A boost for fish skeletal research

Witten, P. Eckhard; Huysseune, Ann; Maisey, John G.; Winkler, Christoph; Gong, Zhiyuan

doi:10.1111/jfb.14556

Cited by 3 publications

(3 citation statements)

References 36 publications

(38 reference statements)

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“…(75) Because of evolutionarily conserved genetic, developmental, and compositional similarities between bones of teleosts and mammals, not only zebrafish (19,(76)(77)(78)(79) but also medaka are used to model skeletal diseases and test drug targets. (11,19,(76)(77)(78)(79)(80)(81) The diversity of laboratory teleost species offers additional advantages in the scope of both intervention studies and studies of aging-related bone degeneration. Killifish with a life span of up to 6 months, for example, might reduce timeintensive experiments in longer-lived species.…”

Section: In Vivo Models For Skeletal Research: State Of the Artmentioning

confidence: 99%

Why Animal Experiments Are Still Indispensable in Bone Research: A Statement by the European Calcified Tissue Society

Stein,

Elefteriou,

Busse

et al. 2023

J of Bone & Mineral Res

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Major achievements in bone research have always relied on animal models and in vitro systems derived from patient and animal material. However, the use of animals in research has drawn intense ethical debate and the complete abolition of animal experimentation is demanded by fractions of the population. This phenomenon is enhanced by the reproducibility crisis in science and the advance of in vitro and in silico techniques. 3D culture, organ‐on‐a‐chip, and computer models have improved enormously over the last few years. Nevertheless, the overall complexity of bone tissue cross‐talk and the systemic and local regulation of bone physiology can often only be addressed in entire vertebrates. Powerful genetic methods such as conditional mutagenesis, lineage tracing, and modeling of the diseases enhanced the understanding of the entire skeletal system. In this review endorsed by the European Calcified Tissue Society (ECTS), a working group of investigators from Europe and the US provides an overview of the strengths and limitations of experimental animal models, including rodents, fish, and large animals, as well the potential and shortcomings of in vitro and in silico technologies in skeletal research. We propose that the proper combination of the right animal model for a specific hypothesis and state‐of‐the‐art in vitro and/or in silico technology is essential to solving remaining important questions in bone research. This is crucial for executing most efficiently the 3R principles to reduce, refine, and replace animal experimentation, for enhancing our knowledge of skeletal biology, and for the treatment of bone diseases that affect a large part of society. © 2023 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).

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Section: In Vivo Models For Skeletal Research: State Of the Artmentioning

confidence: 99%

Why Animal Experiments Are Still Indispensable in Bone Research: A Statement by the European Calcified Tissue Society

Stein,

Elefteriou,

Busse

et al. 2023

J of Bone & Mineral Res

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“…Also, an important work by Witten et al reminds us about the commonalities and specifics of small teleost biology in response to their environments [ 166 ]. Zebrafish and medaka belong to two different orders within the ray-finned fishes and have a long evolutionary divergence time [ 166 ]. The teleost skeleton is a prominent place for lipid storage [ 166 ].…”

Section: Zebrafish As Animal Models To Accelerate Discoveries Of Huma...mentioning

confidence: 99%

“…Zebrafish and medaka belong to two different orders within the ray-finned fishes and have a long evolutionary divergence time [ 166 ]. The teleost skeleton is a prominent place for lipid storage [ 166 ]. A relatively limiting factor is the fact that teleost and other primary aquatic gnathostomes (vertebrates with jaws) are able to effectively obtain calcium from the water via the gills but they depend on dietary phosphorous intake for the mineralization of the skeleton [ 166 ].…”

Section: Zebrafish As Animal Models To Accelerate Discoveries Of Huma...mentioning

confidence: 99%

Functional Validation of Osteoporosis Genetic Findings Using Small Fish Models

Kague

Karasik

2022

Genes

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The advancement of human genomics has revolutionized our understanding of the genetic architecture of many skeletal diseases, including osteoporosis. However, interpreting results from human association studies remains a challenge, since index variants often reside in non-coding regions of the genome and do not possess an obvious regulatory function. To bridge the gap between genetic association and causality, a systematic functional investigation is necessary, such as the one offered by animal models. These models enable us to identify causal mechanisms, clarify the underlying biology, and apply interventions. Over the past several decades, small teleost fishes, mostly zebrafish and medaka, have emerged as powerful systems for modeling the genetics of human diseases. Due to their amenability to genetic intervention and the highly conserved genetic and physiological features, fish have become indispensable for skeletal genomic studies. The goal of this review is to summarize the evidence supporting the utility of Zebrafish (Danio rerio) for accelerating our understanding of human skeletal genomics and outlining the remaining gaps in knowledge. We provide an overview of zebrafish skeletal morphophysiology and gene homology, shedding light on the advantages of human skeletal genomic exploration and validation. Knowledge of the biology underlying osteoporosis through animal models will lead to the translation into new, better and more effective therapeutic approaches.

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Endless bones most beautiful: Book review of Vivian de Buffrénil, Armand J. de Ricqlès, Louise Zylberberg, Kevin Padian (eds) 2021 Vertebrate Skeletal Histology and Paleohistology

Sánchez-Villagra

2022

Swiss J Palaeontol

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A boost for fish skeletal research

Cited by 3 publications

References 36 publications

Why Animal Experiments Are Still Indispensable in Bone Research: A Statement by the European Calcified Tissue Society

Why Animal Experiments Are Still Indispensable in Bone Research: A Statement by the European Calcified Tissue Society

Functional Validation of Osteoporosis Genetic Findings Using Small Fish Models

Endless bones most beautiful: Book review of Vivian de Buffrénil, Armand J. de Ricqlès, Louise Zylberberg, Kevin Padian (eds) 2021 Vertebrate Skeletal Histology and Paleohistology

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