A bioluminescence assay for total 3α-hydroxy bile acids in serum using immobilized enzymes

Schöelmerich, Juergen; Henegouwen, G. P. van Berge; Hofmann, Alan F.; DeLuca, Marlene

doi:10.1016/0009-8981(84)90308-5

Cited by 31 publications

(8 citation statements)

References 56 publications

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“…Biochemical parameters of liver function [serum bilirubin, alkaline phosphatase (AP), gamma‐GT, AST, ALT, glutamate‐dehydrogenase, cholinesterase, albumin, prothrombin time, plasma ammonia] as well as full blood count and other routine laboratory parameters (serum total protein, C‐reactive protein, lactate dehydrogenase, amylase, sodium, potassium, creatinine, urea, uric acid, glucose, lactate, cholesterol, triglycerides) were measured twice a week. Total bile acids were measured enzymatically once per week as described elsewhere 4 …”

Section: Methodsmentioning

confidence: 99%

Repeated administration of a vitamin preparation containing glycocholic acid in patients with hepatobiliary disease

Rifai

Ockenga

Manns

et al. 2006

Aliment Pharmacol Ther

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SUMMARY BackgroundUntil now, hydrophilic and lipophilic vitamin preparations had to be administered separately during total parenteral nutrition. By addition of glycocholic acid, a vitamin supplement (Cernevit, Baxter, Heidelberg, Germany) was developed that combines all vitamins into one vial. However, little information exists about possible consequences of bile acid administration such as glycocholic acid, especially if liver disease is pre-existing.

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Section: Methodsmentioning

confidence: 99%

Repeated administration of a vitamin preparation containing glycocholic acid in patients with hepatobiliary disease

Rifai

Ockenga

Manns

et al. 2006

Aliment Pharmacol Ther

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“…The remaining enzymes, which were not commercially available, were supplied by Dr. Marlene DeLuca, Dep. of Chemistry, University of California, San Diego, CA (USA) (bacterial luciferase) [27] and by Dr. Ian A. MacDonald, Dep. of Medicine, Dalhousie University, Halifax, Canada (12a-hydroxysteroid dehydrogenase) [26].…”

Section: Methodsmentioning

confidence: 99%

“…Bile samples were weighed and then diluted (1 : 10-1 : 20). Bile acid concentrations were analyzed using bioluminescence assays for 3ct-OH-, 7u-OH-, and 12ct-OH-bile acids which have been published recently [23,26,27]. Functional parameters of forward and retrograde perfusion groups were compared using the Student's t-test.…”

Section: Methodsmentioning

confidence: 99%

Structural and functional integrity of rat liver perfused in backward and forward directions

1986

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The purpose of this study was to assess if reversal of the direction of isolated rat liver perfusion would cause significant alterations in hepatic functions and structure. Five isolated rat livers were perfused forward and another five backward with oxygenated Ringer's solution for up to 90 min (hydrostatic pressure: less than or equal to 13 cm H2O; flow rate: forward 3.88 +/- 0.34 ml/min per gram and backward 3.76 +/- 0.34 ml/min per gram). At the end of the experiment, livers were perfusion-fixed for morphological examination. The following results were obtained: No significant differences were noted between the forward and backward perfusions with respect to oxygen uptake, mean bile flow (forward 0.57 +/- 0.12; backward 0.60 +/- 0.14 ml/min per gram), average bile acid excretion (forward 2.39 +/- 1.11; backward 2.83 +/- 0.94 nmol/min per gram), hydroxylation pattern of bile acids, urea synthesis, release of lactic dehydrogenase, glucose secretion, and redox ratios. Light and electron microscopy, including morphometry of parenchymal and sinusoidal areas, revealed that the backward perfusion caused a greater degree of sinusoidal distension, but no other noteworthy differences. Hepatic ultrastructure was well preserved. We conclude that reversing the direction of perfusion does not alter structure and major hepatic functions significantly.

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“…73 The enzymatic measurement of total 3␣-hydroxy bile acids was a Japanese import and was based on the pioneering studies on 3␣-hydroxysteroid dehydrogenase by Paul Talalay at the Johns Hopkins University, my alma mater. With Aldo Roda, Juergen Schoelmerich, and the late Marlene DeLuca, we converted the enzymatic measurement of bile acids into a bioluminescence technique for measuring 3␣-hydroxy 74 and 7␣-hydroxy 75 bile acids. We used this highly sensitive technique to show that CDCA was well absorbed, 76 as well as to examine changes in the postprandial elevation of bile acids during bile acid sequestrant administration.…”

Section: Quantifying Bile Acidsmentioning

confidence: 99%

Bile acids: Trying to understand their chemistry and biology with the hope of helping patients

2008

Self Cite

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An informal review of the author's five decades of research on the chemistry and biology of bile acids in health and disease is presented. The review begins with a discussion of bile acid structure and its remarkable diversity in vertebrates. Methods for tagging bile acids with tritium for metabolic or transport studies are summarized. Bile acids solubilize polar lipids in mixed micelles; progress in elucidating the structure of the mixed micelle is discussed. Extensive studies on bile acid metabolism in humans have permitted the development of physiological pharmacokinetic models that can be used to simulate bile acid metabolism. Consequences of defective bile acid biosynthesis and transport have been clarified, and therapy has been developed. Methods for measuring bile acids have been improved. The rise and fall of medical and contact dissolution of cholesterol gallstones is chronicled. Finally, principles of therapy with bile acid agonists and antagonists are given. Advances in understanding bile acid biology and chemistry have helped to improve the lives of patients with hepatobiliary or digestive disease.

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A bioluminescence assay for total 3α-hydroxy bile acids in serum using immobilized enzymes

Cited by 31 publications

References 56 publications

Repeated administration of a vitamin preparation containing glycocholic acid in patients with hepatobiliary disease

Repeated administration of a vitamin preparation containing glycocholic acid in patients with hepatobiliary disease

Structural and functional integrity of rat liver perfused in backward and forward directions

Bile acids: Trying to understand their chemistry and biology with the hope of helping patients

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