A bio-inspired microfluidic concentrator for regenerated silk fibroin solution

Luo, Jie; Zhang, Yaopeng; Huang, Yuancan; Shao, Huili; Hu, Xuechao

doi:10.1016/j.snb.2011.12.093

Cited by 26 publications

(18 citation statements)

References 25 publications

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“…Silk affords excellent physical and chemical properties, such as stability in salts and various pHs, while also allowing for chemical modification . Attempts to recapitulate native mechanical properties have been investigated with regenerated silk fibroin via electrospinning, wetspinning, dry spinning, and microfluidic silk spinning . Although these silk fabrication techniques can achieve crystallinity content in line with that of native silk, thus affording great strength, there remains a limitation with fabricating a single fiber that provides high strength and extensibility.…”

Section: Introductionmentioning

confidence: 99%

“…Upon fabrication, these fibers can be used for bottom‐up assembly for 3D scaffolds via weaving, braiding, or knitting . Many polymers have been used in the above fabrication methods, including silk, collagen, cellulose, polylactic acid, poly(lactic‐ co ‐glycolic acid), polycaprolactone, acrylamine poly(ethylene glycol), polyglycolide, and poly(L/DL lactide) …”

Section: Introductionmentioning

confidence: 99%

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Fabrication of elastomeric silk fibers

et al. 2017

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Methods to generate fibers from hydrogels, with control over mechanical properties, fiber diameter and crystallinity, while retaining cytocompatibility and degradability, would expand options for biomaterials. Here we exploited features of silk fibroin protein for the formation of tunable silk hydrogel fibers. The biological, chemical, and morphological features inherent to silk were combined with elastomeric properties gained through enzymatic crosslinking of the protein. Post-processing via methanol and autoclaving provided tunable control of fiber features. Mechanical, optical, and chemical analyses demonstrated control of fiber properties by exploiting the physical cross-links, and generating double network hydrogels consisting of chemical and physical cross-links. Structure and chemical analysis revealed crystallinity from 30–50%, modulus from 0.5MPa to 4MPa, and ultimate strength 1 to 5 MPa depending on the processing method. Fabrication and post-processing combined provided fibers with extensibility from 100 to 400% ultimate strain. Fibers strained to 100% exhibited 4th order birefringence, revealing macroscopic orientation driven by chain mobility. The physical cross-links were influenced in part by the drying rate of fabricated materials, where bound water, packing density, and micro-structural homogeneity influenced cross-linking efficiency. The ability to generate robust and versatile hydrogel microfibers is desirable for bottom-up assembly of biological tissues and for broader biomaterial applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fabrication of elastomeric silk fibers

et al. 2017

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“…The silk fibroin solution was produced according to the method reported previously. Bombyx mori silkworm was boiled in Na 2 CO 3 aqueous solution (0.5 wt %) at 100 °C for 30 min and washed with deionized water thoroughly to remove the sericin . The degummed silk was then dissolved in 9.5 mol/L LiBr aqueous solution at 40 °C for 2 h. The solution was dialyzed against deionized water with a semipermeable membrane (14 kDa cutoff) for 3 days and concentrated.…”

Section: Methodsmentioning

confidence: 99%

Fabrication of silk fibroin film with properties of thermal insulation and temperature monitoring

Zhao

Wang

Luo

et al. 2016

J. Polym. Sci. Part B: Polym. Phys.

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Silk fibroin exhibits excellent mechanical properties, good biocompatibility, and biodegradability combined with benign processing conditions, attracting considerable research interest for the application as biomedical materials. Among the diverse forms of sponges, hydrogels, films, and mats manufactured from silk fibroin, films are especially appealing due to the high water and oxygen permeability, good cell attachment, and low immunogenicity. Fabrication of silk fibroin films with novel properties has been successfully developed simply by incorporating various functional components into it. In the present study, the properties of thermal insulation and temperature monitoring for the silk fibroin film are demonstrated for the first time through the incorporation of thermochromic microcapsules within it. Moreover, the silk fibroin film is also endowed with improved mechanical properties in terms of tension strength and elongation at break because of the reinforcing effect of thermochromic microcapsules. The silk fibroin film fabricated with novel features in this study can be a good candidate for the application of wound dressings, tissue engineering scaffolds, and bio-related devices in the future.

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“…According to the method reported previously, [7] cocoons of Bombyx mori were used to prepare the RSF aqueous solution for dry-spinning, microfluidic spinning and electrospinning. The asspun fibers or mats were post-drawn in 80-90 vol% ethanol aqueous solution, and then immersed in the solution for certain minutes or hours.…”

Section: Methodsmentioning

confidence: 99%

“…Based on photolithography process and plasma bonding technology, [7,8] microfluidic chips of poly(dimethylsiloxane) (PDMS) (Dow Corning Inc., Midland MI) were fabricated. For multilayered microchip, a cellulose dialysis membrane was bonded between two PDMS layers with epoxy resin.…”

Section: Methodsmentioning

confidence: 99%

Artificial Silk Materials with Enhanced Mechanical Properties and Controllable Structures

Zhang

Pan

Luo

et al. 2014

Int.J.Soc.Mater.Eng.Resour.

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Spider and silkworm produce animal silks exhibiting outstanding mechanical properties by using smart spinning method. In this paper, a biomimetic spinning of spider silk and silkworm silk was studied from various routes. Regenerated silk fibroin (RSF) aqueous solution was firstly dry-spun to artificial fiber in air at room temperature as the animal silks were made naturally. The conformation transition of the silk fibroin was then induced by post-drawing in ethanol aqueous solution. The oriented crystalline and amorphous regions of the silk fibers contribute to the remarkable mechanical properties of the artificial silk, which exceed those of natural silkworm silk. By mimicking the functions of the spinning apparatus of spider and silkworm, ion and protein concentrations in the RSF aqueous solution were adjusted in microfluidic chips with multiple channels. Inspired by the shape and dimensions of the natural spinning apparatus, a microfluidic chip was designed and applied to the studies of aggregation mechanism of silk fibroin in micro-channel. Moreover, the supermolecular structures of silk fibroin were effectively controlled to reinforce dry-spun/electrospun fibers of RSF by mimicking the core-shell structure of natural animal silks, adding silk sericin or carbon nanotube in spinning solutions and changing the collecting method in electrospinning process.

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A bio-inspired microfluidic concentrator for regenerated silk fibroin solution

Cited by 26 publications

References 25 publications

Fabrication of elastomeric silk fibers

Fabrication of elastomeric silk fibers

Fabrication of silk fibroin film with properties of thermal insulation and temperature monitoring

Artificial Silk Materials with Enhanced Mechanical Properties and Controllable Structures

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