A biogenic microbial biosurfactin that degrades difenoconazole fungicide with potential antimicrobial and oil displacement properties

Satapute, Praveen; Jogaiah, Sudisha

doi:10.1016/j.chemosphere.2021.131694

Cited by 18 publications

(4 citation statements)

References 38 publications

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“…OF-1, produces an anti-pneumococcal compound of proteinaceous nature that we named lysinicin OF. A few previous studies have reported that members of the genus Lysinibacillus produce antimicrobials of proteinaceous nature such as heat-labile bacteriocins (inactivated >80 °C) and lipopeptides [59–61]. Since lysinicin OF could resist both heat (100 °C) and polymyxin acylase treatment (cleaves off the peptide part of lipopeptides), we concluded that it most probably is a peptide.…”

Section: Discussionmentioning

confidence: 79%

A novel proteinaceous molecule produced by Lysinibacillus sp. OF-1 depends on the Ami oligopeptide transporter to kill Streptococcus pneumoniae

et al. 2023

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Infections caused by antibiotic-resistant Streptococcus pneumoniae are of growing concern for healthcare systems, which need new treatment options. Screening microorganisms in terrestrial environments has proved successful for discovering antibiotics, while production of antimicrobials by marine microorganisms remains underexplored. Here we have screened microorganisms sampled from the Oslo Fjord in Norway for production of molecules that prevent the human pathogen S. pneumoniae from growing. A bacterium belonging to the genus Lysinibacillus was identified. We show that this bacterium produces a molecule that kills a wide range of streptococcal species. Genome mining in BAGEL4 and AntiSmash suggested that it was a new antimicrobial compound, and we therefore named it lysinicin OF. The compound was resistant to heat (100 °C) and polymyxin acylase but susceptible to proteinase K, showing that it is of proteinaceous nature, but most probably not a lipopeptide. S. pneumoniae became resistant to lysinicin OF by obtaining suppressor mutations in the ami locus, which encodes the AmiACDEF oligo peptide transporter. We created ΔamiC and ΔamiEF mutants to show that pneumococci expressing a compromised Ami system were resistant to lysinicin OF. Furthermore, by creating mutants expressing an intact but inactive Ami system (AmiED184A and AmiFD175A) we could conclude that the lysinicin OF activity depended on the active form (ATP-hydrolysing) of the Ami system. Microscopic imaging and fluorescent labelling of DNA showed that S. pneumoniae treated with lysinicin OF had an average reduced cell size with condensed DNA nucleoid, while the integrity of the cell membrane remained intact. The characteristics and possible mode of action of lysinicin OF are discussed.

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Section: Discussionmentioning

confidence: 79%

A novel proteinaceous molecule produced by Lysinibacillus sp. OF-1 depends on the Ami oligopeptide transporter to kill Streptococcus pneumoniae

et al. 2023

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“…Similarly, Lysinibacillus sp. BTKU3 also exhibited the ability to degrade difenoconazole at a concentration of 9.1 μg/ml within 3 days [ 53 ]. Meanwhile, Burkholderia sp.…”

Section: Resultsmentioning

confidence: 99%

Degradation of Triazole Fungicides by Plant Growth-Promoting Bacteria from Contaminated Agricultural Soil

Luong,

Nguyen,

Nguyen

et al. 2023

J. Microbiol. Biotechnol.

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The widespread application of triazole fungicides (TFs) in agricultural practices can result in the considerable accumulation of active compound residues in the soil and a subsequent negative impact on the soil microbiota and crop health. In this study, we isolated three TF-degrading bacterial strains from contaminated agricultural soils and identified them as Klebsiella sp., Pseudomonas sp., and Citrobacter sp. based on analysis of morphological characteristics and 16S rRNA gene sequences. The strains used three common TFs, namely hexaconazole, difenoconazole, and propiconazole, as their only sources of carbon and energy for growth in a liquid mineral salt medium, with high concentrations (~ 500 mg/l) of each TF. In addition to the ability to degrade fungicides, the isolates also exhibited plant growth-promoting characteristics, such as nitrogen fixation, indole acetic acid production, phosphate dissolution, and cellulose degradation. The synergistic combination of three bacterial isolates significantly improved plant growth and development with an increased survival rate (57%), and achieved TF degradation ranging from 85.83 to 96.59% at a concentration of approximately 50 mg/kg of each TF within 45 days in the soil-plant system. Based on these findings, the three strains and their microbial consortium show promise for application in biofertilizers, to improve soil health and facilitate optimal plant growth.

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“…Another study showed a 76% decrease in COD after 72 h of incubation by the Planococcus citreus strain and a 70% reduction by Pantoea agglomerans with an initial COD value of 4400 mg/L [65]. Multiple studies have also tested different hydrocarbon-degrading strains for the antimicrobial activity of the biosurfactant, which showed a positive result; hence, both of these strains can be further tested to increase their application in bioremediation [66][67][68]. Our isolated strains (P. aeruginosa + B. subtilis) used in a 1:1 ratio for power generation were capable enough to enhance power production.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Biosurfactant-Producing Bacteria and Their Co-Culture Application in Microbial Fuel Cell for Simultaneous Hydrocarbon Degradation and Power Generation

et al. 2022

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Biosurfactant-producing microorganisms improve the efficacy of hydrocarbon biodegradation as the biosurfactant is essential in making hydrocarbons available for breakdown. The present study reports the isolation of biosurfactant-producing bacteria that can be used for crude oil remediation and to characterize the biosurfactant generated during the breakdown of crude oil. This study also reports evaluating the synergism and potentiality of biosurfactant-producing bacteria for simultaneous hydrocarbon biodegradation and power generation. Two bacterial strains (Bacillus subtilis strain B1 and Pseudomonas aeruginosa strain B2) were isolated from petroleum-contaminated soils, which are found effective in producing biosurfactants and degrading crude oil as the sole carbon source. B. subtilis B1 exhibited a higher potential for biosurfactant production and crude oil degradation than P. aeruginosa B2. The FTIR and GC-MS analysis were conducted for further characterization of the biosurfactant, which revealed that the surfactant produced by strain B1 and B2 was surfactin and rhamnolipid, respectively. The application of the B1 and B2 co-culture in microbial fuel cells (MFCs) showed synergism among them and resulted in a maximum power density production of 6.3 W/m3 with an open circuit voltage of 970 mV while degrading 2.5% v/v crude oil containing anolyte. The findings indicate that the co-culture of isolated crude oil-degrading strains has great potential for enhanced power generation and the bioremediation of hydrocarbon-contaminated environments. Moreover, the synergism of isolated strains in MFCs suggested their potent applicability in environmental, energy, and industrial sectors as an economical and feasible alternative to the existing technologies.

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A biogenic microbial biosurfactin that degrades difenoconazole fungicide with potential antimicrobial and oil displacement properties

Cited by 18 publications

References 38 publications

A novel proteinaceous molecule produced by Lysinibacillus sp. OF-1 depends on the Ami oligopeptide transporter to kill Streptococcus pneumoniae

A novel proteinaceous molecule produced by Lysinibacillus sp. OF-1 depends on the Ami oligopeptide transporter to kill Streptococcus pneumoniae

Degradation of Triazole Fungicides by Plant Growth-Promoting Bacteria from Contaminated Agricultural Soil

Isolation of Biosurfactant-Producing Bacteria and Their Co-Culture Application in Microbial Fuel Cell for Simultaneous Hydrocarbon Degradation and Power Generation

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