A biodegradable hybrid inorganic nanoscaffold for advanced stem cell therapy

Abstract

Stem cell transplantation, as a promising treatment for central nervous system (CNS) diseases, has been hampered by crucial issues such as a low cell survival rate, incomplete differentiation, and limited neurite outgrowth in vivo. Addressing these hurdles, scientists have designed bioscaffolds that mimic the natural tissue microenvironment to deliver physical and soluble cues. However, several significant obstacles including burst release of drugs, insufficient cellular adhesion support, and slow scaffold degradation rate remain to be overcome before the full potential of bioscaffold–based stem-cell therapies can be realized. To this end, we developed a biodegradable nanoscaffold-based method for enhanced stem cell transplantation, differentiation, and drug delivery. These findings collectively support the therapeutic potential of our biodegradable hybrid inorganic (BHI) nanoscaffolds for advanced stem cell transplantation and neural tissue engineering.

Effective Modulation of CNS Inhibitory Microenvironment using Bioinspired Hybrid‐Nanoscaffold‐Based Therapeutic Interventions

“…2D nanomaterial (e.g., graphene)-based hybrid scaffolds developed by our group, as well as others, have shown excellent protein absorption with a high surface-area-tovolume ratio, drug loading, and bioimaging properties that offer clear advantages over conventional bioscaffolds. [8,14] However, currently, there is a lack of reliable methods to produce the desired biomimetic ordered 3D porous structures from biodegradable 2D MnO 2 nanomaterials without compromising their chemical stability, biocompatibility, and bioactivity. [8,9,15,16] Inspired by a conventional electrostatic LBL technique as well as recent advancement of diffusion-driven 3D assembly of graphene nanosheets, we developed a synthetic route to generate our 3D-BPH nanoscaffolds from a biodegradable 2D nanomaterial (i.e., MnO 2 nanosheets) and a US Food and Drug Administration (FDA)approved cationic polymer (i.e., chitosan), as a means to provide a clinically-relevant nanomaterial-based bioscaffold (Figure 2a).…”

“…[8,14] However, currently, there is a lack of reliable methods to produce the desired biomimetic ordered 3D porous structures from biodegradable 2D MnO 2 nanomaterials without compromising their chemical stability, biocompatibility, and bioactivity. [8,9,15,16] Inspired by a conventional electrostatic LBL technique as well as recent advancement of diffusion-driven 3D assembly of graphene nanosheets, we developed a synthetic route to generate our 3D-BPH nanoscaffolds from a biodegradable 2D nanomaterial (i.e., MnO 2 nanosheets) and a US Food and Drug Administration (FDA)approved cationic polymer (i.e., chitosan), as a means to provide a clinically-relevant nanomaterial-based bioscaffold (Figure 2a). [17] More specifically, 2D-MnO 2 nanosheets were first generated through liquid exfoliation ( Figure S1, Supporting Information).…”

Wearable and Implantable Devices for Cardiovascular Healthcare: from Monitoring to Therapy Based on Flexible and Stretchable Electronics

“…Previous electronic scaffolds, however, consisted of nondegradable synthetic materials such as SU‐8 or PI, and an additional surgical procedure is inevitable to remove the synthetic scaffold . Therefore, an electronic scaffold that can degrade in vivo is needed . The Au electrodes were fabricated onto an electrospun albumin scaffold, which serves as a substrate and passivation layer, to construct engineered cardiac tissue (Figure g; the inset shows an image of a rolled scaffold) .…”

Design Parameters of Tissue‐Engineering Scaffolds at the Atomic Scale

“…Stem cells respond to their microenvironment, which provides critical cues for their migration, differentiation, and proliferation . The regulatory interplay between stem cells and their native extracellular matrix has important implications for the design of biomimetic tissue‐engineering scaffolds, with potentially exciting applications in regenerative medicine that could lead to a paradigm shift in therapeutic treatments . In order to improve our capabilities in engineering tissues, in‐depth understanding of properties of stem cell substrates is essential .…”