We used stream chemistry and hydrogeomorphology data from 549 stream and 447 river sites to estimate NO 3 -N removal in the Upper Mississippi, Missouri, and Ohio Rivers. We used two N removal models to predict NO 3 -N input and removal. NO 3 -N input ranged from 0.01 to 338 kg km -1 d -1 in the Upper Mississippi River to\0.01-54 kg km -1 d -1 in the Missouri River. Cumulative river network NO 3 -N input was 98700-101676 Mg year -1 in the Ohio River, 85961-89288 Mg year -1 in the Upper Mississippi River, and 59463-61541 Mg year -1 in the Missouri River. NO 3 -N output was highest in the Upper Mississippi River (0.01-329 kg km -1 d -1 ), followed by the Ohio and Missouri Rivers (\0.01-236 kg km -1 d -1 ) sub-basins. Cumulative river network NO 3 -N output was 97499 Mg year -1 for the Ohio River, 84361 Mg year -1 for the Upper Mississippi River, and 59200 Mg year -1 for the Missouri River. Proportional NO 3 -N removal (PNR) based on the two models ranged from \0.01 to 0.28. NO 3 -N removal was inversely correlated with stream order, and ranged from \0.01 to 8.57 kg km -1 d -1 in the Upper Mississippi River to\0.001-1.43 kg km -1 d -1 in the Missouri River. Cumulative river network NO 3 -N removal predicted by the two models was: Upper Mississippi River 4152 and 4152 Mg year -1 , Ohio River 3743 and 378 Mg year -1 , and Missouri River 2277 and 197 Mg year -1 . PNR removal was negatively correlated with both stream order (r = -0.80-0.87) and the percent of the catchment in agriculture (r = -0.38-0.76).