A bioartificial dermal regeneration template promotes skin cell proliferation in vitro and enhances large skin wound healing in vivo

Abstract

A novel bioartificial dermal regeneration template has been developed using platelet-rich plasma and acellular animal skin collagen sponge for the treatment of larger area and full thickness skin wounds. This platelet-rich plasma-collagen sponge keeps native skin structure and contains huge amounts of growth factors. The effect of this bioartificial dermal regeneration template was tested in vitro and in vivo via a mimic poor wound healing process by adding collagenase I into cell culture medium or the wound area. The in vitro experimental results indicated that the rat skin cells grew faster and produced more collagen in platelet-rich plasma-collagen sponge with collagenase than those treated either with collagen sponge plus collagenase, or collagenase, or control group without treatment. The in vivo experiments were performed by large rat skin wounds, 1.5 cm diameter, treated either with collagenase, or collagenase plus collagen sponge, or collagenase plus platelet-rich plasma-collagen sponge. The wound without treatment was used as a control. The wounds treated with collagenase-containing platelet-rich plasma-collagen sponge healed 4 times faster than the untreated wounds, 6 times faster than the collagenase treated wounds, 2.4 times faster than collagenase-containing collagen sponge treated wounds. The immunostaining indicated that the healed tissues in the wound areas treated with collagenase-containing platelet-rich plasma-collagen sponge were composed of collagen type I and collagen III with blood vessels and hair follicles. The results demonstrated that this collagenase-containing platelet-rich plasma-collagen sponge works as a bioartificial dermal regeneration template. The application of this collagenase-containing platelet-rich plasma-collagen sponge promotes the traumatic skin wound healing and permits the reconstitution of the inherent barrier functions of the skin.

Efficacy of application of Equisetum pyramidale Goldm. hydrogel for tissue restoration of induced skin lesions in Wistar rats

“…The healing process has the objective of recovering the integrity of the damaged tissue and subsequently overlapping phases, among them: inflammatory, proliferative and remodeling (Hosgood, 2006;Chang et al, 2017;Yeh et al, 2017).…”

The Devices, Experimental Scaffolds, and Biomaterials Ontology (DEB): A Tool for Mapping, Annotation, and Analysis of Biomaterials Data

“…For example, a scaffold may be named with few or a many technical details in the name, with emphasis on materials used (biomaterials or biologically active substances) engineered features (such as structure, architecture, or a processing technique), its medical application, or a biological aspect of its performance, depending on the author's preference. Examples of manufactured objects' naming in the gold standard set include: “Electrospun polycaprolactone nanofibers decorated by drug loaded chitosan nano‐reservoirs for antibacterial treatments,” [ 22 ] which gives information about manufacture technique, materials, structures, and medical application, but also: “a silver‐releasing foam dressing in venous leg ulcer healing,” [ 23 ] naming the medical application, structure, and the bioactive substance, or alternatively: “A bioartificial dermal regeneration template,” [ 24 ] which only names the manufactured object and its medical application. All these articles go on to describe the manufactured object in more detail, often naming it using different variations.…”

“…For instance, in the latest example, the dermal template is also called “platelet‐rich plasma‐collagen sponge” and “collagenase‐containing platelet‐rich plasma‐collagen sponge” in the abstract text. [ 24 ] Using the DEB ontology, this associated information can be cataloged into a class and linked to the manufactured object via the designated object properties. Thus, a single manufactured object will be defined by multiple, separate features, and data from testing it can be compared based on those features.…”

“…The ontology was developed using Protégé(19), which is a free and open source ontology development tool. [ 24,43 ] The methodology developed to curate terms into the ontology is described in detail in results' section. To avoid the curation of terms already well‐organized in other lexical sources, the existence of key terms in other terminologies and ontologies was searched using the Unified Medical Language System (UMLS) Metathesaurus Browser (uts.nlm.nih.gov/metathesaurus.html), the bioportal ontology recommender, [ 44 ] the OBO Foundry (https://obofoundry.org/), and the ontobee server (https://www.ontobee.org/).…”

A new nanoscale transdermal drug delivery system: oil body-linked oleosin-hEGF improves skin regeneration to accelerate wound healing

“…hEGF is able to bind to EGFR on the cell membrane near the wound [ 5 ], activates the protein kinase, and thus accelerates the synthesis of DNA, RNA and protein. Ultimately they promote the cells near wound to speed up the mitosis [ 6 ], making the wound to heal effectively [ 7 ].…”