A biotechnological perspective on the application of iron oxide magnetic colloids modified with polysaccharides

Dias, Ana; Hussain, Abid; Marcos, A.S.; Roque, Ana C. A.

doi:10.1016/j.biotechadv.2010.10.003

Cited by 305 publications

(154 citation statements)

References 138 publications

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“…More detailed description of the physical phenomena subjacent to bio-applications of iron oxide nanoparticles can be found elsewhere (Daniel-da-Silva et al, 2011;Laurent et al, 2008). The biomedical and biotechnological applications of magnetic nanoparticles and their composites include magnetic separation, medical imaging, drug delivery and cancer hyperthermia (Dias et al, 2011;Laurent et al, 2008;Tartaj, 2011) and will be described in section 5 of this chapter. The properties required for the magnetic nanoparticles differ according to the application envisaged and are strongly dependent on the particle size and shape.…”

Section: Magnetic Nanoparticlesmentioning

confidence: 99%

“…In the last years, a variety of biopolymers have been investigated as soft matrices to accommodate inorganic nanoparticles (Darder et al, 2007;Dias et al, 2011). Innovation in Nanomedicine has been a major driving force to create new bionanocomposites because these materials bring together the intrinsic functionalities of inorganic nanoparticles and the biointerfaces offered by polymers of natural origin.…”

Section: Introductionmentioning

confidence: 99%

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Biofunctional Composites of Polysaccharides Containing Inorganic Nanoparticles

Trindade¹,

Daniel‐da‐Silva²

2011

Advances in Nanocomposite Technology

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Section: Magnetic Nanoparticlesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biofunctional Composites of Polysaccharides Containing Inorganic Nanoparticles

Trindade¹,

Daniel‐da‐Silva²

2011

Advances in Nanocomposite Technology

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“…Magnetic nanoparticles like iron oxides are of great interest for researchers from a wide range of disciplines, including magnetic fluids, catalysis, biotechnology/biomedicine, magnetic resonance imaging, data storage, and environmental remediation [1][2][3][4] . While a number of suitable methods have been developed for the synthesis of magnetic nanoparticles of various different compositions, successful application of such magnetic nanoparticles in the areas listed above is highly dependent on the stability of the particles under a range of different conditions.…”

Section: Introductionmentioning

confidence: 99%

Study of Thermal Degradation of PLGA, PLGA Nanospheres and PLGA/Maghemite Superparamagnetic Nanospheres

et al. 2015

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Poly(glycolide-co-lactide) (PLGA) nanospheres containing magnetic materials have been extensively studied because of its biomedical applications. Therefore, it is very important to know thermal properties of these materials in addition to other physical properties. Thermal degradation activation energy (E α ) of PLGA nanospheres with maghemite entrapment (PLGA-Mag), PLGA nanospheres (hollow spheres) (PLGA-H) obtained by an emulsion method and unprocessed PLGA (PLGA-R) were calculated by isoconversional Vyazovkin method based on data of TG analysis in order to evaluate modifications in thermal behavior caused by nanospheres obtainment process or by maghemite entrapment. Both hydrodynamic diameter in the range of 200-250 nm and polydispersity index lower than 0.3 are considered satisfactory. Thermal degradation of PLGA-R begins at higher temperatures than those of PLGA-H and PLGA-Mag, but processed samples presented increase in thermal stability, which was greater before processing by emulsion and in the presence of the magnetic materials. PLGA-Mag presents superparamagnetic behavior at room temperature.

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“…Further, they confer chemical functionality to nanostructures such as Fe 3 O 4 MNPs. 26 In a previous study, 27 we synthesized and characterized Fe 3 O 4 MNPs using a brown seaweed (Sargassum muticum) extract via the green method. The aim of this study was to investigate the cytotoxic effects of Fe 3 O 4 MNPs prepared by green biosynthesis on various human cancer cell lines using a number of experimental methods.…”

mentioning

confidence: 99%

Cytotoxic effect of magnetic iron oxide nanoparticles synthesized via seaweed aqueous extract

Namvar

Rahman

Mohamad

et al. 2014

IJN

224

109

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Magnetic iron oxide nanoparticles (Fe 3 O 4 MNPs) are among the most useful metal nanoparticles for multiple applications across a broad spectrum in the biomedical field, including the diagnosis and treatment of cancer. In previous work, we synthesized and characterized Fe 3 O 4 MNPs using a simple, rapid, safe, efficient, one-step green method involving reduction of ferric chloride solution using brown seaweed ( Sargassum muticum ) aqueous extract containing hydroxyl, carboxyl, and amino functional groups mainly relevant to polysaccharides, which acts as a potential stabilizer and metal reductant agent. The aim of this study was to evaluate the in vitro cytotoxic activity and cellular effects of these Fe 3 O 4 MNPs. Their in vitro anticancer activity was demonstrated in human cell lines for leukemia (Jurkat cells), breast cancer (MCF-7 cells), cervical cancer (HeLa cells), and liver cancer (HepG2 cells). The cancer cells were treated with different concentrations of Fe 3 O 4 MNPs, and an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was used to test for cytotoxicity, resulting in an inhibitory concentration 50 (IC 50 ) value of 23.83±1.1 μg/mL (HepG2), 18.75±2.1 μg/mL (MCF-7), 12.5±1.7 μg/mL (HeLa), and 6.4±2.3 μg/mL (Jurkat) 72 hours after treatment. Therefore, Jurkat cells were selected for further investigation. The representative dot plots from flow cytometric analysis of apoptosis showed that the percentages of cells in early apoptosis and late apoptosis were increased. Cell cycle analysis showed a significant increase in accumulation of Fe 3 O 4 MNP-treated cells at sub-G1 phase, confirming induction of apoptosis by Fe 3 O 4 MNPs. The Fe 3 O 4 MNPs also activated caspase-3 and caspase-9 in a time-response fashion. The nature of the biosynthesis and therapeutic potential of Fe 3 O 4 MNPs could pave the way for further research on the green synthesis of therapeutic agents, particularly in nanomedicine, to assist in the treatment of cancer.

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A biotechnological perspective on the application of iron oxide magnetic colloids modified with polysaccharides

Cited by 305 publications

References 138 publications

Biofunctional Composites of Polysaccharides Containing Inorganic Nanoparticles

Biofunctional Composites of Polysaccharides Containing Inorganic Nanoparticles

Study of Thermal Degradation of PLGA, PLGA Nanospheres and PLGA/Maghemite Superparamagnetic Nanospheres

Cytotoxic effect of magnetic iron oxide nanoparticles synthesized via seaweed aqueous extract

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