between ZYGO1 and rice SKP1-like protein 1 suggested that ZYGO1 might modulate bouquet formation as a component of the SKP1-Cullin1-F-box complex. Although double-strand break formation and early recombination element installation occurred normally, zygo1 mutants showed defects in full-length pairing and synaptonemal complex assembly. Furthermore, crossover (CO) formation was disturbed, and foci of Human enhancer of invasion 10 were restricted to the partially synapsed chromosome regions, indicating that CO reduction might be caused by the failure of full-length chromosome alignment in zygo1. Therefore, we propose that ZYGO1 mediates bouquet formation to efficiently promote homolog pairing, synapsis, and CO formation in rice meiosis.

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Section: Loss Of Zygo1 Causes Failure Of Telomere Bouquet Formationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The F-Box Protein ZYGO1 Mediates Bouquet Formation to Promote Homologous Pairing, Synapsis, and Recombination in Rice Meiosis

et al. 2017

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“…Bouquet formation is a key event of a general repositioning of chromosomes and is thought to facilitate homologous chromosome pairing and synapsis (Harper et al, 2004;Zickler, 2006). To examine bouquet formation, we conducted fluorescence in situ hybridization (FISH) analysis using pAtT4 con- OsRec8 encodes a rice OsRad21-4 protein.…”

Section: Osrec8 Mutants Show Defective Telomere Bouquet Formationmentioning

confidence: 99%

OsREC8 Is Essential for Chromatid Cohesion and Metaphase I Monopolar Orientation in Rice Meiosis

Shao

Tang²,

Wang³

et al. 2011

Plant Physiol.

119

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The successful transmission of chromosomes during mitosis and meiosis relies on the establishment and subsequent release of cohesion between replicated chromatids. Cohesion is mediated by a four-subunit structural maintenance of chromosome complex, called cohesins. REC8 is a key component of this meiotic cohesion complex in most model organisms studied to date. Here, we isolated and dissected the functions of OsREC8, a rice (Oryza sativa) REC8 homolog, using two null Osrec8 mutants. We showed that OsREC8 encodes a protein that localized to meiotic chromosomes from approximately meiotic interphase to metaphase I. Homologous pairing and telomere bouquet formation were abnormal in Osrec8 meiocytes. Furthermore, fluorescent in situ hybridization experiments on Osrec8 meiocytes demonstrated that the mutation eliminated meiotic centromeric cohesion completely during prophase I and also led to the bipolar orientation of the kinetochores during the first meiotic division and accordingly resulted in premature separation of sister chromatid during meiosis I. Immunolocalization analyses revealed that the loading of PAIR2, PAIR3, OsMER3, and ZEP1 all depended on OsREC8. By contrast, the presence of the OsREC8 signal in pair2, pair3, Osmer3, and zep1 mutants indicated that the loading of OsREC8 did not rely on these four proteins. These results suggest that OsREC8 has several essential roles in the meiotic processes.

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“…In maize phs1 (poor homologous synapsis 1) meiocytes, loading of RAD51 complexes that catalyze the single-strand invasion step in recombination is greatly inhibited and results in synapsis among non-homologues [15]. Moreover, in many species, an interesting pre-synaptic configuration termed the chromosome bouquet, in which all telomeres are spatially attached to the nucleus envelope and clustered within a limited area, is thought to facilitate the homology search [16]. The pam1 (plural abnormalities of meiosis 1) mutant of maize, which is defective in telomere clustering, has normal RAD51 foci on zygotene chromosomes, but shows aberrant synapsis and a dramatic reduction in homologous pairing [17].…”

Section: Introductionmentioning

confidence: 99%

“…In Sordaria, homologs have completed homology search before the bouquet formation, and the maize phs1 mutation has little effect on bouquet formation in ~50% of the meiocytes. Moreover, Caenorhabditis elegans and Drosophila lack a bouquet altogether, but can undergo complete meiosis [16].…”

Section: Introductionmentioning

confidence: 99%

OsAM1 is required for leptotene-zygotene transition in rice

et al. 2011

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The events occurring at the onset of meiosis have not been fully elucidated. In the present study, OsAM1 was identified in rice (Oryza sativa L.) by map-based cloning. OsAM1, a homolog of Arabidopsis SWI1 and maize AM1, encodes a protein with a coiled-coil domain in its central region. In the Osam1 mutant, pollen mother cells are arrested at leptotene, showing that OsAM1 is required for the leptotene-zygotene transition. Immunocytological analysis revealed that OsAM1 exists as foci in early prophase I meiocytes. Very faint OsREC8 foci persisted in the Osam1 mutant, indicating that OsAM1 is not required for the initial meiotic recruitment of OsREC8. In the absence of OsAM1, many other critical meiotic components, including PAIR2, ZEP1 and OsMER3, could not be correctly installed onto chromosomes. In contrast, in pair2, Osmer3 and zep1 mutants, OsAM1 could be loaded normally, suggesting that OsAM1 plays a fundamental role in building the proper chromosome structure at the beginning of meiosis.

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A bouquet of chromosomes

Cited by 220 publications

References 53 publications

The F-Box Protein ZYGO1 Mediates Bouquet Formation to Promote Homologous Pairing, Synapsis, and Recombination in Rice Meiosis

The F-Box Protein ZYGO1 Mediates Bouquet Formation to Promote Homologous Pairing, Synapsis, and Recombination in Rice Meiosis

OsREC8 Is Essential for Chromatid Cohesion and Metaphase I Monopolar Orientation in Rice Meiosis

OsAM1 is required for leptotene-zygotene transition in rice

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