A Biostimulant Seed Treatment Improved Heat Stress Tolerance During Cucumber Seed Germination by Acting on the Antioxidant System and Glyoxylate Cycle

Campobenedetto, Cristina; Grange, Eric; Mannino, Giuseppe; Arkel, Jeroen van; Beekwilder, Jules; Karlova, Rumyana; Garabello, Christian; Contartese, Valeria; Bertea, Cinzia M.

doi:10.3389/fpls.2020.00836

Cited by 51 publications

(38 citation statements)

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“…Seed treatment technologies consist of seed treatment with synthetic or natural compounds, which are aimed at enhancing the uniformity and vigor of seedlings and improving the plant tolerance to abiotic stresses [ 28 ]. The use of biostimulants to counteract the effect of abiotic stress is well-recognized and their function in promoting plant defenses against adverse environmental conditions has been studying lately [ 9 , 12 ]. Seed pretreatments have been revealed as a good method to contrast the ever-increasing environmental stress presence, improving the yield of productions, starting from seed germination [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…During these field trials, the biostimulant applied as seed treatment on soybean, was able to positively affect plant vegetative and reproductive growth under natural heat stress conditions. The biostimulant seed treatment efficiency for improving germination and mitigating oxidative damages under heat stress conditions has been observed in our previous work on cucumber seeds [ 9 ]. In the present work, in order to evaluate the potential effects of the biostimulant in promoting heat stress tolerance also in soybean, we conducted germination trials under controlled conditions, and we investigated the potential mechanism of action using combined biochemical and transcriptomic (RNA-Seq) approaches.…”

Section: Discussionmentioning

confidence: 99%

“…In this way, once the seeds are sown under normal or stress conditions, they exhibit a faster and more homogeneous germination, together with a higher tolerance to stresses. Similar to priming treatments, seed treatments make the seed more responsive to external stimuli and able to counteract stress conditions before seedling emergence [ 7 , 9 ]. So far, in the literature there are few references about biostimulants used for these purposes [ 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…In this context, seed treatments using biostimulants have been proposed as agronomic tools to counteract the negative effects of abiotic stresses. Although the action mechanisms of these products are not well known, scientific evidences suggest that the beneficial properties are linked to their content in bioactive molecules, which are able to improve a plant’s capability to face adverse environmental conditions, affecting the primary or secondary metabolism [ 9 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…In this study, we evaluated the efficiency of a seed treatment with a biostimulant based on lignin derivatives (lignosulphonates) that also contain plant-derived amino acids and molybdenum [ 9 ], for promoting soybean tolerance to heat stress. This product was especially studied for the Brazilian market, since, in this country, adverse conditions at sowing time are often present.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analyses and Antioxidant Activity Profiling Reveal the Role of a Lignin-Derived Biostimulant Seed Treatment in Enhancing Heat Stress Tolerance in Soybean

Campobenedetto

Mannino

Agliassa

et al. 2020

Plants

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Soybean (Glycine max Merr.) is a worldwide important legume crop, whose growth and yield are negatively affected by heat stress at germination time. Here, we tested the role of a biostimulant based on lignin derivatives, plant-derived amino acids, and molybdenum in enhancing soybean heat stress tolerance when applied on seeds. After treatment with the biostimulant at 35 °C, the seed biometric parameters were positively influenced after 24 h, meanwhile, germination percentage was increased after 72 h (+10%). RNA-Seq analyses revealed a modulation of 879 genes (51 upregulated and 828 downregulated) in biostimulant-treated seeds as compared with the control, at 24 h after incubation at 35 °C. Surprisingly, more than 33% of upregulated genes encoded for ribosomal RNA (rRNA) methyltransferases and proteins involved in the ribosome assembly, acting in a specific protein network. Conversely, the downregulated genes were involved in stress response, hormone signaling, and primary metabolism. Finally, from a biochemical point of view, the dramatic H2O2 reduction 40%) correlated to a strong increase in non-protein thiols (+150%), suggested a lower oxidative stress level in biostimulant-treated seeds, at 24 h after incubation at 35 °C. Our results provide insights on the biostimulant mechanism of action and on its application for seed treatments to improve heat stress tolerance during germination.

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