A biocornea of fish scales – First results of a research model

Essen, Th Van; Lin, Ching Chun; Lai, HJ; Hussain, Ak; Luyten, Gpm; Jager, M. J.

doi:10.1111/j.1755-3768.2011.2376.x

Cited by 3 publications

(4 citation statements)

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“…The FSS is primarily built of collagen type I, similar to the corneal stroma, so the attachment of the scaffold is expected to happen similarly. Also, transparency of FSS was acceptable and it did not degrade in vivo in any of the previously published studies [ 8 – 10 ].…”

Section: Discussionmentioning

confidence: 71%

“…Moreover, its transparency and availability, that is, roughly 200 scales from one fish, make it an attractive biocompatible material for the generation of corneal epithelial cell grafts. Additional in vivo studies performed on rats and rabbits have demonstrated its potential as a deep anterior lamellar keratoplasty (DALK) alternative or to seal perforated corneas, respectively [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Fish Scale-Derived Scaffolds for Culturing Human Corneal Endothelial Cells

Parekh

Bogerd

Zakaria

et al. 2018

Stem Cells International

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Purpose To investigate the biocompatibility of fish scale-derived scaffolds (FSS) with primary human corneal endothelial cells (HCEnCs). Methods HCEnCs were isolated from 30 donor corneas in a donor-matched study and plated in precoated Lab-Tek slides (n = 15) and FSS (n = 15). Cell morphology, proliferation/migration, and glucose uptake were studied (n = 30). Hoechst, ethidium homodimer, and calcein AM (HEC) staining was performed to determine viability and toxicity (n = 6). The cell surface area was calculated based on calcein AM staining. HCEnCs were stained for ZO-1 (n = 6) to detect tight junctions and to measure cell morphology; Ki-67 (n = 6) to measure proliferating cells; and vinculin to quantify focal adhesions (n = 6). The formation of de novo extracellular matrix was analyzed using histology (n = 6). Results HCEnCs attach and grow faster on Lab-Tek slides compared to the undulating topography of the FSS. At day 11, HCEnCs on Lab-Tek slide grew 100% confluent, while FSS was only 65% confluent (p = 0.0883), with no significant difference in glucose uptake between the two (p = 0.5181) (2.2 μg/mL in Lab-Tek versus 2.05 μg/mL in FSS). HEC staining showed no toxicity. The surface area of the cells in Lab-Tek was 409.1 μm2 compared to 452.2 μm2 on FSS, which was not significant (p = 0.5325). ZO-1 showed the presence of tight junctions in both conditions; however, hexagonality was higher (74% in Lab-Tek versus 45% in FSS; p = 0.0006) with significantly less polymorphic cells on Lab-Tek slides (8% in Lab-Tek versus 16% in FSS; p = 0.0041). Proliferative cells were detected in both conditions (4.6% in Lab-Tek versus 4.2% in FSS; p = 0.5922). Vinculin expression was marginally higher in HCEnCs cultured on Lab-Tek (234 versus 199 focal adhesions; p = 0.0507). Histological analysis did not show the formation of a basement membrane. Conclusions HCEnCs cultured on precoated FSS form a monolayer, displaying correct morphology, cytocompatibility, and absence of toxicity. FSS needs further modification in terms of structure and surface chemistry before considering it as a potential carrier for cultured HCEnCs.

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Section: Discussionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Fish Scale-Derived Scaffolds for Culturing Human Corneal Endothelial Cells

Parekh

Bogerd

Zakaria

et al. 2018

Stem Cells International

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“…Additional in vivo studies performed on rats and rabbits have demonstrated its potential as a deep anterior lamellar keratoplasty (DALK) alternative or to seal perforated corneas, respectively. 61 However, a recent report suggested that the fish scale derived scaffold in its current form may not be ideal for the development of tissue engineered corneal endothelial constructs. 62 Although hCECs adhered to the surface of the scales, regional differences in cellular proliferation were observed.…”

Section: Biologically Derived Materialsmentioning

confidence: 99%

Biomaterials for corneal endothelial cell culture and tissue engineering

et al. 2021

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The corneal endothelium is the posterior monolayer of cells that are responsible for maintaining overall transparency of the avascular corneal tissue via pump function. These cells are non-regenerative in vivo and therefore, approximately 40% of corneal transplants undertaken worldwide are a result of damage or dysfunction of endothelial cells. The number of available corneal donor tissues is limited worldwide, hence, cultivation of human corneal endothelial cells (hCECs) in vitro has been attempted in order to produce tissue engineered corneal endothelial grafts. Researchers have attempted to recreate the current gold standard treatment of replacing the endothelial layer with accompanying Descemet’s membrane or a small portion of stroma as support with tissue engineering strategies using various substrates of both biologically derived and synthetic origin. Here we review the potential biomaterials that are currently in development to support the transplantation of a cultured monolayer of hCECs.

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“…The substitute is cell-free and of high biocompatibility with host tissue to restore corneal function, thereby avoiding the rejection reaction and the need for long-term steroid use. van Essen et al [ 23 ] demonstrated safety, biocompatibility, and regenerative potential of pocket implantation of a fish scale in rat model. We report here the safety, detailed morphologic, and physiologic results from the 6-month followup of rabbit model.…”

Section: Introductionmentioning

confidence: 99%

A Cornea Substitute Derived from Fish Scale: 6-Month Followup on Rabbit Model

Yuan

Wang

Lin

et al. 2014

Journal of Ophthalmology

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A fish scale-derived cornea substitute (Biocornea) is proposed as an alternative for human donor corneal tissue. We adopt a regenerative medicine approach to design a primary alternative to the use of fish scale for restoring sight by corneal replacement. Biocornea with corneal multilayer arrangement collagen was implanted to rabbits by pocket implantation. Our study demonstrated the safety and detailed morphologic and physiologic results from the 6 months of followup of rabbit model. In the peripheral Biocornea, the collagen fibrils were arranged in reticular fashion. Slit lamp examination showed that haze and an ulcer were not observed in all groups at 3 months postoperatively while all corneas with Biocornea were clear at both 3 months and 6 months postoperatively. The interface of Biocornea and stromal tissue were filled successfully and without observable immune cells at postoperative day 180. Moreover, the Biocornea was not dissolved and degenerated but remained transparent and showed no apparent fragmentation. Our study demonstrated that the Biocornea derived from fish scale as a good substitute had high biocompatibility and support function after a long-term evaluation. This revealed that the new approach of using Biocornea may yield an ideal artificial cornea substitute for long-term inlay placement.

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A biocornea of fish scales – First results of a research model

Cited by 3 publications

References 0 publications

Fish Scale-Derived Scaffolds for Culturing Human Corneal Endothelial Cells

Fish Scale-Derived Scaffolds for Culturing Human Corneal Endothelial Cells

Biomaterials for corneal endothelial cell culture and tissue engineering

A Cornea Substitute Derived from Fish Scale: 6-Month Followup on Rabbit Model

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