A bioengineering perspective on modelling the intestinal epithelial physiology in vitro

Antfolk, Maria; Jensen, Kim B.

doi:10.1038/s41467-020-20052-z

Cited by 27 publications

(17 citation statements)

References 116 publications

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“…In vivo, the intestinal epithelium rests on a supporting basement membrane composed of structural and adhesive proteins that epithelial cells use to anchor, migrate, and differentiate ( 34 ). Matrix properties such as ECM ligands, stiffness, and porosity are key factors that influence a wide range of cell behaviors including viability, tissue organization/architecture, and stem cell renewal and differentiation ( 35 , 36 ).…”

Section: Resultsmentioning

confidence: 99%

Innate immune cell response to host-parasite interaction in a human intestinal tissue microphysiological system

et al. 2022

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Protozoan parasites that infect humans are widespread and lead to varied clinical manifestations, including life-threatening illnesses in immunocompromised individuals. Animal models have provided insight into innate immunity against parasitic infections; however, species-specific differences and complexity of innate immune responses make translation to humans challenging. Thus, there is a need for in vitro systems that can elucidate mechanisms of immune control and parasite dissemination. We have developed a human microphysiological system of intestinal tissue to evaluate parasite-immune–specific interactions during infection, which integrates primary intestinal epithelial cells and immune cells to investigate the role of innate immune cells during epithelial infection by the protozoan parasite, Toxoplasma gondii , which affects billions of people worldwide. Our data indicate that epithelial infection by parasites stimulates a broad range of effector functions in neutrophils and natural killer cell–mediated cytokine production that play immunomodulatory roles, demonstrating the potential of our system for advancing the study of human-parasite interactions.

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Section: Resultsmentioning

confidence: 99%

Innate immune cell response to host-parasite interaction in a human intestinal tissue microphysiological system

et al. 2022

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“…In vivo , the intestinal epithelium rests on a supporting basement membrane composed of structural and adhesive proteins that epithelial cells use to anchor, migrate, and differentiate [54]. Matrix properties such as ECM ligands, stiffness and porosity are key factors that influence a wide range of cell behaviors including viability, tissue organization/architecture, and stem cell renewal and differentiation [55–58].…”

Section: Resultsmentioning

confidence: 99%

Innate immune cell response to host-parasite interaction in a human intestinal tissue microphysiological system

Humayun

Ayuso

Park

et al. 2022

Preprint

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Protozoan parasites that infect humans are widespread, and lead to varied clinical manifestations, including life-threatening illnesses in immunocompromised individuals. Animal models have provided insight into innate immunity against parasitic infections, however, species-specific differences and complexity of innate immune responses make translation to humans challenging. Thus, there is a need for novel in vitro systems that can elucidate mechanisms of immune control and parasite dissemination. We have developed a human microphysiological system of intestinal tissue to evaluate parasite-immune-specific interactions during infection, which integrates primary intestinal epithelial cells and immune cells to investigate the role of innate immune cells during epithelial infection by the protozoan parasite, Toxoplasma gondii, which affects billions of people worldwide. Our data indicate that epithelial-infection by parasites stimulates a broad range of effector functions in neutrophils and NK cell-mediated cytokine production that play immunomodulatory roles, demonstrating the potential of our system for advancing the study of human-parasite interactions.

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“…Therefore, the changes in the structure and function of the small intestinal mucosa are closely related to the digestion and absorption of nutrients. Moreover, the villi, finger-like protrusions of the small intestine expand the surface area of the mucosa and are arranged within the intestinal mucosa epithelial cell layer facing the lumen to form a protective barrier to protect the body from direct contact with microorganisms and food antigens [ 29 ]. ZEA can cause intestinal damage, which would lead to the impairment of the intestinal absorption capacity and barrier function [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Zearalenone Affect the Intestinal Villi Associated with the Distribution and the Expression of Ghrelin and Proliferating Cell Nuclear Antigen in Weaned Gilts

Zhang

Huang

Leng

et al. 2021

Toxins

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This study explored and investigated how zearalenone (ZEA) affects the morphology of small intestine and the distribution and expression of ghrelin and proliferating cell nuclear antigen (PCNA) in the small intestine of weaned gilts. A total of 20 weaned gilts (42-day-old, D × L × Y, weighing 12.84 ± 0.26 kg) were divided into the control and ZEA groups (ZEA at 1.04 mg/kg in diet) in a 35-d study. Histological observations of the small intestines revealed that villus injuries of the duodenum, jejunum and ileum, such as atrophy, retardation and branching dysfunction, were observed in the ZEA treatment. The villi branch of the ileum in the ZEA group was obviously decreased compared to that of the ileum, jejunum and duodenum, and the number of lymphoid nodules of the ileum was increased. Additionally, the effect of ZEA (1.04 mg/kg) was decreased by the immunoreactivity and distribution of ghrelin and PCNA in the duodenal and jejunal mucosal epithelial cells. Interestingly, ZEA increased the immunoreactivity of ghrelin in the ileal mucosal epithelial cells and decreased the immunoreactivity expression of PCNA in the gland epithelium of the small intestine. In conclusion, ZEA (1.04 mg/kg) had adverse effects on the development and the absorptive capacity of the villi of the intestines; yet, the small intestine could resist or ameliorate the adverse effects of ZEA by changing the autocrine of ghrelin in intestinal epithelial cells.

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A bioengineering perspective on modelling the intestinal epithelial physiology in vitro

Cited by 27 publications

References 116 publications

Innate immune cell response to host-parasite interaction in a human intestinal tissue microphysiological system

Innate immune cell response to host-parasite interaction in a human intestinal tissue microphysiological system

Innate immune cell response to host-parasite interaction in a human intestinal tissue microphysiological system

Zearalenone Affect the Intestinal Villi Associated with the Distribution and the Expression of Ghrelin and Proliferating Cell Nuclear Antigen in Weaned Gilts

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