A bienzymatic biocatalyst constituted by glucose oxidase and Horseradish peroxidase immobilized on ordered mesoporous silica

Lipase AK from Pseudomonas fluorescens and Lipase RM from Rhizomucor miehei were encapsulated into a zeolite imidazolate framework (ZIF‐8) by a “one‐pot” synthesis to obtain AK@ZIF‐8 and RM@ZIF‐8 biocatalysts. The effect of a high (1:40) and low (1:4) Zn/2‐methylimidazole molar ratio on the biocatalysts synthesis was investigated. The different Zn/ligand (L) ratios affected both the surface area, the loading, and the specific activity of the biocatalysts. Samples synthesized by using a high Zn/L ratio had high values of surface area whereas those obtained by using a low Zn/L ratio had higher loadings and specific activities. The decrease of pH (from 11.6 to 9.4) during the synthesis at high Zn/L ratio produced ZIF‐8 samples with features similar to those observed for low Zn/L ratio samples. The low Zn/L (1:4) ratio AK@ZIF‐8 biocatalyst retained 99 % activity after storage for 15 days at 5 °C and 40 % activity after five reaction cycles.

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“…Protein loading (mg protein g ZIF‐8 −1 ) is the amount of immobilized protein per g of support [Eq. ]:

true normall normalo normala normald normali normaln normalg = \frac{{[P]}_{0} V_{0} - {[P]}_{f} V_{f} - {[P]}_{w} V_{w}}{m_{Z I F - 8}}

…”

Section: Methodsmentioning

confidence: 99%

Lipase Encapsulation onto ZIF‐8: A Comparison between Biocatalysts Obtained at Low and High Zinc/2‐Methylimidazole Molar Ratio in Aqueous Medium

et al. 2018

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“…2013, Issue 9). 3D‐mesopore systems are suitable for catalysis because they are less plagued by pore‐blocking, while 1D systems are often preferred for encapsulating biomolecules, drugs, and confined nanostructures, such 1D‐metal nanowires ,,…”

Section: Empty Space Architecturesmentioning

confidence: 99%

“…2013, Issue 9). 3D-mesopore systems are suitable for catalysis because they are less plagued by pore-blocking, while 1D systems are often preferred for encapsulating biomolecules, [354][355][356] drugs, [357] and confined nanostructures, such 1D-metal nanowires. [349,358,359] MCM-41 and SBA-15 have non-intersecting one-dimensional channels with long-range hexagonal order and well-defined diameters ( Figure 13).…”

Section: Mesoporous Silica Materialsmentioning

confidence: 99%

Supramolecular Organization in Confined Nanospaces

Tabacchi

2018

ChemPhysChem

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Empty spaces are abhorred by nature, which immediately rushes in to fill the void. Humans have learnt pretty well how to make ordered empty nanocontainers, and to get useful products out of them. When such an order is imparted to molecules, new properties may appear, often yielding advanced applications. This review illustrates how the organized void space inherently present in various materials: zeolites, clathrates, mesoporous silica/organosilica, and metal organic frameworks (MOF), for example, can be exploited to create confined, organized, and self-assembled supramolecular structures of low dimensionality. Features of the confining matrices relevant to organization are presented with special focus on molecular-level aspects. Selected examples of confined supramolecular assemblies - from small molecules to quantum dots or luminescent species - are aimed to show the complexity and potential of this approach. Natural confinement (minerals) and hyperconfinement (high pressure) provide further opportunities to understand and master the atomistic-level interactions governing supramolecular organization under nanospace restrictions.

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“…Another very recent example on enzyme immobilization within MSNs through covalent bonding was reported by Pitzalis et al The authors reported an active immobilized cascade reaction by employing the enzymatic tandem pair consisting of GOx and HRP. The two enzymes were covalently attached into the MSNs making use of APTES and GA to immobilize a mixture of the two enzymes .…”

Section: Micro/nanosized Single‐compartment Enzymatic Reactorsmentioning

confidence: 99%

Recent Progress in Micro/Nanoreactors toward the Creation of Artificial Organelles

Godoy-Gallardo

York-Durán

Hosta‐Rigau

2017

Adv Healthcare Materials

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Artificial organelles created from a bottom up approach are a new type of engineered materials, which are not designed to be living but, instead, to mimic some specific functions inside cells. By doing so, artificial organelles are expected to become a powerful tool in biomedicine. They can act as nanoreactors to convert a prodrug into a drug inside the cells or as carriers encapsulating therapeutic enzymes to replace malfunctioning organelles in pathological conditions. For the design of artificial organelles, several requirements need to be fulfilled: a compartmentalized structure that can encapsulate the synthetic machinery to perform an enzymatic function, as well as a means to allow for communication between the interior of the artificial organelle and the external environment, so that substrates and products can diffuse in and out the carrier allowing for continuous enzymatic reactions. The most recent and exciting advances in architectures that fulfill the aforementioned requirements are featured in this review. Artificial organelles are classified depending on their constituting materials, being lipid and polymer-based systems the most prominent ones. Finally, special emphasis will be put on the intracellular response of these newly emerging systems.

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A bienzymatic biocatalyst constituted by glucose oxidase and Horseradish peroxidase immobilized on ordered mesoporous silica

Cited by 61 publications

References 49 publications

Lipase Encapsulation onto ZIF‐8: A Comparison between Biocatalysts Obtained at Low and High Zinc/2‐Methylimidazole Molar Ratio in Aqueous Medium

Lipase Encapsulation onto ZIF‐8: A Comparison between Biocatalysts Obtained at Low and High Zinc/2‐Methylimidazole Molar Ratio in Aqueous Medium

Supramolecular Organization in Confined Nanospaces

Recent Progress in Micro/Nanoreactors toward the Creation of Artificial Organelles

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