A biomimetic fluorescent nanosensor based on imprinted polymers modified with carbon dots for sensitive detection of alpha-fetoprotein in clinical samples

Sun, Chenghong; Pan, Linli; Zhang, Lei; Huang, Jiaojiao; Yao, Dandan; Wang, Chong‐Zhi; Zhang, Yu; Jiang, Nan; Chen, Lina; Yuan, Chun‐Su

doi:10.1039/c9an01065c

Cited by 56 publications

(14 citation statements)

References 53 publications

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“…The K sv values were estimated to be 0.515 and 0.084 μM −1 for the OM-MIP and OM-NIP NCs, respectively, and hence the IF SV for the OM-NIP NPs was calculated to be 6.13. The limit of detection (LOD) for the OM-MIP NCs were determined to be 38.1 nM, according to 3 σ / s , 52–57 where σ means the standard deviation of the blank measurements ( n = 11) and s the slope of calibration curve ( s = K sv ). For comparison, the FL spectra of the OM-MIP and OM-NIP NCs without PEGylation were also measured (Fig.…”

Section: Resultsmentioning

confidence: 99%

Controlled synthesis of open-mouthed epitope-imprinted polymer nanocapsules with a PEGylated nanocore and their application for fluorescence detection of target protein

Feng

Jin

et al. 2022

RSC Adv.

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Section: Resultsmentioning

confidence: 99%

Controlled synthesis of open-mouthed epitope-imprinted polymer nanocapsules with a PEGylated nanocore and their application for fluorescence detection of target protein

Feng

Jin

et al. 2022

RSC Adv.

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“…Moreover, the responsive DNA hydrogel SERS biosensing platform was compared with other methods of AFP detection (Table ). − In terms of detection limit, this SERS platform covered the range of AFP concentration in serum from healthy adults to patients with advanced liver cancer (25 ng/mL to 500 ng/mL). Compared with other detection methods, it has the advantages of wider linear range, lower cost, and good reproducibility.…”

Section: Resultsmentioning

confidence: 99%

Preparation of Aptamer Responsive DNA Functionalized Hydrogels for the Sensitive Detection of α-Fetoprotein Using SERS Method

Wang

Jiang

et al. 2020

Bioconjugate Chem.

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Hepatocellular carcinoma (HCC), which is one of the three major cancers, has attracted growing attention due to its high mortality, health care cost, and circumscribed therapeutic methods. Hence, the development of a fast, accurate, and flexible method to detect α-fetoprotein (AFP), the specific marker of HCC, is significant for diagnosis and treatment of cancer. Here, we constructed a novel SERS biosensing platform combining the target-responsive DNA hydrogel for the sensitive detection of AFP. The linker strand in DNA hydrogel is an aptamer that can specifically recognize AFP and accurately control the release of immunoglobulin G (IgG) encapsulated in hydrogel. In the presence of AFP, the hydrogels were disentangled and the IgG was released. Thereafter, the released IgG was captured by SERS probes and biofunctional magnetic beads through formation of sandwich-like structures, resulting in the signal of Raman tags decreasing in the supernatant after magnetic separation. Due to the ultrahigh sensitivity of the SERS biosensor, the proposed method has a wide detection linear range (50 pg/mL to 0.5 μg/mL) and a detection limit down to 50 pg/mL. Moreover, the sequence of the linker strand in the DNA hydrogel can be specifically encoded into a new aptamer that responds to other cancer markers. This convenient and inexpensive detection method provides a new strategy for the detection of tumor markers.

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“…2019 described that for the detection of alpha‐fetoprotein (AFP) a biomimetic fluorescent nanosensor based on molecularly imprinted polymers modified with CDs (CDs@MIPs) can be used. Moreover, for the rapid, selective, and sensitive detection of tumor markers, CDs@MIPs were turned out to be a success to detect AFP in human serum samples [107] . Nano light CDs were synthesized with high fluorescence properties, and used for labeling antibodies via amine‐amine conjugations.…”

Section: Applications Of Carbon Dotsmentioning

confidence: 99%

Recent Development in Synthesis of Carbon Dots from Natural Resources and Their Applications in Biomedicine and Multi‐Sensing Platform

et al. 2021

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Carbon has always found a spotlight in human life either in the form of graphite, diamonds, activated charcoal, carbon nanotubes, or carbon dots (CDs). Carbon quantum dots (CQDs) or CDs became the centre of attraction because of their potential applications in biomedical field such as bio‐imaging, drug nanocarriers, gene therapeutics, photosensitizing agents, theranostics and provides other multifunctional diagnostic platforms. There are numerous advantages of CDs due to their biocompatibility, reduced toxicity, water‐solubility, ease of scale‐up, and, most notably their fluorescence properties contrary to other carbon nanostructures. In last few years, significant efforts have been put by various research teams to synthesize and develop nanostructured materials possessing specific functional properties and characteristics. This work focus on the recent developments in the synthetic approaches applied for CDs using environment‐friendly sources. This review also highlights the mechanistic aspects, applications of CDs in biomedicine and a multi‐sensing platform along with challenges and future perspectives of this promising field. The authors believe that this review will help audible researchers to select a suitable environment‐friendly approach for the synthesis of CDs from green sources with required functions and properties. It will also guide researchers to develop a more convenient, efficient, and environment‐friendly method for the synthesis of new tunable carbon dots.

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A biomimetic fluorescent nanosensor based on imprinted polymers modified with carbon dots for sensitive detection of alpha-fetoprotein in clinical samples

Abstract: A biomimetic fluorescent nanosensor based on molecularly imprinted polymers modified with carbon dots (CDs@MIPs) has been prepared for rapid, selective and sensitive detection of alpha-fetoprotein (AFP) in clinical samples.

Cited by 56 publications

References 53 publications

Controlled synthesis of open-mouthed epitope-imprinted polymer nanocapsules with a PEGylated nanocore and their application for fluorescence detection of target protein

Controlled synthesis of open-mouthed epitope-imprinted polymer nanocapsules with a PEGylated nanocore and their application for fluorescence detection of target protein

Preparation of Aptamer Responsive DNA Functionalized Hydrogels for the Sensitive Detection of α-Fetoprotein Using SERS Method

Recent Development in Synthesis of Carbon Dots from Natural Resources and Their Applications in Biomedicine and Multi‐Sensing Platform

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