A Bioassay for Plant-Derived Pest Control Agents Using the European Corn Borer12

Freedman, B.; Nowak, L.; Kwolek, W. F.; Berry, E. C.; Guthrie, W. D.

doi:10.1093/jee/72.4.541

Cited by 54 publications

(35 citation statements)

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“…European corn borer egg masses provided newly hatched larvae for this study. The egg masses were obtained over a 24-h period from laboratory colonies reared on an artiÞcial diet (Reed et al 1972, Freedman 1979) from the Corn Insects Research Unit, USDAÐARS, Iowa State University, Ames, and placed in an open transparent plastic bag. Egg masses were maintained under incubation conditions of 25 Ϯ 1ЊC, 40 Ð 60% RH, and a photoperiod of 14:10 (L:D) h, and were observed daily until the proper hatching time when the black larval head capsule became clearly visible.…”

Section: Methodsmentioning

confidence: 99%

Influence of Dietary Applied Monoterpenoids and Derivatives on Survival and Growth of the European Corn Borer (Lepidoptera: Pyralidae)

Lee

Tsao

Coats

1999

Journal of Economic Entomology

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Sixteen natural monoterpenoids and 6 synthetic derivatives were selected for study of larvicidal activity and growth inhibitory effect against the European corn borer, Ostrinia nubilalis (Hübner). For this study, 2 different dietary exposure bioassays were used: compounds applied on the diet surface (on-diet), and compounds incorporated into the diet (in-diet). Most of the monoterpenoid compounds showed some degree of larvicidal activity in both bioassay procedures after a 6-d exposure period. Among the monoterpenoids, pulegone was the most active. Larvicidal toxicities were significantly enhanced for the structurally modified compounds; monoterpenoid derivatives MTEE-25 (2-fluoroethyl thymyl ether) and MTEE-P (propargyl citronellate) were most toxic to borer larvae. When reared on diet containing the monoterpenoids or their derivatives, changes in developmental parameters and pupal weights of the European corn borer also were noted when they were fed several of the compounds. Some larvae reared on treated diet with higher concentrations of the test compounds died before pupating. In general, growth and development of the European corn borer were affected by monoterpenoid compounds, and some compounds such as lmenthol, pulegone, MTEE-25, and MTEE-P acted as insect growth inhibitors. ABSTRACT Sixteen natural monoterpenoids and 6 synthetic derivatives were selected for study of larvicidal activity and growth inhibitory effect against the European corn borer, Ostrinia nubilalis (Hü bner). For this study, 2 different dietary exposure bioassays were used: compounds applied on the diet surface (on-diet), and compounds incorporated into the diet (in-diet). Most of the monoterpenoid compounds showed some degree of larvicidal activity in both bioassay procedures after a 6-d exposure period. Among the monoterpenoids, pulegone was the most active. Larvicidal toxicities were signiÞcantly enhanced for the structurally modiÞed compounds; monoterpenoid derivatives MTEE-25 (2-ßuoroethyl thymyl ether) and MTEE-P (propargyl citronellate) were most toxic to borer larvae. When reared on diet containing the monoterpenoids or their derivatives, changes in developmental parameters and pupal weights of the European corn borer also were noted when they were fed several of the compounds. Some larvae reared on treated diet with higher concentrations of the test compounds died before pupating. In general, growth and development of the European corn borer were affected by monoterpenoid compounds, and some compounds such as l-menthol, pulegone, MTEE-25, and MTEE-P acted as insect growth inhibitors.

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Section: Methodsmentioning

confidence: 99%

Influence of Dietary Applied Monoterpenoids and Derivatives on Survival and Growth of the European Corn Borer (Lepidoptera: Pyralidae)

Lee

Tsao

Coats

1999

Journal of Economic Entomology

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“…Herbs used in this study were pressed according to herbarium techniques and air-dried in the dark at room temperature (RT) and then ground to powders (Melaku., 2008). Solvent extractions were carried out according to the method adopted by Freedman et al (1979) with slight modifications separately within the procedure detailed below. Exactly 50 g of dried powdered plant material was macerated separately in 200 ml of concentrated acetone/ hexane in large labelled glass bottles and put in an orbital shaker for 24h at room temperature.…”

Section: Plant Collection and Preparation Of Extractsmentioning

confidence: 99%

Peliminary examination of herbal extracts on the inhibition of <i>Helicobacter Pylori</i>

Guzeldag¹,

Kadioglu²,

Mercimek³

et al. 2013

Afr. J. Trad. Compl. Alt. Med.

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Background: This work aims at describing the traditional uses, to determine the antimicrobial potential of two different extracts hexane, acetone of the leaves of Citrus unshiu, Citrus sinensis, Citrus limon, Laurus nobilis, Citrus paradisi on clinical strain of H. pylori in a bid to identify potential sources of cheap starting materials for the synthesis of new drugs. H. pylori strain was a culture collection of Hacettepe University, Turkey. Methods: The activity was quantitatively assessed on the basis of the inhibition zone, and their activity index was also calculated along with the MIC method.

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“…The method of Freedman et al, [18] was adopted with minor modification. Samples of 100 g plant materials were extracted in a Soxhlet apparatus, using ethanol (75%) as solvent at a rate of 3 ml/g plant material and for 8 h extraction period.…”

Section: Extractionmentioning

confidence: 99%

Larvicidal Activity of Some Botanical Extracts, Commercial Insecticides and their Binary Mixtures Against the Housefly, Musca Domestica L.

Mansour¹

2011

TOTNJ

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Following preliminary screening of 13 ethanolic plant extracts, belonging to 10 different families, a total of 11 extracts were subjected to detailed toxicity evaluation against the larval stage of the housefly, Musca domestica L. The larvicidal LC 50 values were < 100 ppm for Piper nigrum (50.1 ppm), Azadirachta indica (76.9 ppm), Conyza aegyptiaca (77.0 ppm) and Cichorium intybus (96.8 ppm); representing the highest potent extracts among the bioassayed candidates. Punica granatum extract exhibited the lowest toxicity (213.9 ppm). In comparison, commercial insecticides showed superior larvicidal toxicity; accounting to the following LC 50 values: 0.029, 0.03, 0.61 and 0.64 ppm for deltamethrin, methomyl, chlorpyrifos and flufenoxuron, respectively. Combining botanical extracts with insecticides, at equitoxic dosages (e.g., LC 25 values), induced potentiating effects for a 44 bioassayed mixtures against the housefly larvae. Moreover, mixing the insecticides at LC 0 (a concentration level causing no observed mortality) with the LC 50 of each of the plant extracts have resulted in 44 paired combinations. Mostly, the "synergistic factor; S.F." ranged between 1.6 -1.9; giving rise to high synergistic effects. Specifically, the synergistic effect was much pronounced for mixtures of the insecticide deltamethrin with different botanical extracts. Most of the tested toxicants induced different forms of developmental effects after exposure of 3 rd larval instars to sublethal concentrations (LC 25 ppm). Larvae treated with A. indica, Citrus aurantifolia, Eucalyptus globulus (leaves or fruits), P. granatum, Salix safsaf, Sonchus oleraceus, Zea mays, as well as the insecticides chlorpyrifos, deltamethrin and methomyl failed to develop into adult stages. Morphologically, there were different forms of pupal and adult abnormalities, where the treatments of P. granatum and S. oleraceus caused abnormal pupal size in addition to pupal-adult intermediate. In the resulted Musca domestica adults, the effects were seen as one-winged insects, small size, compressed body and abdomen elongation. Such deformations were attributed to treatments of C. intybus, C. aegyptiaca, Piper nigrum and the IGR flufenoxuron. The overall results of the present investigation reveal the broad-spectrum toxic properties of the tested plant extracts against Musca domestica larvae; which may encourage further research on housefly control in tropics using indigenous plants.

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A Bioassay for Plant-Derived Pest Control Agents Using the European Corn Borer12

Cited by 54 publications

References 0 publications

Influence of Dietary Applied Monoterpenoids and Derivatives on Survival and Growth of the European Corn Borer (Lepidoptera: Pyralidae)

Influence of Dietary Applied Monoterpenoids and Derivatives on Survival and Growth of the European Corn Borer (Lepidoptera: Pyralidae)

Peliminary examination of herbal extracts on the inhibition of <i>Helicobacter Pylori</i>

Larvicidal Activity of Some Botanical Extracts, Commercial Insecticides and their Binary Mixtures Against the Housefly, Musca Domestica L.

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