A Biophysical Analysis to Assess X-Ray Sensitivity of Healthy and Tumour Cells

Panzetta, Valeria; Pugliese, M.; Musella, Ida; Menna, Marta De; Netti, Paolo A.; Fusco, Sabato

doi:10.1093/rpd/ncy303

Cited by 4 publications

(10 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More recently, Hohmann et al observed that irradiation leads to changes in motility and to a less invasive phenotype of two glioblastoma cell lines, both associated with an increase of cell mechanical properties and changes in the cytoskeleton structure [12]. Our group reported similar results on healthy and tumor fibroblasts irradiated with 250 keV and 6 MeV [13][14][15]. A sensitive increase in the mechanical properties of tumor cells, responsible for the enhancement of cell-adhesion and the reduction of migration of tumor cells, was observed.…”

Section: Introductionsupporting

confidence: 76%

“…Selected doses were 2 and 10 Gy, which represent the daily dose in radiotherapy treatment and the single maximum dose for the treatment of metastasis. Time points of 1 and 3 days (d) after irradiation were chosen based on our previous observations according to which, at these particular time points, healthy and tumor cells exhibited peculiar and different responses to irradiation [13][14][15]. Shedding light on the effects of X-rays on functions strictly associated with the cytoskeleton architecture and to cell-ECM crosstalk in healthy and tumor cells can open new frontiers in the diagnostic and therapeutic use of RT.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Adhesion and Migration Response to Radiation Therapy of Mammary Epithelial and Adenocarcinoma Cells Interacting with Different Stiffness Substrates

Panzetta

Verde

Pugliese

et al. 2020

Cancers

View full text Add to dashboard Cite

The structural and mechanical properties of the microenvironmental context have a profound impact on cancer cell motility, tumor invasion, and metastasis formation. In fact, cells react to their mechanical environment modulating their adhesion, cytoskeleton organization, changes of shape, and, consequently, the dynamics of their motility. In order to elucidate the role of extracellular matrix stiffness as a driving force in cancer cell motility/invasion and the effects of ionizing radiations on these processes, we evaluated adhesion and migration as biophysical properties of two different mammary cell lines, over a range of pathophysiological stiffness (1–13 kPa) in a control condition and after the exposure to two different X-ray doses (2 and 10 Gy, photon beams). We concluded that the microenvironment mimicking the normal mechanics of healthy tissue has a radioprotective role on both cell lines, preventing cell motility and invasion. Supraphysiological extracellular matrix stiffness promoted tumor cell motility instead, but also had a normalizing effect on the response to radiation of tumor cells, lowering their migratory capability. This work lays the foundation for exploiting the extracellular matrix-mediated mechanism underlying the response of healthy and tumor cells to radiation treatments and opens new frontiers in the diagnostic and therapeutic use of radiotherapy.

show abstract

Section: Introductionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Adhesion and Migration Response to Radiation Therapy of Mammary Epithelial and Adenocarcinoma Cells Interacting with Different Stiffness Substrates

Panzetta

Verde

Pugliese

et al. 2020

Cancers

View full text Add to dashboard Cite

show abstract

“…Considering that cell motility is a very sophisticated process based on a repeated cycle of membrane protrusion, attachment to the ECM, CSK contraction, and rear detachment from the ECM, tightly controlled by the FA life cycle (assembly–maturation–disassembly), the authors evaluated the impact of radiation on CSK and FAs’ components. In particular, they observed an increase in the organization and polymerization of actin filaments and to a consequent stiffening of the CSK, as supported by particle tracking microrheology (PTM) and atomic force microscopy (AFM) experiments [ 69 , 70 , 116 , 117 ]. As expected, the CSK stiffening was associated with the formation of longer FAs, which reduce cell migration speed because of their greater lifetime [ 131 ].…”

Section: Radiation Effects On Cell Migrationmentioning

confidence: 91%

“…For instance, the study performed on two different fibroblast cell lines, BALBc/3T3 and SVT2, showed that ionizing radiations altered the CSK structure, which, in turn, modified cells’ adhesive abilities. It was found that in control condition the cancerous cell line exhibited a disorganized actin CSK and a reduced adhesion in comparison with normal cells, after irradiation cell adhesiveness increased for both cell lines as evidenced by the enhancement of both spreading areas, FAs size, and cell stiffness [ 69 , 116 , 117 , 118 ]. Another study has investigated the alterations in the adhesion abilities of two mammary cell lines, MCF10A and the tumours counterpart MDA-MB-231, before and after the delivery of two doses of X-rays (2 Gy and 10 Gy) [ 119 ].…”

Section: Radiation Effects On Cell Adhesionmentioning

confidence: 99%

“…Their study showed that 24 h and 72 h after irradiation the healthy cell line, BALBc/3T3, reduced its speed and motility with both doses of 1 and 2 Gy [ 69 ]. This effect, more evident, was observed at higher doses too, after the delivery of 4 and 8 Gy [ 116 ]. Seventy-two hours after the radiation treatment, BALBc/3T3 cell motility returned to control levels, but only with a dose equal to 1 Gy.…”

Section: Radiation Effects On Cell Migrationmentioning

confidence: 99%

See 1 more Smart Citation

Cytoskeleton Response to Ionizing Radiation: A Brief Review on Adhesion and Migration Effects

et al. 2021

View full text Add to dashboard Cite

The cytoskeleton is involved in several biological processes, including adhesion, motility, and intracellular transport. Alterations in the cytoskeletal components (actin filaments, intermediate filaments, and microtubules) are strictly correlated to several diseases, such as cancer. Furthermore, alterations in the cytoskeletal structure can lead to anomalies in cells’ properties and increase their invasiveness. This review aims to analyse several studies which have examined the alteration of the cell cytoskeleton induced by ionizing radiations. In particular, the radiation effects on the actin cytoskeleton, cell adhesion, and migration have been considered to gain a deeper knowledge of the biophysical properties of the cell. In fact, the results found in the analysed works can not only aid in developing new diagnostic tools but also improve the current cancer treatments.

show abstract

Role of the mechanical microenvironment on CD-44 expression of breast adenocarcinoma in response to radiotherapy

Frascogna,

Mottareale,

La Verde

et al. 2024

Sci Rep

View full text Add to dashboard Cite

The biological effects of ionizing radiation are exploited in the clinical practice of radiotherapy to destroy tumour cells while sparing the surrounding normal tissue. While most of the radiotherapy research focused on DNA damage and repair, recently a great attention is going to cells' interactions with the mechanical microenvironment of both malignant and healthy tissues after exposure. In fact, the stiffness of the extracellular matrix can modify cells' motility and spreading through the modulation of transmembrane proteins and surface receptors' expression, such as CD-44. CD-44 receptor has held much interest also in targeted-therapy due to its affinity with hyaluronic acid, which can be used to functionalize biodegradable nanoparticles loaded with chemotherapy drugs for targeted therapy. We evaluated changes in CD-44 expression in two mammary carcinoma cell lines (MCF10A and MDA-MB-231) after exposure to X-ray (2 or 10 Gy). To explore the role of the mechanical microenvironment, we mimicked tissues' stiffness with polyacrylamide's substrates producing two different elastic modulus values (0.5 and 15 kPa). We measured a dose dependent increase in CD-44 relative expression in tumour cells cultured in a stiffer microenvironment. These findings highlight a crucial connection between the mechanical properties of the cell's surroundings and the post-radiotherapy expression of surface receptors.

show abstract

A Biophysical Analysis to Assess X-Ray Sensitivity of Healthy and Tumour Cells

Cited by 4 publications

References 6 publications

Adhesion and Migration Response to Radiation Therapy of Mammary Epithelial and Adenocarcinoma Cells Interacting with Different Stiffness Substrates

Adhesion and Migration Response to Radiation Therapy of Mammary Epithelial and Adenocarcinoma Cells Interacting with Different Stiffness Substrates

Cytoskeleton Response to Ionizing Radiation: A Brief Review on Adhesion and Migration Effects

Role of the mechanical microenvironment on CD-44 expression of breast adenocarcinoma in response to radiotherapy

Contact Info

Product

Resources

About