A BIO-ASSAY FOR MINUTE AMOUNTS OF PROGESTERONE<sup>1</sup>

Hooker, Charles W.; Forbes, Thomas R.

doi:10.1210/endo-41-2-158

Cited by 144 publications

(21 citation statements)

References 9 publications

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“…Until quite recently, the only method available for measuring progesterone in the peripheral blood was the bio-assay devised by Hooker & Forbes (1947). Using this technique, blood progesterone levels have been determined throughout pregnancy in women, rhesus monkeys (Forbes, 1951), sheep (Neher & Zarrow, 1954), rabbits (Zarrow & Neher, 1955) and mice (Forbes & Hooker, 1957) ; of all these species, the only one to show a fall in the circulating level prior to parturition was the mouse.…”

mentioning

confidence: 99%

Blood Progesterone Levels in Relation to Parturition

Short¹

1960

Reproduction

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mentioning

confidence: 99%

Blood Progesterone Levels in Relation to Parturition

Short¹

1960

Reproduction

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“…Samples were subjected to the Hooker-Forbes bioassay technique [Hooker & Forbes, 1947] which involves the introduction of plasma into a tied-off section of the uterus of an ovariectomized mouse. If progestins are present there is a characteristic response by the stromal nuclei.…”

Section: Methodsmentioning

confidence: 99%

The Post-Nuptial Occurrence of Progestins in the Seminiferous Tubules of Birds

Lofts¹,

Marshall²

1959

Journal of Endocrinology

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It has been previously suggested that the cholesterol which appears during the post-nuptial metamorphosis of the seminiferous tubules of submammalian vertebrates may be of endocrine significance. It is now shown by means of paper chromatography that the tubular lipids of pigeons probably contain progesterone and its metabolites. The testes of control ('pre-nuptial') birds contain androgens but no progestins.Again, the Hooker-Forbes bioassay test indicates the presence of plasma progestins in seven out of eight birds with metamorphosed lipoidal tubules. Of seven control birds in full spermatogenesis six were negative for progestin. The possibility that the regular, post-nuptial accumulation and expenditure of tubular cholesterol is functionally homologous with the formation and regression of the corpus luteum is discussed.At the end of the seasonal spermatogenesis the seminiferous tubules of wild birds undergo a metamorphosis involving a massive steatogenesis and the profuse appear¬ ance of cholesterol. During the succeeding months the tubular lipids and their cholesterol disappear, the rate varying from species to species [Marshall, 1949[Marshall, , 1950. Since cholesterol is associated with the production of steroid hormones in the neighbouring interstitium, and probably in the adrenal cortex, it may be equally so when quantities of it appear suddenly and cyclically in the seminiferous tubules, and then disappear. In short, the metamorphosed tubules may have a temporary endo¬ crine function.

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“…Similarly, the diameters of the corpora lutea provided information on the growth and regression of the corpora lutea of lactation and pregnancy. Hypertrophy of the stromal nuclei of the endometrium indicated progestin, and the height of the uterine epithelium or stromal oedema the presence of oestrogen [Hooker, 1945;Hooker & Forbes, 1947]. Cornification or epithelial prohferation of the vagina revealed the presence of oestrogen, and mucification the interaction of oestrogen and progesterone [Klein, 1937;Seegar-Jones & Astwood, 1942].…”

Section: Methodsmentioning

confidence: 99%

“…The follicles are therefore small and the secretion of oestrogen is negligible during the first half of lactation. Between 10 and 15 days post partum, for unknown reasons, the level of prolactin drops [Hurst & Turner, 1942]. Although the corpus luteum is still func¬ tional, progesterone declines sufficiently to permit the release of additional gonado¬ trophins.…”

Section: Histology Of the Uterus During Lactationmentioning

confidence: 99%

A Histological Study of the Reproductive Tract of the Lactating Mouse

Greenwald¹

1958

Journal of Endocrinology

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The histology of the reproductive tract of the lactating mouse is described.During the first 11 days post partum, the largest vesicular follicles are 350\g=m\ in diameter, the corpora lutea of pregnancy are larger than the corpora lutea of lactation, and the vagina has a two-layered epithelium. Significant changes occur after 12 days post partum: the size of the follicles in most animals increases to 400-450\g=m\, the corpora lutea of lactation are larger than the old set, and the vagina becomes mucified. This suggests that lactation in the mouse is divided into two periods: the first in which progesterone and negligible amounts of oestrogen are present lasts until the 11th day. This is followed by a period of increasing levels of oestrogen and a gradual subsidence in that of progesterone.A hypothesis is proposed to account for the morphological changes that occur during lactation.

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A BIO-ASSAY FOR MINUTE AMOUNTS OF PROGESTERONE¹

Cited by 144 publications

References 9 publications

Blood Progesterone Levels in Relation to Parturition

Blood Progesterone Levels in Relation to Parturition

The Post-Nuptial Occurrence of Progestins in the Seminiferous Tubules of Birds

A Histological Study of the Reproductive Tract of the Lactating Mouse

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A BIO-ASSAY FOR MINUTE AMOUNTS OF PROGESTERONE1

Cited by 144 publications

References 9 publications

Blood Progesterone Levels in Relation to Parturition

Blood Progesterone Levels in Relation to Parturition

The Post-Nuptial Occurrence of Progestins in the Seminiferous Tubules of Birds

A Histological Study of the Reproductive Tract of the Lactating Mouse

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A BIO-ASSAY FOR MINUTE AMOUNTS OF PROGESTERONE¹