A biodegradable device for the controlled release of Piper nigrum (Piperaceae) standardized extract to control Aedes aegypti (Diptera, Culicidae) larvae

Abstract

Introduction:The signifi cant increase in dengue, Zika, and chikungunya and the resistance of the Aedes aegypti mosquito to major insecticides emphasize the importance of studying alternatives to control this vector. The aim of this study was to develop a controlled-release device containing Piper nigrum extract and to study its larvicidal activity against Aedes aegypti. Methods: Piper nigrum extract was produced by maceration, standardized in piperine, and incorporated into cotton threads, which were inserted into hydrogel cylinders manufactured by the extrusion of carrageenan and carob. The piperine content of the extract and thread reservoirs was quantifi ed by chromatography. The release profi le from the device was assessed in aqueous medium and the larvicidal and residual activities of the standardized extract as well as of the controlled-release device were examined in Aedes aegypti larvae. Results: The standardized extract contained 580mg/g of piperine and an LC 50 value of 5.35ppm (24h) and the 3 cm thread reservoirs contained 13.83 ± 1.81mg of piperine. The device showed zero-order release of piperine for 16 days. The P. nigrum extract (25ppm) showed maximum residual larvicidal activity for 10 days, decreasing progressively thereafter. The device had a residual larvicidal activity for up to 37 days. Conclusions: The device provided controlled release of Piper nigrum extract with residual activity for 37 days. The device is easy to manufacture and may represent an effective alternative for the control of Aedes aegypti larvae in small water containers.

Edible Plant Extracts against Aedes aegypti and Validation of a Piper nigrum L. Ethanolic Extract as a Natural Insecticide

“…The LC 50 and LC 90 values suggest that larvae mortality is related to the combination of piperine concentration, and the minority compounds detected ( Figure 4 B,C). A previous study [ 26 ] correlating the quality control of P. nigrum samples and Ae. aegypti larvae did not include all the parameters validated herein.…”

Phytochemical Analysis and Determination of the Chemical Composition of Larvicidal Extracts of Black Pepper (Piper nigrum L.) Waste: An Undergraduate Chemistry Experiment

“…Following characterization of P. nigrum extracts, students were encouraged to identify, through a literature search, the biological activities of the putatively identified compounds. Most students found scientific articles demonstrating the potential of piperamides in killing larvae of disease-carrying mosquitoes. − The students also found studies showing that P. nigrum extracts exert larvicidal effects on A. aegypti . − The students suggested investigating the potential of hexane and dichloromethane extracts to kill A. aegypti larvae because of the importance of dengue in Brazil (1,544,987 cases in 2019) and the need for developing new biodegradable alternatives to control the disease.…”

Acaricidal activity of extracts from different structures of Piper tuberculatum against larvae and adults of Rhipicephalus microplus

“…The promising biocidal activity of various plants to control different pathogenic vectors and agents that impair livestock and human health has been demonstrated by various studies (Klauck et al 2014;Custódio et al 2016;Rodrigues et al 2017;Fatemi et al 2017). The active research interest in the chemical components produced by the metabolism of plants has led to the isolation of various substances that take part in the defense mechanisms of plants against attack by pests and diseases.…”