A bibliography of the marine mud snail<i>Ilyanassa obsoleta</i>

Collier, Justine

doi:10.1080/07924259.2002.9652766

Cited by 7 publications

(7 citation statements)

References 205 publications

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“…Ilyanassa obsoleta adults obtained from the Marine Resources Center (Marine Biological Laboratories, Woods Hole, MA, USA) were kept in 76 l tanks (ASW, Instant Ocean) with 3 cm of aquarium rock substrate, sponge filtration and bubbler aeration at room temperature (RT, 23±1 ° C) and fed frozen clam meat every other day. Embryos were collected from newly deposited egg capsules as described previously ( Collier, 1981 ), and cultured at 23±1 ° C in groups of three to 15 in 35 mm plastic Petri dishes with 2-3 ml of 0.2 μm-filtered artificial sea water (FASW, Instant Ocean). For longer incubation, penicillin (100 U/ml) and streptomycin (200 μg/ml) were added, and embryos were moved to fresh FASW in a new dish every 2 days ( Gharbiah et al, 2009 ).…”

Section: Methodsmentioning

confidence: 99%

“…To isolate the A or B quadrants, we lysed the CD cell (mother of the C and D quadrant founder cells) in the same manner at the two-cell stage, and then lysed one of the two indistinguishable remaining cells (A or B) shortly after their formation at second cleavage. For PL(−) quadrant isolations, PLs were removed at the trefoil stage (first cleavage) by gentle agitation in low-Ca 2+ /Mg 2+ FASW ( Collier, 1981 ; Lambert and Nagy, 2001 ). Under these conditions, the PL spontaneously separates and falls away upon full constriction of the furrow.…”

Section: Methodsmentioning

confidence: 99%

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Embryonic organizer specification in the mud snail Ilyanassa obsoleta depends on intercellular signaling

Wandelt,

Nakamoto,

Goulding

et al. 2023

Development

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In early embryos of the caenogastropod snail Ilyanassa obsoleta, cytoplasmic segregation of a polar lobe is required for establishment of the D quadrant founder cell, empowering its great-granddaughter macromere 3D to act as a single-celled organizer that induces ectodermal pattern along the embryo's secondary body axis. We present evidence that polar lobe inheritance is not sufficient to specify 3D potential, but rather makes the D macromere lineage responsive to some intercellular signal(s) required for activating the special properties of 3D. Experimental removal of micromeres results in loss of organizer-linked MAPK activation, complete and specific defects of organizer-dependent larval organs, and progressive cell cycle retardation leading to equalization of the normally accelerated division schedule of 3D. Ablation of the second-quartet micromere 2d greatly potentiates the effects of first-quartet micromere ablation. Our findings link organizer activation in I. obsoleta to the putative ancestral spiralian mechanism in which a signal from micromeres leads to specification of 3D among four initially equivalent macromeres.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Embryonic organizer specification in the mud snail Ilyanassa obsoleta depends on intercellular signaling

Wandelt,

Nakamoto,

Goulding

et al. 2023

Development

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“…The embryos undergo the classic pattern of spiral cleavage (Fig. 1C-J), and a large body of descriptive and experimental literature is available to guide molecular analyses (see Collier 2002). For instance, the larval fate maps of the micromeres of the 25-cell embryo have been determined (Render 1991(Render , 1997, and the phenotypic effects of deleting each micromere at this stage have been reported (Clement 1967(Clement , 1986aSweet 1998).…”

Section: Uses Of Ilyanassa As a Model Systemmentioning

confidence: 98%

The Snail Ilyanassa: A Reemerging Model for Studies in Development: Figure 1.

Gharbiah¹,

Cooley²,

Leise³

et al. 2009

Cold Spring Harb Protoc

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Ilyanassa obsoleta is a marine gastropod that is a long-standing and very useful model for studies of embryonic development. It is especially important as a model for the spiralian development program, a distinctive mode of early development shared by a large group of animal phyla, but poorly understood. Ilyanassa adults are readily obtainable and easy to keep in the laboratory, and they produce large numbers of embryos throughout most of the year. The embryos are amenable to classic embryological manipulation techniques as well as a growing number of molecular approaches. In this article, we present an overview of aspects of its biology and use as a model organism.

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“…Embryos of both species were collected and handled in the same fashion (Collier, 1981). None of the embryos in this study differed significantly in early cell cycle timing or the onset timing of cleavage with respect to oviposition.…”

Section: Embryo Culture and Imagingmentioning

confidence: 99%

Acclimation of intertidally reproducing sea‐snails protects embryos from lethal effects of transient hyperthermia

Goulding

2024

J Exp Zool Pt B

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Embryos of Ilyanassa obsoleta (from Massachusetts and Florida) and Phrontis vibex (from Florida) were exposed to temperatures from 33 to 37°C. In both species, very young embryos are especially sensitive to thermal stress. Brief early heat shock did not disturb spiral cleavage geometry but led to variable, typically severe defects in larval morphogenesis and tissue differentiation. In Ilyanassa but not P. vibex, early heat shock resulted in immediate slowing or arrest of interphase progression during early cleavage. This reversible arrest was correlated with improved prognosis for larval development and (in Massachusetts snails, at least) depended on parental acclimation to warm temperature (~25.5°C). Embryos from Massachusetts snails housed at lower temperature (16°C) exhibited cytokinesis failure when briefly incubated at 33°C during early cleavage, and tissue differentiation failure during incubation at 33°C begun at later stages. This preliminary study reveals a case in which stress‐conditioned parents may endow embryos with protection against potentially lethal thermal stress during the most vulnerable stages of life.

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A bibliography of the marine mud snailIlyanassa obsoleta

Cited by 7 publications

References 205 publications

Embryonic organizer specification in the mud snail Ilyanassa obsoleta depends on intercellular signaling

Embryonic organizer specification in the mud snail Ilyanassa obsoleta depends on intercellular signaling

The Snail Ilyanassa: A Reemerging Model for Studies in Development: Figure 1.

Acclimation of intertidally reproducing sea‐snails protects embryos from lethal effects of transient hyperthermia

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