A Biological OR(XNOR) Logic Gate Couples Carbon Source and Transgene Expression Switching in a Komagataella phaffii (Pichia pastoris) Strain Co-producing Process-Enhancing Lipase and a Virus-like Particle (VLP) Vaccine
Abstract:We constructed a three-input biological logic gate: S
OR (G XNOR
M), where S is sorbitol, G is glycerol, and M is methanol, to optimize
co-expression of two transgenes in Komagataella phaffii using batch-mode carbon source switching (CSS). K. phaffii was engineered to harbor transgenes encoding a Candida rugosa triacylglycerol lipase, which can enhance downstream processing
by removing host cell lipids from homogenates, and the hepatitis B
virus surface antigen (HBsAg), a protein that self-assembles into
a vir… Show more
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