Contamination of meat with pathogenic microorganisms
can cause
severe illnesses and food waste, which has significant negative impacts
on both general health and the economy. In many cases, the expiration
date is not a good indicator of meat freshness as there is a high
risk of contamination during handling throughout the supply chain.
Many biomarkers, including color, odor, pH, temperature, and volatile
compounds, are used to determine spoilage. Among these, pH presents
a simple and effective biomarker directly linked to the overgrowth
of bacteria and degradation of the meat tissue. Low-cost methods for
wireless pH monitoring are crucial in detecting spoilage on a large
commercial scale. Existing technologies are often limited to short-range
detection, with the use of batteries and different electronic components
that increases both the manufacturing complexity and cost of the final
device. To address these shortcomings, we have developed a cost-effective
wireless pH sensor, which uses passive resonant frequency (RF) sensing,
combined with a pH-responsive polymer that can be placed within packaged
meat products and provide a remote assessment of the risk of microbial
spoilage throughout the supply chain. The sensor tag consists of a
sensing resonator coated with a pH-sensitive material and a passivated
reference resonator operating in a differential frequency configuration.
Upon exposure to elevated pH levels >6.8, the coating on the sensing
resonator dissolves, which in turn results in a distinct change in
the resonant frequency with respect to the reference resonator. Systematic
theoretical and experimental results at different pH levels demonstrated
that a 20% shift in resonant frequency demarcates the point for spoilage
detection. As a proof of concept, the performance of the sensor in
remotely detecting the risk of food spoilage was validated in packaged
poultry over 10 days. The sensor fabrication process takes advantage
of recent developments in the scalable manufacturing of flexible,
low-cost devices, including selective laser etching of metalized plastic
films and doctor-blade coating of stimuli-responsive polymer films.
Furthermore, the biocompatibility of all the materials used in the
sensor was confirmed with human intestinal cells (HCT-8 cells).