A Biomolecular Network Driven Proteinic Interaction in HCV Clearance

Abstract

Hepatitis C virus infection causes chronic liver disease that leads to cancer-related mortality. Presently around 30% of the HCV (infected) affected population get rid of the infection through spontaneous disease clearance. This phenomenon is conducted by a set of reported immune candidate genes. Hence, this study focuses only on these immune-response related genes with aid of network approach, where the idea is to disseminate the network for better understanding of key functional genes and their transcription control activity. Based on the network analysis the IFNG, TNF, IFNB1, STAT1, NFKB1, STAT3, SOCS1, and MYD88 genes are prioritized as hub genes along with their common transcription factors (TFs), IRF9, NFKB1, and STAT1. The dinucleotide frequency of TF binding elements indicated GG-rich motifs in these regulatory elements. On the other hand, gene enrichment report suggests the regulation of response to interferon gamma signaling pathway, which plays central role in the spontaneous HCV clearance. Therefore, our study tends to prioritize the genes, TFs, and their regulatory pathway towards HCV clearance. Even so, the resultant hub genes and their TFs and TF binding elements could be crucial in underscoring the clearance activity in specific populations.

Mechanistic Understanding of the Effect of Temperature and Salinity on the Water/Toluene Interfacial Tension

“…To validate the IFT trends observed in our theoretical studies, we performed experiments to measure the IFTs of VO-79 toluene solution/water binary systems. Several methods exist to measure IFT, including the recently developed survismeter by Singh, 55 which can simultaneously measure surface tension and viscosity of liquids. In this work, IFTs were measured using the pendant drop method with a standard goniometer/tensiometer (ramehart, USA).…”

Philic–phobic chemical dynamics of a 1st tier dendrimer dispersed o/w nanoemulsion

“…Surface tensions and viscosities of the prepared nanoemulsions were estimated by pendant drop number and viscous ow time, respectively, with a Borosil Mansingh Survismeter [24][25][26][27] (calibration no. 06070582/1.01/C-0395, NPL, India) at 293.15 to 313.15 K. The temperature of the survismeter was controlled by a Lauda Alpha KA 8 thermostat to AE0.05 K. The pendant drop number was counted with an electronic counter, while the viscous ow time was recorded with an electronic timer with an accuracy of AE0.1 s. The Survismeter was properly washed with acetone and dried completely before the viscosity and surface tension measurements.…”

Hydrophobics of CnTAB in an aqueous DMSO–BSA nanoemulsion for the monodispersion of flavonoids

“…The drying process was continued until a constant oscillation period for air was attained and noted as an initial calibration. Viscosity (h) and surface tension (g) were measured by using a Borosil Mansingh Survismeter 35 (Cal.no. 06070582/1.01/C-0395, NPL, India) via viscous ow time (VFT) and pendant drop number (PDN) methods.…”