A biosensor for the determination of high density lipoprotein cholesterol employing combined surfactant-derived selectivity and sensitivity enhancements

Abstract

A biosensor for the determination of high density lipoprotein cholesterol employing combined surfactant-derived selectivity and sensitivity enhancements. Analytical Methods, 6. pp. 3975-3981. ISSN 1759-9660 Available from: http://eprints.uwe.ac.uk/23377We recommend you cite the published version. The publisher's URL is: http://dx.doi.org/10.1039/C3AY42262C Refereed: YesPublished online first 7 Mar 2014 Disclaimer UWE has obtained warranties from all depositors as to their title in the material deposited and as to their right to deposit such material. UWE makes no representation or warranties of commercial utility, title, or fitness for a particular purpose or any other warranty, express or implied in respect of any material deposited. UWE makes no representation that the use of the materials will not infringe any patent, copyright, trademark or other property or proprietary rights. UWE accepts no liability for any infringement of intellectual property rights in any material deposited but will remove such material from public view pending investigation in the event of an allegation of any such infringement. PLEASE SCROLL DOWN FOR TEXT.Journal Name High density lipoprotein cholesterol (HDL-C) is a modifiable risk factor in cardiovascular disease and devices suitable for its determination at the point of care are critical to the future management of hypercholesterolaemia. An electrochemical biosensor for measuring HDL-C was developed. The 10 biosensor was based on a homogeneous assay methodology for selective determination of HDL-C in combination with a printed electrochemical sensor for measuring the reduction of hydrogen peroxide at a silver paste electrode. The polyoxyethylene alkylene tribenzylphenyl ether surfactant (Emulgen B-66) was found to be capable of both the selective dissolution of HDL particles, as well as the enhanced electrocatalytic reduction of hydrogen peroxide. The resulting biosensor was shown to have a linear 15 response to HDL-C from 0.5 to 4 mM (r 2 =0.998) with an average r.s.d. of 7%. The biosensor was also used to analyse HDL-C in thirteen serum samples and had good agreement with a commercial spectrophotometric precipitation-based assay (r=0.7222; p < 0.058). IntroductionCholesterol levels, including high density lipoprotein cholesterol 20 (HDL-C) are modifiable risk factors for cardiovascular disease (CVD), a condition which remains the number one global cause of death. 1-3 Levels of HDL-C above 60 mg/dL (1.55 mM) are considered to have a positive protective role in heart disease, while low HDL-C levels (less than 40 mg/dL or about 1 mM) are 25 linked to an increase in heart attack risk. For this reason, the importance of measurement of HDL-C has been emphasized by the National Cholesterol Education Programme (NCEP) since the late 1980s. 4,5 The measurement of HDL-C is also important for two other purposes: Network (CRMLN) developed a simpler method based on a modified dextran sulphate procedure. 8 However, this technique, like the CDC method, also required large sample volumes and a...

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