A biophysical study of the interactions between the antimicrobial peptide indolicidin and lipid model systems

Lipid membrane interactions of self-assembling antimicrobial nanofibers: effect of PEGylation

“…The thickness and area per lipid calculated for the pristine bilayers (Table 1) are comparable with literature values based on MD simulations on DMPC/DMPG phospholipids [46][47][48] and previous NR results. 31 Addition of the shorter 3W32 peptide had only a slight effect on the membrane structure (Fig. 2).…”

“…S3 †) and probably they insert as single chains in the membrane similar to other AMPs having a random coil structure such as indolicidin. 31 However, when comparing to indolicidin, not only is the amount of inserted 3W32 in the hydrophobic lipid region signicantly lower, 31 but 3W32 seems unable to penetrate into the inner head group of the bilayer at this concentration. This might suggest that the amount of hydrophobic leucine groups is too low to provide sufficient driving force for membrane penetration.…”

“…[31][32][33][34][35][36][37][38] In an earlier work, we showed that NR results can be directly compared to results from detailed modelling of small angle X-ray scattering (SAXS) data on monomeric peptide lipid bilayer using SLBs or unilamellar vesicles respectively. 31 For supramolecular nanobers in particular, NR has an advantage over bulk methods since it lacks 3D orientation averaging and enables precise structural determination of complex MDP-membrane structures. Here, MDPs made of K 3 W(QL) 6 K 2 with and without PEGylation are used in combination with SLB constituted of DMPC/DMPG and studied by contrast variation NR.…”

Structure-Activity Study of an All-d Antimicrobial Octapeptide D2D

“…These results indicate that there is no evidence for membrane deformation or any particular indication of pore formation. However peptide membrane insertion has been suggested to cause lipid disordering resulting increased leakage of cell fluids [30].…”

“…The development of carbapenem resistance in these species has led to the re-introduction of the last resort antibiotic Colistin, which was initially abandoned due to side-effects. Since 2000, approximately 30 new antibiotics have been marketed worldwide of which five were first-in-class antibiotics [4]. However, these five new antibiotic classes linezolid, daptomycin, retapamulin, fidaxomicin and bedaquiline only target Gram-positive bacteria so there is an unmet need for new antibiotics targeting Gram-negative bacteria [4].…”