A Bioremediation Approach to Mercury Removal in a Shake Flask Culture Using Pseudomonas putida (ATCC 49128)

Azoddein, Abd Aziz Bin Mohd; Ahmad, Mani Malam

doi:10.4172/2155-9872.1000312

Cited by 3 publications

(2 citation statements)

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“…An appreciable sulfide utilization indicated by its oxidation was recorded within the first six hours of inoculation, with 130 ppm/hr (65%), 220 ppm/hr (73.33) and 390 ppm/hr (78%) in 200 ppm, 300 ppm and 500 ppm, respectively. This was also compensated by an exponential cell biomass growth range of 2.62 to 3.91 and cell biomass increase measured by cell dry weight of 0.02 cell g/L to 0.59 cell g/L (Table 1-3).. Bacterial cell growth within the initial 6-8 hours of inoculation was shown to be the fastest phase due to efficient exchange of electrons between donors and acceptors as well as available nutrient source for rapid cell division and absence of accumulated metabolites residue that used to slowed cell growth (Azoddein, et al, 2016;Azoddein, et al, 2015). However, this sudden growth was followed by a decrease in cell growth probably due to inhibitory effects of free soluble form and undissociated H 2 S which permeate cell membranes and form cross-links between polypeptide chains, thus altering cell proteins, coenzyme activities (Lorna Guerreroa et al, 2015).…”

Section: Results and Discussion Effect Of Sulfide Concentration Levelmentioning

confidence: 96%

Biological Sulfide Oxidation and Its Impact on Cell Biomass Synthesis by Mesophilic Bacterium Bacillus Cerues (Atcc 14579)

Ahmad¹,

Aziz²,

Khushairi³

et al. 2017

JCEIB

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Inhibitory effects of hydrogen sulfide could either be due to toxicity to living tissue or precipitation of sulfide salts which inhibit its utilization for cell growth. Growth, is undoubtedly affected by certain conditions, among which are nutrient types and availability, as well as the operational parameters of the fermenting medium. In this study, Bacillus cereus (ATCC 14579) was tested for potential sulfide biodegradation in a suspended growth medium of orbital shake flasks using a single milieu composition under defined operational parameters. Growth and sulfide oxidation efficiency were measured spectrophotometrically under optimum physical conditions of pH, temperature, acclimatization time and agitation. Sulfide reduction was overwhelmingly recorded at three different sulfide loading rates of 200 ppm S2- L-1 d- 1, 300 ppm S2- L-1 d-1 and 500 ppm S2- L-1 d-1 with corresponding appreciable cell growth measured at OD600 nm. Results indicated that it was possible to realise sulfide removal efficiency of 95% to 99% using this strain in an orbital shake flask within 24 hrs, as well as 65% to 78% within the first 6 hrs of inoculation. Overall, sulfide wasreduced by 95% in 200 ppm and 300 ppm, while 99% in 500 ppm, respectively. While, the corresponding exponential cell growth recorded was 3.91, 3.80 and 3.61 in 200 ppm, 300 ppm and 500 ppm, respectively. This also translate to cell biomass synthesis (cell dry weight) of 0.61 g/L, 0.58 g/L and 0.50 g/L in 200 ppm, 500 ppm and 300 ppm. Based on this finding, it was clear that this inoculum can utilize different sulfide concentration for growth and biosynthesis; thus can be employed to treat sulfide contaminated wastewater in a suspended growth form under simple nutrient composition and operational conditions.

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Section: Results and Discussion Effect Of Sulfide Concentration Levelmentioning

confidence: 96%

Biological Sulfide Oxidation and Its Impact on Cell Biomass Synthesis by Mesophilic Bacterium Bacillus Cerues (Atcc 14579)

Ahmad¹,

Aziz²,

Khushairi³

et al. 2017

JCEIB

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“…This assertion could be deduced from the sulfide reduction rate in relation to cell biomass accumulation (CDW), which is given as the ratio of concentration sulfide to cell biomass (ppm H 2 S/g cell). This inverse relationship indicated an increase in CDW as sulfide concentration is further depleted, probably signaling sulfide uptake, and assimilation by the growing and dividing [22,23].…”

Section: Resultsmentioning

confidence: 99%

Eco-friendly Industrial wastewater treatment: Potential of mesophilic bacterium, Pseudomonas putida (ATCC 49128) for hydrogen sulfide oxidation

2018

J App Biol Biotech

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The effectiveness and environmental friendliness of biological sulfide oxidation have endeared it to many researchers, mainly for its potential to offer the best alternative for the evacuation of various forms of sulfide. The present study was conducted to assess the potential of gammaproteobacteria, Pseudomonas putida (ATCC 49128), to biodegrade sulfide significantly in a suspended medium of batch reactor type. Sulfide oxidation efficiency was measured spectrophotometrically under defined operational conditions of temperature, agitation, aeration, and influent sulfide concentrations. Sulfide reduction rates were observed at three disproportionate sulfide concentrations of 100 ppm S, and 500 ppm S. The simple statistical analysis was employed in the interpretation of the data and presented graphically. The results indicated that it was possible to realize sulfide removal efficiency of 45-70% within the first 6 h of start-up and 96-100% in 24 h period. On the other hand, the corresponding exponential cell growth recorded was 3.91, 3.80, and 3.61 in 100 ppm, 300 ppm, and 500 ppm, respectively. This also translates to cell biomass synthesis (cell dry weight) of 0.61 g/L, 0.58 g/L, and 0.50 g/L in 100, 500, and 300 ppm, respectively. In conclusion, it can be deduced that this inoculum can utilize different sulfide concentration for its growth and biosynthesis and thus can be employed to treat sulfide contaminated wastewater in a suspended growth form.

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Characterization of mercury-reducing potential bacteria isolated from Keputih non-active sanitary landfill leachate, Surabaya, Indonesia under different saline conditions

Imron

Kurniawan²,

Soegianto

2019

Journal of Environmental Management

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A Bioremediation Approach to Mercury Removal in a Shake Flask Culture Using Pseudomonas putida (ATCC 49128)

Cited by 3 publications

References 0 publications

Biological Sulfide Oxidation and Its Impact on Cell Biomass Synthesis by Mesophilic Bacterium Bacillus Cerues (Atcc 14579)

Biological Sulfide Oxidation and Its Impact on Cell Biomass Synthesis by Mesophilic Bacterium Bacillus Cerues (Atcc 14579)

Eco-friendly Industrial wastewater treatment: Potential of mesophilic bacterium, Pseudomonas putida (ATCC 49128) for hydrogen sulfide oxidation

Characterization of mercury-reducing potential bacteria isolated from Keputih non-active sanitary landfill leachate, Surabaya, Indonesia under different saline conditions

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